AB137081

Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal BAF57/SMARCE1 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

BAF57, SMARCE1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Immunofluorescent analysis of MCF7 cells labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BAF57/SMARCE1 with purified ab137081 at 1/100 dilution (10.38μg/mL) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlabeled control - Unlabelled cells (blue).

Lab

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

ab137081 (purified) at 1/20 dilution immunoprecipitating BAF57/SMARCE1 in MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg.

Lane 1 (input) : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2 (+) : ab137081 & MCF7 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab137081 in MCF7 whole cell lysate

For western blotting, ab137081 at 1/500 dilution (0.02 μg/mL) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution was used.

Blocking and diluting buffer : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

Predicted band size: 47 kDa

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ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

ChIP

Unknown

ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab137081 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
*http : //www.abcam.com/resources?keywords=X%20ChIP%20protocol

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Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

All lanes:

Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081) at 1/1000 dilution

Lane 1:

MCF7 cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

Raji cell lysates at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

false

Carrier free

Anti-BAF57/SMARCE1 antibody [EPR8849] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8849

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ChIP, IP, Flow Cyt (Intra), WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAF57 also known as SMARCE1 is a component of the SWI/SNF chromatin-remodeling complex. It aids in regulating the structure of chromatin influencing gene expression. BAF57 weighs approximately 53 kDa and is expressed widely across various tissue types with notable presence in the brain and reproductive organs. This protein plays an important role in reshaping chromatin making it essential for modulating the accessibility of transcription factors to DNA.

Biological function summary

As a part of the SWI/SNF complex BAF57/SMARCE1 facilitates the remodeling of chromatin impacting transcription regulation. By interacting directly with DNA BAF57 allows chromatin sliding and repositioning therefore influencing gene transcription. It plays a significant role in embryonic development neural progenitor cell proliferation and the regulation of cell cycle and differentiation processes. The protein helps modulate responses to hormonal and growth factor signals which are integral for proper cellular control.

Pathways

BAF57/SMARCE1 functions mainly within the WNT signaling and the nuclear hormone receptor pathways. It initiates changes in chromatin architecture impacting gene expression in pathways including those controlling cellular differentiation and development. Proteins like SMARCA4 and ARID1A often work alongside BAF57 in these pathways helping to modulate signal transduction processes that are important for cellular outcomes.

BAF57/SMARCE1 has links to certain cancers and Coffin-Siris syndrome. Mutations in this protein or alterations in its expression can disrupt normal SWI/SNF complex function leading to abnormal cell growth and division contributing to tumor development. Additionally genetic deficits in BAF57 have associated with neural development disorders particularly through the altered function of related proteins like SMARCB1 and SMARCA4 further elucidating its role in various pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.

See full target information SMARCE1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9614 PubMed39511190

2024

The BAF complex enhances transcription through interaction with H3K56ac in the histone globular domain.

Applications

Unspecified application

Species

Unspecified reactive species

Kwangbeom Hyun,Jihye Ahn,Hyoungmin Kim,Jihyun Kim,Yong-In Kim,Hee-Sung Park,Robert G Roeder,J Eugene Lee,Jaehoon Kim

Nature 607:135-141 PubMed35732731

2022

cBAF complex components and MYC cooperate early in CD8 T cell fate.

Applications

Unspecified application

Species

Unspecified reactive species

Ao Guo,Hongling Huang,Zhexin Zhu,Mark J Chen,Hao Shi,Sujing Yuan,Piyush Sharma,Jon P Connelly,Swantje Liedmann,Yogesh Dhungana,Zhenrui Li,Dalia Haydar,Mao Yang,Helen Beere,Jason T Yustein,Christopher DeRenzo,Shondra M Pruett-Miller,Jeremy Chase Crawford,Giedre Krenciute,Charles W M Roberts,Hongbo Chi,Douglas R Green

eLife 11: PubMed35551737

2022

Inositol polyphosphate multikinase physically binds to the SWI/SNF complex and modulates BRG1 occupancy in mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jiyoon Beon,Sungwook Han,Hyeokjun Yang,Seung Eun Park,Kwangbeom Hyun,Song-Yi Lee,Hyun-Woo Rhee,Jeong Kon Seo,Jaehoon Kim,Seyun Kim,Daeyoup Lee

Nature genetics 51:1399-1410 PubMed31427792

2019

Systematic characterization of BAF mutations provides insights into intracomplex synthetic lethalities in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Schick,André F Rendeiro,Kathrin Runggatscher,Anna Ringler,Bernd Boidol,Melanie Hinkel,Peter Májek,Loan Vulliard,Thomas Penz,Katja Parapatics,Christian Schmidl,Jörg Menche,Guido Boehmelt,Mark Petronczki,André C Müller,Christoph Bock,Stefan Kubicek

Molecular reproduction and development 84:1238-1249 PubMed29024220

2017

Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo.

Applications

ICC/IF

Species

Unspecified reactive species

Birgit Cabot,Yu-Chun Tseng,Jennifer S Crodian,Ryan Cabot

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade

Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Flow Cytometry (Intracellular) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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