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AB243634

Anti-BAF170 antibody [EPR23066-21]

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(1 Publication)

Rabbit Recombinant Monoclonal BAF170 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BAF170, SMARCC2, SWI/SNF complex subunit SMARCC2, BRG1-associated factor 170, SWI/SNF complex 170 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2

8 Images
Western blot - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • WB

Lab

Western blot - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody also recognizes weak unidentifiable bands below 100kDa.

All lanes:

Western blot - Anti-BAF170 antibody [EPR23066-21] (ab243634) at 1/1000 dilution

Lane 1:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 2:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 5:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 10 µg

Lane 7:

Mouse lung tissue lysate at 10 µg

Lane 8:

Rat brain tissue lysate at 10 µg

Lane 9:

Rat liver tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 133 kDa

Observed band size: 170 kDa

false

Exposure time: 3s

Western blot - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • WB

Lab

Western blot - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody also recognizes weak unidentifiable bands below 100kDa.

All lanes:

Western blot - Anti-BAF170 antibody [EPR23066-21] (ab243634) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell at 10 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 133 kDa

Observed band size: 170 kDa

false

Exposure time: 3s

Immunoprecipitation - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • IP

Unknown

Immunoprecipitation - Anti-BAF170 antibody [EPR23066-21] (AB243634)

BAF170 was immunoprecipitated from 0.35 mg K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10ug with ab243634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243634 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10ug

Lane 2 : ab243634 IP in K-562 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243634 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

All lanes:

Immunoprecipitation - Anti-BAF170 antibody [EPR23066-21] (ab243634)

Predicted band size: 133 kDa

Observed band size: 170 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labeling BAF170 with ab243634 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in A549 cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

PBS only control : Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labeling BAF170 with ab243634 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in Neuro-2a cell line. The nuclear counter stain is DAPI (Blue). Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).

PBS only control : Used PBS instead of primary antibody, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling BAF170 with ab243634 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus (PMID : 27392482) The section was incubated with ab243634 for 15 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

Flow Cytometry (Intracellular) - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A549 (human lung carcinoma epithelial cell) cells labelling BAF170 with ab243634 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

Flow Cytometry (Intracellular) - Anti-BAF170 antibody [EPR23066-21] (AB243634)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BAF170 antibody [EPR23066-21] (AB243634)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling NCR3 with ab243649 at 1/700 compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243634).

  • Carrier free

    Anti-BAF170 antibody [EPR23066-21] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23066-21

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, WB, Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAF170 also known as SMARCC2 is a protein that plays an essential role in chromatin remodeling. The molecular mass of BAF170 is approximately 131 kDa. It is expressed in various tissues including the brain and heart where it helps in altering chromatin structure to allow for transcriptional regulation. The protein contributes to the regulation of gene expression by modulating the accessibility of chromatin-bound transcription factors.
Biological function summary

BAF170 forms an integral part of the SWI/SNF chromatin remodeling complex which influences how genes are expressed in the cell. This complex alters the position of nucleosomes enabling the access of transcriptional machinery to DNA. Through this action BAF170 regulates the expression of genes necessary for processes such as cell differentiation and proliferation. The SWI/SNF complex interacts with multiple other proteins creating a dynamic regulatory environment for gene expression.

Pathways

BAF170 participates in significant cellular pathways like the Wnt signaling pathway and the Hedgehog pathway. These pathways influence many vital cellular processes including development and cell cycle regulation. In the Wnt pathway BAF170 works in concert with other proteins such as beta-catenin to regulate target gene expression. Similarly in the Hedgehog pathway BAF170 interacts with proteins involved in the signaling cascade affecting cellular responses and developmental processes.

Aberrations in BAF170 activity associate with certain cancers and neurodevelopmental disorders. Alterations in the SWI/SNF complex's function where BAF170 is a central component can result in malignant transformations leading to tumors. Moreover disruptions in BAF170's regulation are linked to issues impacting brain development possibly influencing conditions such as autism spectrum disorders. Researchers continue to investigate the protein's role in other pathological states providing insight into its contribution to disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed : 11018012). Can stimulate the ATPase activity of the catalytic subunit of these complexes (PubMed : 10078207). May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells (PubMed : 12192000). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Critical regulator of myeloid differentiation, controlling granulocytopoiesis and the expression of genes involved in neutrophil granule formation (By similarity).
See full target information SMARCC2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 24: PubMed34080022

2021

SMARCC2 combined with c‑Myc inhibits the migration and invasion of glioma cells via modulation of the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyang Li,Chengshuo Fei,Junjie Li,Hang Wu,Lei Chen,Ramzi Roshani,Hong Li,Linyong Shi,Chong Song,Junwei Gu,Yuntao Lu,Qiang Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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AB264170

Anti-BAF170 antibody

primary-antibodies

baf170-antibody-ab264170

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