Rabbit Polyclonal ACL6A antibody. Suitable for ChIP, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Images
Publications
- Nature communications 14:41932023ACTL6A protects gastric cancer cells against ferroptosis through induction of glutathione synthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqing Yang,Shaomin Zou,Yijing Zhang,Jieping Zhang,Peng Zhang,Lishi Xiao,Yunling Xie,Manqi Meng,Junyan Feng,Liang Kang,Mong-Hong Lee,Lekun FangPubMed 37443154
- eLife 9:2020Integrated analysis of H2A.Z isoforms function reveals a complex interplay in gene regulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAssala Lamaa et. al.PubMed 32109204
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ChIP | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
ChIP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Target data
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.
Alternative names
Actin-like protein 6A, 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, BRG1-associated factor 53A, INO80 complex subunit K, BAF53A, BAF53, BAF53A, ACTL6A, INO80K
Recommended products
Rabbit Polyclonal ACL6A antibody. Suitable for ChIP, WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.
Alternative names
Actin-like protein 6A, 53 kDa BRG1-associated factor A, Actin-related protein Baf53a, ArpNbeta, BRG1-associated factor 53A, INO80 complex subunit K, BAF53A, BAF53, BAF53A, ACTL6A, INO80K
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
In Western blot, ab3882 detects BAF53 and Tip60-TAP in total extracts of Tip60-TAP. Following TEV elution of Tip60-TAP ab3882 only detects BAF53. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. From July 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
The protein BAF53A also known as ACTL6A plays an important role in gene expression through chromatin remodeling. It is a subunit of the SWI/SNF-like BAF complex that is responsible for altering chromatin structure making it easier for transcription factors to access DNA. BAF53A has a molecular mass of approximately 53 kDa. It is highly expressed in neurons and plays an essential role in neurodevelopment and the regulation of neuronal gene expression.
- Biological function summary
BAF53A is an integral component of the BAF chromatin remodeling complex. This complex modifies the positioning and accessibility of nucleosomes on chromatin thereby influencing gene transcription processes. BAF53A contributes to the regulation of genes involved in neuronal differentiation and development. The protein plays a significant role in neural stem cell proliferation and the regulation of progenitor cell fate.
- Pathways
The BAF53A protein participates actively in the neural development and chromatin remodeling pathways. It interacts closely with the BAF complex to regulate the transcription of genes essential for neural differentiation and development. The BAF complex which includes BAF53A associates with proteins such as BRG1 and SMARCC1 facilitating the unfolding of chromatin to affect gene expression positively.
- Associated diseases and disorders
BAF53A is associated with neurodevelopmental disorders and certain types of cancer. Altered expression or mutations in BAF53A have been linked to neurodevelopmental issues contributing to conditions such as Autism Spectrum Disorders. Additionally overexpression of BAF53A connects to cancer particularly in glioblastoma where it influences tumor progression. In these contexts BAF53A interacts with oncogenes and tumor suppressor proteins playing a role in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
This image is courtesy of Sylvain DaujatChIP - Anti-BAF53A antibody - ChIP Grade (ab3882)
Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab3882 to BAF53A and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 8 μl of ab3882 and 2x106 cells were used in each ChIP experiment.
This image is courtesy of Jacques Cote, Universite Laval, Quebec, CanadaWestern blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
Total extracts from two different clones of MCF7 (Human breast adenocarcinoma cell line) cells expressing a TAP tagged version of Tip60 were partially purified over IgG sepharose resin and eluted with TEV protease. Western blot was performed using a 1/1000 dilution of the BAF53A antibody.
Lane 1. TEV elution of Tip60-TAP clone #1
Lane 2. TEV elution of Tip60-TAP clone #2
Lane 3. Total extract of Tip60-TAP clone #1
Lane 4. Total extract of Tip60-TAP clone #2NB: Tip60 is detected in the total extract due to the presence of Protein A in the TAP Tag (to which the primary and secondary abs bind). The TEV protease removes the Tag in lanes 1 and 2.
All lanes: Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882) at 1/1000 dilution
Lane 1: TEV elution of Tip60-TAP clone 1
Lane 2: TEV elution of Tip60-TAP clone 2
Lane 3: Total extract of Tip60-TAP clone 1
Lane 4: Total extract of Tip60-TAP clone 2
Performed under reducing conditions.
Predicted band size: 47 kDa
Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882)
All lanes: Western blot - Anti-BAF53A antibody - ChIP Grade (ab3882) at 1 µg/mL
Lane 1: Western blot - NIH/3T3 whole cell lysate (NIH/3T3 whole cell lysate ab7179) at 10 µg
Lane 2: MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Lane 3: Western blot - Mouse skeletal muscle tissue lysate - total protein (Mouse skeletal muscle tissue lysate - total protein ab29711) at 10 µg
Lane 4: Ovary (Mouse) Tissue Lysate - normal tissue at 10 µg
Lane 5: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 120 kDa, 18 kDa, 47 kDa
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