Rabbit Polyclonal BAFF-R antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TNFRSF13C.
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- Phytomedicine : international journal of phytotherapy and phytopharmacology 80:1533812020Artesunate inhibits Sjögren's syndrome-like autoimmune responses and BAFF-induced B cell hyperactivation via TRAF6-mediated NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianle Zhan,Baoli Wang,Jiayao Fu,Yanxiong Shao,Lei Ye,Huan Shi,Lingyan ZhengPubMed 33086170
- Orphanet journal of rare diseases 15:1812020Factors associated with refractory autoimmune necrotizing myopathy with anti-signal recognition particle antibodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYawen Zhao,Wei Zhang,Yilin Liu,Zhaoxia Wang,Yun YuanPubMed 32641139
- Molecular medicine reports 21:795-8052020BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF‑κB signaling pathway in glomerular mesangial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingjie Cao,Guoyuan Lu,Xiaolan Chen,Xu Chen,Naifeng Guo,Wenwen LiPubMed 31974601
- Journal of cellular biochemistry 120:15429-154422019microRNA-96 promotes osteoblast differentiation and bone formation in ankylosing spondylitis mice through activating the Wnt signaling pathway by binding to SOST.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Ma et. al.PubMed 31111563
- Human molecular genetics 26:3362-33742017Comparison of spinocerebellar ataxia type 3 mouse models identifies early gain-of-function, cell-autonomous transcriptional changes in oligodendrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBiswarathan Ramani,Bharat Panwar,Lauren R Moore,Bo Wang,Rogerio Huang,Yuanfang Guan,Henry L PaulsonPubMed 28854700
- The Journal of clinical investigation 123:5135-452013Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJordan V Price,David J Haddon,Dodge Kemmer,Guillaume Delepine,Gil Mandelbaum,Justin A Jarrell,Rohit Gupta,Imelda Balboni,Eliza F Chakravarty,Jeremy Sokolove,Anthony K Shum,Mark S Anderson,Mickie H Cheng,William H Robinson,Sarah K Browne,Steven M Holland,Emily C Baechler,Paul J UtzPubMed 24270423
- Neurology international 5:e42013Expression of B-cell activating factor, a proliferating inducing ligand and its receptors in primary central nervous system lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Birnbaum et. al.PubMed 23717783
- The Journal of experimental medicine 207:2569-792010A BAFF-R mutation associated with non-Hodgkin lymphoma alters TRAF recruitment and reveals new insights into BAFF-R signaling.
- Haematologica 91:772-802006The differential expression of LCK and BAFF-receptor and their role in apoptosis in human lymphomas.Applications:WB, IHC-PReactive species:HumanJennifer C Paterson,Sara Tedoldi,Andrew Craxton,Margaret Jones,Martin-Leo Hansmann,Graham Collins,Helen Roberton,Yasodha Natkunam,Stefano Pileri,Elias Campo,Edward A Clark,David Y Mason,Teresa MarafiotiPubMed 16769579
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TNFRSF13C. The exact immunogen used to generate this antibody is proprietary information. Database link Q96RJ3
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Expected | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2.00000-5.00000 µg/mL | Notes - |
Species Human | Dilution info 2.00000-5.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 2.00000-5.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.
Alternative names
CD268, BAFFR, BR3, TNFRSF13C, Tumor necrosis factor receptor superfamily member 13C, B-cell-activating factor receptor, BAFF receptor, BLyS receptor 3, BAFF-R
Recommended products
Rabbit Polyclonal BAFF-R antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TNFRSF13C.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TNFRSF13C. The exact immunogen used to generate this antibody is proprietary information. Database link Q96RJ3
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
BAFF-R Antibody is affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BAFF-R also known as B-cell activating factor receptor or BAFF receptor plays a critical role in B-cell development and survival. BAFF-R is a receptor for the BAFF protein also referred to as B lymphocyte stimulator (BLyS). BAFF-R has a molecular mass of approximately 19 kDa. The expression of BAFF-R is found on B cells with higher presence on mature B cells compared to immature ones. The receptor is recognized as the main signaling partner for BAFF which forms the basis for many of its immune functions.
- Biological function summary
BAFF-R is essential for the maturation and maintenance of peripheral B cells. The receptor contributes to the regulation of immune responses by ensuring B-cell homeostasis. It does not generally form part of a larger protein complex but instead functions as a membrane-bound receptor that transmits signals upon binding with the BAFF ligand. This signaling cascade supports the survival and proliferation of B cells which is fundamental for the adaptive immune system.
- Pathways
BAFF-R is deeply involved in the NF-κB signaling pathway which is important for immune regulation and cell survival. Upon binding with BAFF BAFF-R recruits associated proteins to initiate the pathway and as a result promotes gene transcription important for B-cell function. Another connected pathway involves AKT a protein kinase that regulates B-cell metabolism and differentiation. Both NF-κB and AKT pathways help facilitate the immune functions tied to B cells directly through BAFF-R signaling.
- Associated diseases and disorders
BAFF-R demonstrates a notable link with autoimmune diseases such as systemic lupus erythematosus (SLE) and rheumatoid arthritis. Overexpression of BAFF impacting interaction with BAFF-R contributes to such conditions by promoting the survival of autoreactive B cells. In SLE BAFF works with another receptor TACI influencing the proliferation of autoreactive cells. The persistent activation and dysfunction of the immune response through BAFF-R interactions exacerbate these autoimmune conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
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5 product images
Western blot - Anti-BAFF-R antibody (ab5965)
Western blot analysis of BAFF-R in human (H), mouse (M), and rat (R) spleen tissue lysates with ab5965 at 5 μg/ml.
Western blot analysis of BAFF-R in human (H), mouse (M), and rat (R) spleen tissue lysates with ab5965 at 5 μg/ml.
All lanes: Western blot - Anti-BAFF-R antibody (ab5965)
Predicted band size: 19 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (ab5965)
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of human spleen tissue labeling BAFF-R with Anti-BAFF-R antibody (ab5965) at 20μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (ab5965)
Immunohistochemistry of BAFF-R in human spleen tissue with BAFF-R antibody at 5 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAFF-R antibody (ab5965)
Immunohistochemistry (formalin-fixed paraffin-embedded) of BAFF-R in human tonsil tissue with ab5965 at 5 ug/ml.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-BAFF-R antibody (ab5965)
BAFF-R western blot using anti-BAFF-R antibody ab5965. Publication image and figure legend from Zhao, Y., Zhang, W., et al., 2020, Orphanet J Rare Dis, PubMed 32641139.
ab5965 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5965 please see the product overview.
Western bolt of BAFF and BAFF-R in skeletal muscle of ANM-SRP. The results showed that the expression of BAFF-R in skeletal muscle of the patients was significantly higher than that of the healthy control. BAFF were expressed in skeletal muscle of both patients and healthy control
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