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AB92309

Anti-Bag3 antibody [EPR3515]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

5

(1 Review)

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(20 Publications)

Rabbit Recombinant Monoclonal BAG3 antibody. Suitable for IP, WB and reacts with Mouse, Human samples. Cited in 20 publications.

View Alternative Names

BIS, BAG3, BAG family molecular chaperone regulator 3, BAG-3, Bcl-2-associated athanogene 3, Bcl-2-binding protein Bis, Docking protein CAIR-1

3 Images
Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (AB92309)
  • IP

Lab

Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (AB92309)

Purified ab92309 at 1/50 dilution (2μg) immunoprecipitating Bag3 in Mouse heart lysate.
Lane 1 (input) : Mouse heart lysate 10μg
Lane 2 (+) : ab92309 + Mouse heart lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab92309 in Mouse heart lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 80 kDa

All lanes:

Immunoprecipitation - Anti-Bag3 antibody [EPR3515] (ab92309)

Predicted band size: 62 kDa

false

Western blot - Anti-Bag3 antibody [EPR3515] (AB92309)
  • WB

Unknown

Western blot - Anti-Bag3 antibody [EPR3515] (AB92309)

All lanes:

Western blot - Anti-Bag3 antibody [EPR3515] (ab92309) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 62 kDa

Observed band size: 80 kDa

false

Western blot - Anti-Bag3 antibody [EPR3515] (AB92309)
  • WB

Lab

Western blot - Anti-Bag3 antibody [EPR3515] (AB92309)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : BAG3 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : K562 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab92309 observed at 80 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab92309 was shown to recognize BAG3 in wild-type cells as signal was lost at the expected MW in BAG3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and BAG3 knockout samples were subjected to SDS-PAGE. ab92309 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Bag3 antibody [EPR3515] (ab92309)

Predicted band size: 62 kDa

false

  • Carrier free

    Anti-Bag3 antibody [EPR3515] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3515

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcl-2-associated athanogene 3 commonly known as Bag3 is a protein with a molecular weight of approximately 74 kDa. It plays an important role in apoptosis regulation and stress responses by interacting with heat shock proteins. Bag3 is expressed in various tissues including the heart skeletal muscles and certain types of neurons. It functions mechanically by binding to chaperones like Hsp70 modulating their activity and helping in protein folding and degradation processes.
Biological function summary

Bag3 contributes to cell survival by assisting in protein homeostasis and mitigating stress-related cellular damage. It acts as a co-chaperone and forms part of complexes with Hsp70 HspB8 and CHIP among others. These interactions enhance the cellular capacity to handle misfolded proteins preventing the aggregation that could lead to cell toxicity. In muscle cells Bag3 plays a role in maintaining structural integrity by participating in the assembly of myofibrils critical for muscle function.

Pathways

The protein is involved in autophagy and apoptosis regulation. It actively participates in the ubiquitin-proteasome system and chaperone-mediated autophagy collaborating closely with proteins like HspB8 and Hsp70. The integration of Bag3 within these pathways highlights its involvement in removing damaged proteins and preserving cellular function during stress conditions. Additionally Bag3 contributes to the regulation of the NF-kB pathway which is involved in the immune response and inflammation.

Mutations or dysregulation of Bag3 associate with conditions such as myopathy and certain cancers. In myopathy aberrations in Bag3 affect muscle resilience and repair leading to structural muscle defects. In cancer Bag3's role in inhibiting apoptosis promotes tumor cell survival making it a target for therapeutic interventions. The protein has connections with p53 in cancer contexts where it influences cell death and survival decisions. Understanding Bag3's interactions and functions in these diseases opens new opportunities for developing treatments targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Co-chaperone and adapter protein that connects different classes of molecular chaperones including heat shock proteins 70 (HSP70s), e.g. HSPA1A/HSP70 or HSPA8/HSC70, and small heat shock proteins (sHSPs), e.g. HSPB8 (PubMed : 27884606, PubMed : 30559338). Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from HSP70s, thereby triggering client protein release (PubMed : 27884606, PubMed : 30559338). Nucleotide release is mediated via BAG3 binding to the nucleotide-binding domain (NBD) of HSP70s, whereas client release is mediated via binding to the substrate-binding domain (SBD) (PubMed : 27474739, PubMed : 9873016). Has anti-apoptotic activity (PubMed : 10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed : 26159920).
See full target information BAG3
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cell discovery 11:19 PubMed40038243

2025

RNA cytidine acetyltransferase NAT10 maintains T cell pathogenicity in inflammatory bowel disease.

Applications

Unspecified application

Species

Unspecified reactive species

Haixin Li,Xuemin Cai,Changfen Xu,Xinhui Yang,Xiaohan Song,Yuxin Kong,Mei Yang,Qielan Wu,Song Guo Zheng,Yiming Shao,Ping Wang,Jing Zhou,Hua-Bing Li

iScience 27:111423 PubMed39687015

2024

Integrated proteomic and transcriptomic landscape of human placenta in small for gestational age infants.

Applications

Unspecified application

Species

Unspecified reactive species

Heyue Jin,Xianyan Wang,Lingyu Li,Chen Rui,Hong Gan,Qunan Wang,Fangbiao Tao,Yumin Zhu

Oncotarget 15:124-133 PubMed38329728

2024

GZ17-6.02 interacts with bexarotene to kill mycosis fungoides cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael R Booth,Laurence Booth,Jane L Roberts,Cameron West,Paul Dent

Epigenetics 18:2239607 PubMed37523636

2023

Oncogenic DNA methyltransferase 1 activates the PI3K/AKT/mTOR signalling by blocking the binding of HSPB8 and BAG3 in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yemei Yang,Shengfang Ma,Zi Ye,Yushi Zheng,Zhenjiong Zheng,Xiongshan Liu,Xianyi Zhou

Cell cycle (Georgetown, Tex.) 22:1544-1562 PubMed37287276

2023

Exosomal miR-18a-5p promotes EMT and metastasis of NPC cells via targeting BTG3 and activating the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Zhong,Qihong Nie,Renrui Wu,Yun Huang

Protein science : a publication of the Protein Society 32:e4687 PubMed37243950

2023

HspB8 interacts with BAG3 in a "native-like" conformation forming a complex that displays chaperone-like activity.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Sciandrone,Diletta Ami,Annalisa D'Urzo,Elena Angeli,Annalisa Relini,Marco Vanoni,Antonino Natalello,Maria Elena Regonesi

Cell death discovery 9:159 PubMed37173310

2023

N-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyang Qi,Chi Zhang,Huan Jian,Mengfan Hou,Yongfu Lou,Yi Kang,Wei Wang,Yigang Lv,Shenghui Shang,Chaoyu Wang,Xueying Li,Shiqing Feng,Hengxing Zhou

Applied biological chemistry 66:10 PubMed36815904

2023

Baicalein facilitates gastric cancer cell apoptosis by triggering endoplasmic reticulum stress via repression of the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Shen,Zhiwen Yang,Xinlin Wu,Guodong Yao,Mingxing Hou

Frontiers in oncology 12:1045459 PubMed36408163

2022

GZ17-6.02 kills prostate cancer cells and .

Applications

Unspecified application

Species

Unspecified reactive species

Laurence Booth,Jane L Roberts,Cameron West,Paul Dent

Aging 14:8221-8242 PubMed36227739

2022

AR12 increases BAG3 expression which is essential for Tau and APP degradation via LC3-associated phagocytosis and macroautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Paul Dent,Laurence Booth,Jane L Roberts,Andrew Poklepovic,Jennifer Martinez,Derek Cridebring,Eric M Reiman
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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