Rabbit Polyclonal BAF170 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human SMARCC2 aa 1150 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SMARCC2 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TAQ2
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-5.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:11018012). Can stimulate the ATPase activity of the catalytic subunit of these complexes (PubMed:10078207). May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells (PubMed:12192000). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (By similarity).
Alternative names
BAF170, SMARCC2, SWI/SNF complex subunit SMARCC2, BRG1-associated factor 170, SWI/SNF complex 170 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2
Recommended products
Rabbit Polyclonal BAF170 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human SMARCC2 aa 1150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SMARCC2 aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8TAQ2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BAF170 also known as SMARCC2 is a protein that plays an essential role in chromatin remodeling. The molecular mass of BAF170 is approximately 131 kDa. It is expressed in various tissues including the brain and heart where it helps in altering chromatin structure to allow for transcriptional regulation. The protein contributes to the regulation of gene expression by modulating the accessibility of chromatin-bound transcription factors.
- Biological function summary
BAF170 forms an integral part of the SWI/SNF chromatin remodeling complex which influences how genes are expressed in the cell. This complex alters the position of nucleosomes enabling the access of transcriptional machinery to DNA. Through this action BAF170 regulates the expression of genes necessary for processes such as cell differentiation and proliferation. The SWI/SNF complex interacts with multiple other proteins creating a dynamic regulatory environment for gene expression.
- Pathways
BAF170 participates in significant cellular pathways like the Wnt signaling pathway and the Hedgehog pathway. These pathways influence many vital cellular processes including development and cell cycle regulation. In the Wnt pathway BAF170 works in concert with other proteins such as beta-catenin to regulate target gene expression. Similarly in the Hedgehog pathway BAF170 interacts with proteins involved in the signaling cascade affecting cellular responses and developmental processes.
- Associated diseases and disorders
Aberrations in BAF170 activity associate with certain cancers and neurodevelopmental disorders. Alterations in the SWI/SNF complex's function where BAF170 is a central component can result in malignant transformations leading to tumors. Moreover disruptions in BAF170's regulation are linked to issues impacting brain development possibly influencing conditions such as autism spectrum disorders. Researchers continue to investigate the protein's role in other pathological states providing insight into its contribution to disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-BAF170 antibody (ab264170)
4-8% SDS-PAGE
All lanes: Western blot - Anti-BAF170 antibody (ab264170) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Lane 4: NIH/3T3 whole cell lysate at 50 µg
SecondaryAll lanes: Goat anti-Rabbit Light Chain HRP Conjugate
Predicted band size: 133 kDa
Exposure time: 1s
Immunoprecipitation - Anti-BAF170 antibody (ab264170)
BAF170 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264170 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264170 at 1 μg/ml.
Lane 1: ab264170 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
All lanes: Immunoprecipitation - Anti-BAF170 antibody (ab264170)
Predicted band size: 133 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody (ab264170)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin basal cell carcinoma tissue labeling BAF170 with ab264170 at 1/200 dilution. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com