AB203791

Anti-BAFF antibody [EPR19704]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal BAFF antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD257, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, TNFSF13B, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, BLyS, TALL-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (AB203791)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (AB203791)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized 293T (Human epithelial cell line from embryonic kidney) cells labeling BAFF with ab203791 at 1/100 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 647) (ab150079) secondary antibody at 1/1000 dilution.

Confocal image showing positive staining on 293T cells transfected with a GFP-tagged BAFF expression construct.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is ab150079 at 1/1000 dilution.

ICC/IF was also performed on a few cell lines including U937 no signal was detected.

Supplier Data

Immunoprecipitation - Anti-BAFF antibody [EPR19704] (AB203791)

BAFF was immunoprecipitated from 0.35 mg of U937 (Human histiocytic lymphoma cell line) whole cell lysate with ab203791 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab203791 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : U937 whole cell lysate, 10μg (Input).

Lane 2 : ab203791 IP in U937 whole cell lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab203791 in U937 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-BAFF antibody [EPR19704] (ab203791)

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-BAFF antibody [EPR19704] (AB203791)

Blocking/Dilution buffer : 5% NFDM/TBST.

Two isoforms have been described in literature (PMID : 12867412).

All lanes:

Western blot - Anti-BAFF antibody [EPR19704] (ab203791) at 1/1000 dilution

All lanes:

U937 (Human histiocytic lymphoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 17 kDa,29 kDa,31 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-BAFF antibody [EPR19704] (AB203791)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BAFF antibody [EPR19704] (ab203791) at 1/5000 dilution

Lane 1:

Empty vector with GFP-Myc tag (vector control) transfected HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 10 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) transfected with a GFP-Myc tagged BAFF vector whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 31 kDa

Observed band size: 57 kDa

false

Exposure time: 1s

Carrier free

Anti-BAFF antibody [EPR19704] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19704

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.

Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.. Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.. Isoform 3. Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.

See full target information TNFSF13B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmaceutica Sinica. B 13:4273-4290 PubMed37799388

2023

Use of recombinant microRNAs as antimetabolites to inhibit human non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yixin Chen,Mei-Juan Tu,Fangwei Han,Zhenzhen Liu,Neelu Batra,Primo N Lara,Hong-Wu Chen,Huichang Bi,Ai-Ming Yu

Journal of translational medicine 21:466 PubMed37443022

2023

A precise molecular subtyping of ulcerative colitis reveals the immune heterogeneity and predicts clinical drug responses.

Applications

Unspecified application

Species

Unspecified reactive species

Shaocong Mo,Bryan Jin,Yujen Tseng,Lingxi Lin,Lishuang Lin,Xin Shen,Huan Song,Mingjia Kong,Zhongguang Luo,Yiwei Chu,Chen Jiang,Zhiwei Cao,Jie Liu,Feifei Luo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BAFF antibody [EPR19704]

Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR19704] (AB203791)

Immunoprecipitation - Anti-BAFF antibody [EPR19704] (AB203791)

Western blot - Anti-BAFF antibody [EPR19704] (AB203791)

Western blot - Anti-BAFF antibody [EPR19704] (AB203791)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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