Skip to main content

Rabbit Recombinant Monoclonal BAF53b antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.


Images

Western blot - Anti-BAF53b antibody [EPR10100(B)] (AB140642), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EPR10100(B)] (AB140642), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Mouse
Expected
Not recommended
Predicted
Rat
Expected
Not recommended
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/250 - 1/500

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neuron-specific chromatin remodeling complex (nBAF complex), as such plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. ACTL6B/BAF53B is not essential for assembly of the nBAF complex but is required for targeting the complex and CREST to the promoter of genes essential for dendritic growth (By similarity). Essential for neuronal maturation and dendrite development (PubMed:31031012).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal BAF53b antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR10100(B)

Purification technique

Affinity purification Protein A

Specificity

ab140642 is likely to cross-react with the homologue ACTL6A protein, due to high homology with the immunogen sequence.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

BAF53b also known as Actin-like protein 6B is a part of the SWI/SNF chromatin remodeling complex. It has a molecular mass of roughly 50 kDa. You can find BAF53b mostly in the brain with higher expression levels in the central nervous system during development. It primarily associates with the multi-subunit complexes in neurons indicating its specialized role in neural functions.

Biological function summary

BAF53b acts as an important player in regulating neuronal gene expression. It is part of the neuronal-specific BAF (nBAF) complex which contributes to the modification of chromatin structure. BAF53b has a specialization in managing chromatin accessibility during neural differentiation and synaptic development. Its interaction with other subunits in the SWI/SNF complex aids in the transcriptional activation of genes important for synaptic plasticity and memory formation.

Pathways

BAF53b plays significant roles in important cellular processes such as the Wnt signaling pathway and the transcription regulation pathway. In the Wnt pathway BAF53b collaborates with proteins such as β-catenin to influence gene transcription impacting cell division and neuronal differentiation. Its involvement in transcription regulation brings BAF53b into interaction with other remodeling proteins which together facilitate epigenetic changes needed for neuronal maturity.

Associated diseases and disorders

Alterations in BAF53b function relate closely with neurological conditions like schizophrenia and intellectual disabilities. Abnormalities in the BAF53b-protein complexes can disrupt synaptic functions and neuronal connectivity seen in schizophrenia. BAF53b's significant association with other proteins in neural pathways points to potential roles in regulating synaptic development and maintaining neural integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-BAF53b antibody [EPR10100(B)] (ab140642), expandable thumbnail

    Western blot - Anti-BAF53b antibody [EPR10100(B)] (ab140642)

    All lanes: Western blot - Anti-BAF53b antibody [EPR10100(B)] (ab140642) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: HepG2 cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Lane 4: U-87 MG cell lysate at 10 µg

    Secondary

    All lanes: HRP-labelled Goat anti-Rabbit at 1/2000 dilution

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EPR10100(B)] (ab140642), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BAF53b antibody [EPR10100(B)] (ab140642)

    Immunofluorescent analysis of HepG2 cells labelling BAF53b with ab140642 at 1/250 dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com