AB97660

Anti-Bag5 antibody

Name: Anti-Bag5 antibody (ab97660) | Abcam
Brand: Abcam Limited
SKU: AB97660

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(5 Publications )

Rabbit Polyclonal BAG5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAG5 aa 1-200.

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Bag5 antibody (AB97660)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bag5 antibody (AB97660)

ab97660 at 1/200 dilution staining Bag5 in A549 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human BAG5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9UL15

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The BAG5 protein also known as BCL2-associated athanogene 5 plays an important role as a co-chaperone in the regulation of protein folding and degradation. It has a molecular weight of approximately 48 kDa. BAG5 is expressed in various tissues but shows higher levels in neuronal tissues. The protein interacts with components of the cellular machinery to influence the fate of client proteins affecting their stability and function. Its structural regions facilitate binding to both target proteins and other chaperones modulating chaperone activity.

Biological function summary

BAG5 influences processes related to cell survival and apoptosis regulation. It is involved in forming complexes with heat shock proteins particularly Hsp70 and Hsp90 affecting their chaperone activity. This interaction impacts the protein's role in maintaining cellular proteostasis under stress conditions. BAG5 also plays a role in modulating the balance between pro-survival and pro-apoptotic signals by regulating Bcl-2 family members integrating signals that affect cell fate decisions.

Pathways

BAG5 participates in cellular stress response and apoptosis pathways. It functions within the endoplasmic reticulum-associated degradation (ERAD) pathway where it helps in the recognition and disposal of misfolded proteins. BAG5 has related interactions with other proteins like CHIP (C-terminus of Hsc70-interacting protein) and ubiquitin-protein ligases which are important in the ubiquitin-proteasome system. These interactions position BAG5 as an important player in managing protein quality control alongside regulating stress responses.

BAG5 relates to neurodegenerative diseases such as Parkinson's disease (PD) and Alzheimer's disease (AD). The protein interacts with Parkinson’s disease-associated protein alpha-synuclein influencing its aggregation and toxicity. In Alzheimer's disease BAG5 modulates the function of tau protein which aggregates abnormally in affected neurons. Regulation of these proteins by BAG5 impacts disease progression highlighting BAG5 as a potential therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Co-chaperone for HSP/HSP70 proteins. It functions as a nucleotide-exchange factor promoting the release of ADP from HSP70, thereby activating HSP70-mediated protein refolding (PubMed : 20223214). Has an essential role in maintaining proteostasis at junctional membrane complexes (JMC), where it may function as a scaffold between the HSPA8 chaperone and JMC proteins enabling correct, HSPA8-dependent JMC protein folding (By similarity). Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity).

See full target information BAG5

Alternative Names

KIAA0873, BAG5, BAG family molecular chaperone regulator 5, BAG-5, Bcl-2-associated athanogene 5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:9230435 PubMed34777698

2021

miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A.

Applications

Unspecified application

Species

Unspecified reactive species

Haoming Lin,Rui Zhang,Wenrui Wu,Liming Lei

Experimental and therapeutic medicine 22:664 PubMed33986829

2021

miRNA-770-5p expression is upregulated in patients with type 2 diabetes and miRNA-770-5p knockdown protects pancreatic β-cell function via targeting BAG5 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Jilou Wei,Ting Ji,Kui Zang

Environmental toxicology 36:620-627 PubMed33283947

2020

MiRNA-429 alleviates ketamine-induced neurotoxicity through targeting BAG5.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Fan,Wenchao Bian,Meichen Liu,Jinjie Li,Yunyun Wang

Molecular medicine reports 21:1011-1020 PubMed31922242

2020

miR‑155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Xi,Qiang-Qiang Li,Bing-Qiang Li,Ning Li

BMC cancer 13:96 PubMed23448667

2013

Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis.

Applications

ICC/IF, WB

Species

Human, Human

Anja Bruchmann,Corinna Roller,Tamara Vanessa Walther,Georg Schäfer,Sara Lehmusvaara,Tapio Visakorpi,Helmut Klocker,Andrew C B Cato,Danilo Maddalo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bag5 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Bag5 antibody (AB97660)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (5)

Product promise