AB284832

Anti-BAF53b antibody [EP10101] - BSA and Azide free

RabMAb
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Rabbit Monoclonal BAF53b antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples.

View Alternative Names

ACTL6, BAF53B, ACTL6B, Actin-like protein 6B, 53 kDa BRG1-associated factor B, Actin-related protein Baf53b, ArpNalpha, BRG1-associated factor 53B

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

This data was developed using ab180927, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U87-MG cells (paraformaldehyde-fixed, 4%) labeling BAF53b with ab180927 at 1//50 dillution followed by Goat anti Rabbit IgG secondary antibody at 1/200 dilution and counter-stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

This data was developed using ab180927, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin -embedded Human brain tissue, labeling BAF53b with ab180927 at 1/500 dilution and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

This data was developed using ab180927, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse brain tissue, labeling BAF53b with ab180927 at 1/500 dilution and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

This data was developed using ab180927, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BAF53b antibody [EP10101] (<a href='/en-us/products/primary-antibodies/baf53b-antibody-ep10101-ab180927'>ab180927</a>) at 1/5000 dilution

All lanes:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L) HRP at 1/1000 dilution

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP10101

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab284832 is the carrier-free version of ab180927

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAF53b also known as Actin-like protein 6B is a part of the SWI/SNF chromatin remodeling complex. It has a molecular mass of roughly 50 kDa. You can find BAF53b mostly in the brain with higher expression levels in the central nervous system during development. It primarily associates with the multi-subunit complexes in neurons indicating its specialized role in neural functions.

Biological function summary

BAF53b acts as an important player in regulating neuronal gene expression. It is part of the neuronal-specific BAF (nBAF) complex which contributes to the modification of chromatin structure. BAF53b has a specialization in managing chromatin accessibility during neural differentiation and synaptic development. Its interaction with other subunits in the SWI/SNF complex aids in the transcriptional activation of genes important for synaptic plasticity and memory formation.

Pathways

BAF53b plays significant roles in important cellular processes such as the Wnt signaling pathway and the transcription regulation pathway. In the Wnt pathway BAF53b collaborates with proteins such as β-catenin to influence gene transcription impacting cell division and neuronal differentiation. Its involvement in transcription regulation brings BAF53b into interaction with other remodeling proteins which together facilitate epigenetic changes needed for neuronal maturity.

Alterations in BAF53b function relate closely with neurological conditions like schizophrenia and intellectual disabilities. Abnormalities in the BAF53b-protein complexes can disrupt synaptic functions and neuronal connectivity seen in schizophrenia. BAF53b's significant association with other proteins in neural pathways points to potential roles in regulating synaptic development and maintaining neural integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex), as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity). Essential for neuronal maturation and dendrite development (PubMed : 31031012).

See full target information ACTL6B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BAF53b antibody [EP10101] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

Western blot - Anti-BAF53b antibody [EP10101] - BSA and Azide free (AB284832)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise