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AB233775

Anti-BAFF-R antibody [5A9B6]

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(3 Publications)

Mouse Monoclonal BAFF-R antibody. Suitable for Flow Cyt, WB and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF13C aa 1-100.

View Alternative Names

CD268, BAFFR, BR3, TNFRSF13C, Tumor necrosis factor receptor superfamily member 13C, B-cell-activating factor receptor, BAFF receptor, BLyS receptor 3, BAFF-R

4 Images
Flow Cytometry - Anti-BAFF-R antibody [5A9B6] (AB233775)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-BAFF-R antibody [5A9B6] (AB233775)

Flow cytometric analysis of Raji (human Burkitt's lymphoma cell line) cells labeling BAFF-R using ab233775 at 1/200 dilution (green) and the negative control (red).

Western blot - Anti-BAFF-R antibody [5A9B6] (AB233775)
  • WB

Supplier Data

Western blot - Anti-BAFF-R antibody [5A9B6] (AB233775)

Expected MW is 34 kDa.

All lanes:

Western blot - Anti-BAFF-R antibody [5A9B6] (ab233775) at 1/500 dilution

All lanes:

BAFF-R (AA: extra 1-78) human recombinant protein

Predicted band size: 19 kDa

false

Western blot - Anti-BAFF-R antibody [5A9B6] (AB233775)
  • WB

Supplier Data

Western blot - Anti-BAFF-R antibody [5A9B6] (AB233775)

All lanes:

Western blot - Anti-BAFF-R antibody [5A9B6] (ab233775) at 1/500 dilution

All lanes:

C6 (Rat glial tumor cell line) cell lysate

Predicted band size: 19 kDa

false

Western blot - Anti-BAFF-R antibody [5A9B6] (AB233775)
  • WB

Supplier Data

Western blot - Anti-BAFF-R antibody [5A9B6] (AB233775)

All lanes:

Western blot - Anti-BAFF-R antibody [5A9B6] (ab233775) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

BAFF-R (AA: extra 1-78)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 19 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5A9B6

Isotype

IgG2b

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF13C aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96RJ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BAFF-R also known as B-cell activating factor receptor or BAFF receptor plays a critical role in B-cell development and survival. BAFF-R is a receptor for the BAFF protein also referred to as B lymphocyte stimulator (BLyS). BAFF-R has a molecular mass of approximately 19 kDa. The expression of BAFF-R is found on B cells with higher presence on mature B cells compared to immature ones. The receptor is recognized as the main signaling partner for BAFF which forms the basis for many of its immune functions.
Biological function summary

BAFF-R is essential for the maturation and maintenance of peripheral B cells. The receptor contributes to the regulation of immune responses by ensuring B-cell homeostasis. It does not generally form part of a larger protein complex but instead functions as a membrane-bound receptor that transmits signals upon binding with the BAFF ligand. This signaling cascade supports the survival and proliferation of B cells which is fundamental for the adaptive immune system.

Pathways

BAFF-R is deeply involved in the NF-κB signaling pathway which is important for immune regulation and cell survival. Upon binding with BAFF BAFF-R recruits associated proteins to initiate the pathway and as a result promotes gene transcription important for B-cell function. Another connected pathway involves AKT a protein kinase that regulates B-cell metabolism and differentiation. Both NF-κB and AKT pathways help facilitate the immune functions tied to B cells directly through BAFF-R signaling.

BAFF-R demonstrates a notable link with autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. Overexpression of BAFF impacting interaction with BAFF-R contributes to such conditions by promoting the survival of autoreactive B cells. In SLE BAFF works with another receptor TACI influencing the proliferation of autoreactive cells. The persistent activation and dysfunction of the immune response through BAFF-R interactions exacerbate these autoimmune conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
See full target information TNFRSF13C
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:2004-2016 PubMed35030978

2022

Human bone marrow mesenchymal stem cells-derived exosomes attenuated prostate cancer progression via the miR-99b-5p/IGF1R axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shichun Jiang,Haiyu Chen,Kai He,Juan Wang

Frontiers in immunology 11:563699 PubMed33324395

2020

microRNA-630 Regulates Underglycosylated IgA1 Production in the Tonsils by Targeting TLR4 in IgA Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Liu,Mu-Yao Ye,Wen-Zhe Yan,Xiao-Fei Peng,Li-Yu He,You-Ming Peng

Molecular medicine reports 21:795-805 PubMed31974601

2020

BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF‑κB signaling pathway in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Cao,Guoyuan Lu,Xiaolan Chen,Xu Chen,Naifeng Guo,Wenwen Li
View all publications
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