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Rabbit Monoclonal BAF53b antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.

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Images

Western blot - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex), as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity). Essential for neuronal maturation and dendrite development (PubMed:31031012).

Alternative names

ACTL6, BAF53B, ACTL6B, Actin-like protein 6B, 53 kDa BRG1-associated factor B, Actin-related protein Baf53b, ArpNalpha, BRG1-associated factor 53B

Recommended products

Rabbit Monoclonal BAF53b antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP10101
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab284832 is the carrier-free version of Anti-BAF53b antibody [EP10101] ab180927

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BAF53b also known as Actin-like protein 6B is a part of the SWI/SNF chromatin remodeling complex. It has a molecular mass of roughly 50 kDa. You can find BAF53b mostly in the brain with higher expression levels in the central nervous system during development. It primarily associates with the multi-subunit complexes in neurons indicating its specialized role in neural functions.

Biological function summary

BAF53b acts as an important player in regulating neuronal gene expression. It is part of the neuronal-specific BAF (nBAF) complex which contributes to the modification of chromatin structure. BAF53b has a specialization in managing chromatin accessibility during neural differentiation and synaptic development. Its interaction with other subunits in the SWI/SNF complex aids in the transcriptional activation of genes important for synaptic plasticity and memory formation.

Pathways

BAF53b plays significant roles in important cellular processes such as the Wnt signaling pathway and the transcription regulation pathway. In the Wnt pathway BAF53b collaborates with proteins such as β-catenin to influence gene transcription impacting cell division and neuronal differentiation. Its involvement in transcription regulation brings BAF53b into interaction with other remodeling proteins which together facilitate epigenetic changes needed for neuronal maturity.

Associated diseases and disorders

Alterations in BAF53b function relate closely with neurological conditions like schizophrenia and intellectual disabilities. Abnormalities in the BAF53b-protein complexes can disrupt synaptic functions and neuronal connectivity seen in schizophrenia. BAF53b's significant association with other proteins in neural pathways points to potential roles in regulating synaptic development and maintaining neural integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832), expandable thumbnail

    Western blot - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832)

    This data was developed using Anti-BAF53b antibody [EP10101] ab180927, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-BAF53b antibody [EP10101] (Anti-BAF53b antibody [EP10101] ab180927) at 1/5000 dilution

    All lanes: Human fetal brain lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832)

    This data was developed using Anti-BAF53b antibody [EP10101] ab180927, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse brain tissue, labeling BAF53b with Anti-BAF53b antibody [EP10101] ab180927 at 1/500 dilution and counter-stained with Hematoxylin. 

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832)

    This data was developed using Anti-BAF53b antibody [EP10101] ab180927, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin -embedded Human brain tissue, labeling BAF53b with Anti-BAF53b antibody [EP10101] ab180927 at 1/500 dilution and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EP10101] - BSA and Azide free (ab284832)

    This data was developed using Anti-BAF53b antibody [EP10101] ab180927, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U87-MG cells (paraformaldehyde-fixed, 4%) labeling BAF53b with Anti-BAF53b antibody [EP10101] ab180927 at 1//50 dillution followed by Goat anti Rabbit IgG secondary antibody at 1/200 dilution and counter-stained with Dapi (blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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