JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB8396

Anti-BAFF antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal BAFF antibody. Suitable for WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TNFSF13B aa 250 to C-terminus.

View Alternative Names

CD257, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, TNFSF13B, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, BLyS, TALL-1

1 Images
Western blot - Anti-BAFF antibody (AB8396)
  • WB

Unknown

Western blot - Anti-BAFF antibody (AB8396)

All lanes:

Western blot - Anti-BAFF antibody (ab8396) at 1 µg/mL

Lane 1:

Human HL60 cell lysate

Lane 2:

Murine spleen tissue lysate

Predicted band size: 31 kDa

Observed band size: 34 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Light chain type

unknown

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human TNFSF13B aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y275

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.25-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.
Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.. Isoform 3. Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.
See full target information TNFSF13B
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Cell physiology 326:C1494-C1504 PubMed38406824

2024

Implications of IFNγ SNP rs2069705 in primary Sjögren's syndrome: transcriptional activation and B cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Min Li,Honglin Li,Miao Liu,Jianrong Su,Yuzhu Ji

Molecular medicine reports 21:795-805 PubMed31974601

2020

BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF‑κB signaling pathway in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Cao,Guoyuan Lu,Xiaolan Chen,Xu Chen,Naifeng Guo,Wenwen Li

International journal of pediatric otorhinolaryngology 80:49-52 PubMed26746612

2016

Characterization of B-Cells in tonsils of patients diagnosed with pediatric autoimmune neuropsychiatric disorder associated streptococcus.

Applications

WB

Species

Human

Andrew Walls,Sarah Dermody,Ravindran Kumaran,Nathan Krishnan,Earl H Harley

Molecular immunology 53:15-23 PubMed22784990

2012

Murine BAFF expression is up-regulated by estrogen and interferons: implications for sex bias in the development of autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Ravichandran Panchanathan,Divaker Choubey
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB245833

Anti-BAFF antibody [EPR22238] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry - Anti-BAFF antibody [EPR22238] - BSA and Azide free (AB245833)

  • 0
  • 0
Primary Antibodies

AB203791

Anti-BAFF antibody [EPR19704]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (AB203791)

  • 0
  • 0
Primary Antibodies

AB217329

Anti-BAFF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF antibody (AB217329)

  • 0
  • 0
Primary Antibodies

AB224710

Anti-BAFF antibody [EPR22238]

RabMAb|Recombinant

Flow Cytometry - Anti-BAFF antibody [EPR22238] (AB224710)

  • 1
  • 1

Complementary Products

Assay Kits

AB188391

Human BAFF ELISA Kit (TNFSF13B)

SimpleStep

Sandwich ELISA - Human BAFF ELISA Kit (TNFSF13B) (AB188391)

  • 0
  • 0
Primary Antibodies

AB5965

Anti-BAFF-R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (AB5965)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB10558

Anti-CD45 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody (AB10558)

  • 4
  • 61
Primary Antibodies

AB108206

Anti-APRIL/TNFSF13 antibody [EPR4286]

RabMAb|Recombinant

Western blot - Anti-APRIL/TNFSF13 antibody [EPR4286] (AB108206)

  • 0
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com