Rabbit Polyclonal BAG3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAG3.
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- iScience 26:1083702023Molecular subtyping and characterization of clear cell renal cell carcinoma by tumor differentiation trajectories.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiu-Wu Pan,Wen-Jin Chen,Da Xu,Wen-Bin Guan,Lin Li,Jia-Xin Chen,Wei-Jie Chen,Ke-Qin Dong,Jian-Qing Ye,Si-Shun Gan,Wang Zhou,Xin-Gang CuiPubMed 38034348
- Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 20:524-5452023Bicalutamide and Trehalose Ameliorate Spinal and Bulbar Muscular Atrophy Pathology in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariarita Galbiati,Marco Meroni,Marina Boido,Matilde Cescon,Paola Rusmini,Valeria Crippa,Riccardo Cristofani,Margherita Piccolella,Veronica Ferrari,Barbara Tedesco,Elena Casarotto,Marta Chierichetti,Marta Cozzi,Francesco Mina,Maria Elena Cicardi,Silvia Pedretti,Nico Mitro,Anna Caretto,Patrizia Risè,Angelo Sala,Andrew P Lieberman,Paolo Bonaldo,Maria Pennuto,Alessandro Vercelli,Angelo PolettiPubMed 36717478
- Cancers 13:2021Targeting HSF1 as a Therapeutic Strategy for Multiple Mechanisms of EGFR Inhibitor Resistance in EGFR Mutant Non-Small-Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSangah Lee,Jiyae Jung,Yu-Jin Lee,Seon-Kyu Kim,Jung-Ae Kim,Bo-Kyung Kim,Kyung Chan Park,Byoung-Mog Kwon,Dong Cho HanPubMed 34203709
- Frontiers in oncology 11:6520852021Retinoic Acid Downregulates HSPB8 Gene Expression in Human Breast Cancer Cells MCF-7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargherita Piccolella,Riccardo Cristofani,Barbara Tedesco,Marta Chierichetti,Veronica Ferrari,Elena Casarotto,Marta Cozzi,Valeria Crippa,Paola Rusmini,Mariarita Galbiati,Angelo Poletti,Elio MessiPubMed 34136389
- Brain pathology (Zurich, Switzerland) 31:e129622021Inflammatory features in sporadic late-onset nemaline myopathy are independent from monoclonal gammopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJantima Tanboon,Akinori Uruha,Yukie Arahata,Carsten Dittmayer,Leonille Schweizer,Hans-Hilmar Goebel,Ichizo Nishino,Werner StenzelPubMed 34043258
- JCI insight 4:2019Investigation of a dilated cardiomyopathy-associated variant in BAG3 using genome-edited iPSC-derived cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChris McDermott-Roe et. al.PubMed 31723063
- Neurology. Genetics 5:e3492019New family with 8-associated autosomal dominant rimmed vacuolar myopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSejad Al-Tahan et. al.PubMed 31403083
- OncoTargets and therapy 12:2829-28422019FAM111B, a direct target of p53, promotes the malignant process of lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaijun Sun et. al.PubMed 31114230
- Autophagy 15:1051-10682019Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMansour Haidar et. al.PubMed 30669930
- Translational neurodegeneration 8:32019Suppression of astrocytic autophagy by αB-crystallin contributes to α-synuclein inclusion formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShen-Zhao Lu,Yong-Shun Guo,Pei-Zhou Liang,Shu-Zhen Zhang,Shu Yin,Yan-Qing Yin,Xiao-Min Wang,Fei Ding,Xiao-Song Gu,Jia-Wei ZhouPubMed 30675347
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BAG3. The exact immunogen used to generate this antibody is proprietary information. Database link O95817
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Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested | Expected |
| Mouse | Expected | Expected | Expected | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/2000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:27474739, PubMed:9873016). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).
Alternative names
BIS, BAG3, BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1
Recommended products
Rabbit Polyclonal BAG3 antibody. Suitable for IP, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 24 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAG3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: Whole serum- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BAG3. The exact immunogen used to generate this antibody is proprietary information. Database link O95817
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Bcl-2-associated athanogene 3 commonly known as Bag3 is a protein with a molecular weight of approximately 74 kDa. It plays an important role in apoptosis regulation and stress responses by interacting with heat shock proteins. Bag3 is expressed in various tissues including the heart skeletal muscles and certain types of neurons. It functions mechanically by binding to chaperones like Hsp70 modulating their activity and helping in protein folding and degradation processes.
- Biological function summary
Bag3 contributes to cell survival by assisting in protein homeostasis and mitigating stress-related cellular damage. It acts as a co-chaperone and forms part of complexes with Hsp70 HspB8 and CHIP among others. These interactions enhance the cellular capacity to handle misfolded proteins preventing the aggregation that could lead to cell toxicity. In muscle cells Bag3 plays a role in maintaining structural integrity by participating in the assembly of myofibrils critical for muscle function.
- Pathways
The protein is involved in autophagy and apoptosis regulation. It actively participates in the ubiquitin-proteasome system and chaperone-mediated autophagy collaborating closely with proteins like HspB8 and Hsp70. The integration of Bag3 within these pathways highlights its involvement in removing damaged proteins and preserving cellular function during stress conditions. Additionally Bag3 contributes to the regulation of the NF-kB pathway which is involved in the immune response and inflammation.
- Associated diseases and disorders
Mutations or dysregulation of Bag3 associate with conditions such as myopathy and certain cancers. In myopathy aberrations in Bag3 affect muscle resilience and repair leading to structural muscle defects. In cancer Bag3's role in inhibiting apoptosis promotes tumor cell survival making it a target for therapeutic interventions. The protein has connections with p53 in cancer contexts where it influences cell death and survival decisions. Understanding Bag3's interactions and functions in these diseases opens new opportunities for developing treatments targeting these pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Bag3 antibody (ab47124)
Tissue lysates, normalized for total protein (20
μg/lane), were from a 4 week old male mouse. BAG3
expression was detected at highest levels in skeletal
(quadriceps and diaphragm) and smooth (heart) muscle
specimens.All lanes: Western blot - Anti-Bag3 antibody (ab47124) at 1/2000 dilution
Lane 1: Thymus lysates, total protein (20ug/lane)
Lane 2: Spleen lysates, total protein (20ug/lane)
Lane 3: Skeletal(quadriceps) lysates, total protein (20ug/lane)
Lane 4: Skeletal(diaphragm) lysates, total protein (20ug/lane)
Lane 5: Smooth (heart) muscle lysates, total protein (20ug/lane)
Predicted band size: 62 kDa
Observed band size: 86 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (ab47124)
ICC/IF image of ab47124 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47124) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunohistochemistry (Frozen sections) - Anti-Bag3 antibody (ab47124)
Frozen mouse muscle tissue section stained for BAG3
expression using ab47124 at 1/2000. The tissue
section was fixed in 3.8% paraformaldehyde prior to
staining. BAG3 localizes with Z-disk proteins."
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bag3 antibody (ab47124)
Immunohistochemistry of formalin fixed
paraffin embedded human pancreas tissue sections
using ab47124 at 1/2000. A, normal pancreas. B and
C, pancreatic cancer. Hematoxylin-eosin counterstain.
Western blot - Anti-Bag3 antibody (ab47124)
All lanes: Western blot - Anti-Bag3 antibody (ab47124)
All lanes: Hela lysate at 0.5 mg/mL
Predicted band size: 62 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Bag3 antibody (ab47124)
Immunocytochemistry/Immunofluorescence of A431 cells stained with Anti-Bag3 antibody (ab47124) green. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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