AB70540

Anti-BAF57/SMARCE1 antibody

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(20 Publications)

Rabbit Polyclonal BAF57/SMARCE1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 20 publications.

View Alternative Names

BAF57, SMARCE1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (AB70540)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (AB70540)

IHC image of ab70540 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70540, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (AB70540)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BAF57/SMARCE1 with ab70540 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody (AB70540)

1mg of lysate from HeLa cells was immunoprecipitated using ab70540 at 3ug/mg of lysate (lane 1 or a control rabbit IgG (lane 2). For the subsequent western blot, 20% of immunoprecipitate was loaded per lane, and ab70540 was used at 1ug/ml.

All lanes:

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody (ab70540)

Predicted band size: 47 kDa

false

Supplier Data

Western blot - Anti-BAF57/SMARCE1 antibody (AB70540)

All lanes:

Western blot - Anti-BAF57/SMARCE1 antibody (ab70540) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 5 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

293T cells at 50 µg

Lane 5:

NIH3T3 cells at 50 µg

Predicted band size: 47 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAF57 also known as SMARCE1 is a component of the SWI/SNF chromatin-remodeling complex. It aids in regulating the structure of chromatin influencing gene expression. BAF57 weighs approximately 53 kDa and is expressed widely across various tissue types with notable presence in the brain and reproductive organs. This protein plays an important role in reshaping chromatin making it essential for modulating the accessibility of transcription factors to DNA.

Biological function summary

As a part of the SWI/SNF complex BAF57/SMARCE1 facilitates the remodeling of chromatin impacting transcription regulation. By interacting directly with DNA BAF57 allows chromatin sliding and repositioning therefore influencing gene transcription. It plays a significant role in embryonic development neural progenitor cell proliferation and the regulation of cell cycle and differentiation processes. The protein helps modulate responses to hormonal and growth factor signals which are integral for proper cellular control.

Pathways

BAF57/SMARCE1 functions mainly within the WNT signaling and the nuclear hormone receptor pathways. It initiates changes in chromatin architecture impacting gene expression in pathways including those controlling cellular differentiation and development. Proteins like SMARCA4 and ARID1A often work alongside BAF57 in these pathways helping to modulate signal transduction processes that are important for cellular outcomes.

BAF57/SMARCE1 has links to certain cancers and Coffin-Siris syndrome. Mutations in this protein or alterations in its expression can disrupt normal SWI/SNF complex function leading to abnormal cell growth and division contributing to tumor development. Additionally genetic deficits in BAF57 have associated with neural development disorders particularly through the altered function of related proteins like SMARCB1 and SMARCA4 further elucidating its role in various pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.

See full target information SMARCE1

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:5800 PubMed31863007

2019

PBRM1 acts as a p53 lysine-acetylation reader to suppress renal tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Weijia Cai,Liya Su,Lili Liao,Zongzhi Z Liu,Lauren Langbein,Essel Dulaimi,Joseph R Testa,Robert G Uzzo,Zhijiu Zhong,Wei Jiang,Qin Yan,Qing Zhang,Haifeng Yang

Nature communications 10:1881 PubMed31015438

2019

BRD9 defines a SWI/SNF sub-complex and constitutes a specific vulnerability in malignant rhabdoid tumors.

Applications

Species

Unspecified reactive species

Xiaofeng Wang,Su Wang,Emma C Troisi,Thomas P Howard,Jeffrey R Haswell,Bennett K Wolf,William H Hawk,Pilar Ramos,Elaine M Oberlick,Evgeni P Tzvetkov,Aaron Ross,Francisca Vazquez,William C Hahn,Peter J Park,Charles W M Roberts

eLife 8: PubMed30860482

2019

Renal medullary carcinomas depend upon loss and are sensitive to proteasome inhibition.

Applications

Species

Unspecified reactive species

Andrew L Hong,Yuen-Yi Tseng,Jeremiah A Wala,Won-Jun Kim,Bryan D Kynnap,Mihir B Doshi,Guillaume Kugener,Gabriel J Sandoval,Thomas P Howard,Ji Li,Xiaoping Yang,Michelle Tillgren,Mahmhoud Ghandi,Abeer Sayeed,Rebecca Deasy,Abigail Ward,Brian McSteen,Katherine M Labella,Paula Keskula,Adam Tracy,Cora Connor,Catherine M Clinton,Alanna J Church,Brian D Crompton,Katherine A Janeway,Barbara Van Hare,David Sandak,Ole Gjoerup,Pratiti Bandopadhayay,Paul A Clemons,Stuart L Schreiber,David E Root,Prafulla C Gokhale,Susan N Chi,Elizabeth A Mullen,Charles Wm Roberts,Cigall Kadoch,Rameen Beroukhim,Keith L Ligon,Jesse S Boehm,William C Hahn

Nature communications 9:5139 PubMed30510198

2018

A non-canonical BRD9-containing BAF chromatin remodeling complex regulates naive pluripotency in mouse embryonic stem cells.

Applications

IP, WB

Species

Human, Human

Jovylyn Gatchalian,Shivani Malik,Josephine Ho,Dong-Sung Lee,Timothy W R Kelso,Maxim N Shokhirev,Jesse R Dixon,Diana C Hargreaves

Cell systems 6:555-568.e7 PubMed29778836

2018

Interrogation of Mammalian Protein Complex Structure, Function, and Membership Using Genome-Scale Fitness Screens.

Applications

Species

Unspecified reactive species

Joshua Pan,Robin M Meyers,Brittany C Michel,Nazar Mashtalir,Ann E Sizemore,Jonathan N Wells,Seth H Cassel,Francisca Vazquez,Barbara A Weir,William C Hahn,Joseph A Marsh,Aviad Tsherniak,Cigall Kadoch

eLife 6: PubMed28967863

2017

Chromatin accessibility underlies synthetic lethality of SWI/SNF subunits in ARID1A-mutant cancers.

Applications

Species

Unspecified reactive species

Timothy W R Kelso,Devin K Porter,Maria Luisa Amaral,Maxim N Shokhirev,Christopher Benner,Diana C Hargreaves

Proceedings of the National Academy of Sciences of 114:4153-4158 PubMed28377514

2017

SMARCE1 is required for the invasive progression of in situ cancers.

Applications

ChIP, WB, IHC-P, IP

Species

Human, Human, Human, Human

Ethan S Sokol,Yu-Xiong Feng,Dexter X Jin,Minu D Tizabi,Daniel H Miller,Malkiel A Cohen,Sandhya Sanduja,Ferenc Reinhardt,Jai Pandey,Daphne A Superville,Rudolf Jaenisch,Piyush B Gupta

Nature genetics 49:213-222 PubMed27941796

2016

Dynamics of BAF-Polycomb complex opposition on heterochromatin in normal and oncogenic states.

Applications

Species

Mouse

Cigall Kadoch,Robert T Williams,Joseph P Calarco,Erik L Miller,Christopher M Weber,Simon M G Braun,John L Pulice,Emma J Chory,Gerald R Crabtree

Nature genetics 49:296-302 PubMed27941798

2016

ARID1A loss impairs enhancer-mediated gene regulation and drives colon cancer in mice.

Applications

Species

Human

Radhika Mathur,Burak H Alver,Adrianna K San Roman,Boris G Wilson,Xiaofeng Wang,Agoston T Agoston,Peter J Park,Ramesh A Shivdasani,Charles W M Roberts

Nature genetics 49:289-295 PubMed27941797

2016

SMARCB1-mediated SWI/SNF complex function is essential for enhancer regulation.

Applications

IP, MS

Species

Unspecified reactive species, Unspecified reactive species

Xiaofeng Wang,Ryan S Lee,Burak H Alver,Jeffrey R Haswell,Su Wang,Jakub Mieczkowski,Yotam Drier,Shawn M Gillespie,Tenley C Archer,Jennifer N Wu,Evgeni P Tzvetkov,Emma C Troisi,Scott L Pomeroy,Jaclyn A Biegel,Michael Y Tolstorukov,Bradley E Bernstein,Peter J Park,Charles W M Roberts

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BAF57/SMARCE1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (AB70540)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody (AB70540)

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody (AB70540)

Western blot - Anti-BAF57/SMARCE1 antibody (AB70540)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (20)

Product promise