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Rabbit Recombinant Monoclonal BAFF antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.


Images

Immunoprecipitation - Anti-BAFF antibody [EPR22238] (AB224710), expandable thumbnail
  • Flow Cytometry - Anti-BAFF antibody [EPR22238] (AB224710), expandable thumbnail
  • Western blot - Anti-BAFF antibody [EPR22238] (AB224710), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (AB224710), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info
1/30
Notes

-

Tested
Tested

Species

Human

Dilution info
1/500
Notes

-

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.Isoform 2 seems to inhibit isoform 1 secretion and bioactivity.Isoform 3Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis.

Alternative names

CD257, BAFF, BLYS, TALL1, TNFSF20, ZTNF4, UNQ401/PRO738, TNFSF13B, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, B-cell-activating factor, BAFF, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, BLyS, TALL-1

Recommended products

Rabbit Recombinant Monoclonal BAFF antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22238

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

BAFF also known as B-cell activating factor or TNFSF13B is a protein important for B-cell survival and maturation. It's a type II transmembrane protein expressed in cells like macrophages and dendritic cells and has a mass of approximately 31 kDa. The BAFF protein is also present on activated T-cells and in the serum as a soluble form. It functions as a cytokine and influences various stages of B-cell development.

Biological function summary

BAFF plays a significant role in the humoral immune response by enhancing B-cell proliferation and function. It belongs to the tumor necrosis factor (TNF) ligand family and interacts as a homotrimer. The BAFF cytokine can bind to receptors such as BAFF-R BCMA (B-cell maturation antigen) and TACI (transmembrane activator and CAML interactor). These interactions regulate B-cell homeostasis promoting development and differentiation processes important for normal immune responses.

Pathways

BAFF is central to the regulation of immune system signaling particularly in B-cell receptor (BCR) signaling and NF-kB pathway activation. The BAFF-R mediates B-cell survival signals through the NF-kB pathway while TACI and BCMA associations convey additional immune responses regulation. These multiple pathways ensure the maintenance of B-cell populations and their ability to produce antibodies.

Associated diseases and disorders

BAFF has been linked to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Elevated levels of BAFF can exacerbate autoimmune responses due to increased B-cell survival. Connections with these disorders involve the BAFF-R and its influence on excessive B-cell proliferation contributing to disease pathology. Therapeutically anti-BAFF therapies target these interactions to modify disease progression and manage autoimmunity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710), expandable thumbnail

    Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710)

    BAFF was immunoprecipitated from 0.35 mg of U937 (human histiocytic lymphoma cell line) whole cell lysate with ab224710 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224710 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: U937 whole cell lysate 10 μg (Input).

    Lane 2: ab224710 IP in U937 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab224710 in U937 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 20 seconds.

    All lanes: Immunoprecipitation - Anti-BAFF antibody [EPR22238] (ab224710)

    Predicted band size: 31 kDa

  • Flow Cytometry - Anti-BAFF antibody [EPR22238] (ab224710), expandable thumbnail

    Flow Cytometry - Anti-BAFF antibody [EPR22238] (ab224710)

    Flow cytometric analysis of Daudi (human Burkitt's lymphoma cell line; left panel) and HL-60 (human promyelocytic leukemia cell line ;right panel) cell lines labeling BAFF with ab224710 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Daudi. (PMID: 20951740)

    Gated on viable cells.

  • Western blot - Anti-BAFF antibody [EPR22238] (ab224710), expandable thumbnail

    Western blot - Anti-BAFF antibody [EPR22238] (ab224710)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-BAFF antibody [EPR22238] (ab224710) at 1/1000 dilution

    Lane 1: U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

    Lane 2: HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 20 µg

    Lane 3: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 4: Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 31 kDa

    Observed band size: 30 kDa, 32 kDa

    Exposure time: 26s

  • Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (ab224710), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BAFF antibody [EPR22238] (ab224710)

    Immunofluorescent analysis of 100% methanol-fixed U937 (human histiocytic lymphoma cell line) cells labeling BAFF with ab224710 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on U937 cells.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

    Confocal image showing membranous staining in U-937 cells.

    We don't recommend PFA fixation, since the result shows some background staining in the nuclear.

    Negative control: HEK-293（PMID: 17393395.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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