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Rabbit Recombinant Monoclonal BAF57/SMARCE1 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

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Images

Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081), expandable thumbnail
  • ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081), expandable thumbnail
  • Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (AB137081), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPChIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Required for the coactivation of estrogen responsive promoters by SWI/SNF complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells.

Alternative names

BAF57, SMARCE1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57

Recommended products

Rabbit Recombinant Monoclonal BAF57/SMARCE1 antibody. Suitable for IHC-P, IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8849
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BAF57 also known as SMARCE1 is a component of the SWI/SNF chromatin-remodeling complex. It aids in regulating the structure of chromatin influencing gene expression. BAF57 weighs approximately 53 kDa and is expressed widely across various tissue types with notable presence in the brain and reproductive organs. This protein plays an important role in reshaping chromatin making it essential for modulating the accessibility of transcription factors to DNA.

Biological function summary

As a part of the SWI/SNF complex BAF57/SMARCE1 facilitates the remodeling of chromatin impacting transcription regulation. By interacting directly with DNA BAF57 allows chromatin sliding and repositioning therefore influencing gene transcription. It plays a significant role in embryonic development neural progenitor cell proliferation and the regulation of cell cycle and differentiation processes. The protein helps modulate responses to hormonal and growth factor signals which are integral for proper cellular control.

Pathways

BAF57/SMARCE1 functions mainly within the WNT signaling and the nuclear hormone receptor pathways. It initiates changes in chromatin architecture impacting gene expression in pathways including those controlling cellular differentiation and development. Proteins like SMARCA4 and ARID1A often work alongside BAF57 in these pathways helping to modulate signal transduction processes that are important for cellular outcomes.

Associated diseases and disorders

BAF57/SMARCE1 has links to certain cancers and Coffin-Siris syndrome. Mutations in this protein or alterations in its expression can disrupt normal SWI/SNF complex function leading to abnormal cell growth and division contributing to tumor development. Additionally genetic deficits in BAF57 have associated with neural development disorders particularly through the altered function of related proteins like SMARCB1 and SMARCA4 further elucidating its role in various pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081), expandable thumbnail

    Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    ab137081 (purified) at 1/20 dilution immunoprecipitating BAF57/SMARCE1 in MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg.

    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg
    Lane 2 (+): ab137081 & MCF7 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab137081 in MCF7 whole cell lysate

    For western blotting, ab137081 at 1/500 dilution (0.02 μg/mL) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Predicted band size: 47 kDa

  • ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081), expandable thumbnail

    ChIP - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Chromatin was prepared from HeLa cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab137081 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081), expandable thumbnail

    Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    All lanes: Western blot - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081) at 1/1000 dilution

    Lane 1: MCF7 cell lysates at 10 µg

    Lane 2: HeLa cell lysates at 10 µg

    Lane 3: Jurkat cell lysates at 10 µg

    Lane 4: Raji cell lysates at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Flow Cytometry (Intracellular) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Intracellular Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BAF57/SMARCE1 with purified ab137081 at 1/100 dilution (10.38μg/mL) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlabeled control - Unlabelled cells (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BAF57/SMARCE1 antibody [EPR8849] - ChIP Grade (ab137081)

    Immunofluorescent analysis of MCF7 cells labelling BAF57/SMARCE1 with ab137081 at 1/250 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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