Rabbit Polyclonal BAG5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAG5 aa 1-200.
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Publications
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- Oxidative medicine and cellular longevity 2021:92304352021miR-4454 Promotes Hepatic Carcinoma Progression by Targeting Vps4A and Rab27A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaoming Lin,Rui Zhang,Wenrui Wu,Liming LeiPubMed 34777698
- Experimental and therapeutic medicine 22:6642021miRNA-770-5p expression is upregulated in patients with type 2 diabetes and miRNA-770-5p knockdown protects pancreatic β-cell function via targeting BAG5 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Wang,Jilou Wei,Ting Ji,Kui ZangPubMed 33986829
- Environmental toxicology 36:620-6272020MiRNA-429 alleviates ketamine-induced neurotoxicity through targeting BAG5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaodi Fan,Wenchao Bian,Meichen Liu,Jinjie Li,Yunyun WangPubMed 33283947
- Molecular medicine reports 21:1011-10202020miR‑155 inhibition represents a potential valuable regulator in mitigating myocardial hypoxia/reoxygenation injury through targeting BAG5 and MAPK/JNK signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Xi et. al.PubMed 31922242
- BMC cancer 13:962013Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis.Applications:ICC/IF, WBReactive species:HumanAnja Bruchmann,Corinna Roller,Tamara Vanessa Walther,Georg Schäfer,Sara Lehmusvaara,Tapio Visakorpi,Helmut Klocker,Andrew C B Cato,Danilo MaddaloPubMed 23448667
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human BAG5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UL15
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Co-chaperone for HSP/HSP70 proteins. It functions as a nucleotide-exchange factor promoting the release of ADP from HSP70, thereby activating HSP70-mediated protein refolding (PubMed:20223214). Has an essential role in maintaining proteostasis at junctional membrane complexes (JMC), where it may function as a scaffold between the HSPA8 chaperone and JMC proteins enabling correct, HSPA8-dependent JMC protein folding (By similarity). Inhibits both auto-ubiquitination of PRKN and ubiquitination of target proteins by PRKN (By similarity).
Alternative names
KIAA0873, BAG5, BAG family molecular chaperone regulator 5, BAG-5, Bcl-2-associated athanogene 5
Recommended products
Rabbit Polyclonal BAG5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAG5 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human BAG5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UL15
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The BAG5 protein also known as BCL2-associated athanogene 5 plays an important role as a co-chaperone in the regulation of protein folding and degradation. It has a molecular weight of approximately 48 kDa. BAG5 is expressed in various tissues but shows higher levels in neuronal tissues. The protein interacts with components of the cellular machinery to influence the fate of client proteins affecting their stability and function. Its structural regions facilitate binding to both target proteins and other chaperones modulating chaperone activity.
- Biological function summary
BAG5 influences processes related to cell survival and apoptosis regulation. It is involved in forming complexes with heat shock proteins particularly Hsp70 and Hsp90 affecting their chaperone activity. This interaction impacts the protein's role in maintaining cellular proteostasis under stress conditions. BAG5 also plays a role in modulating the balance between pro-survival and pro-apoptotic signals by regulating Bcl-2 family members integrating signals that affect cell fate decisions.
- Pathways
BAG5 participates in cellular stress response and apoptosis pathways. It functions within the endoplasmic reticulum-associated degradation (ERAD) pathway where it helps in the recognition and disposal of misfolded proteins. BAG5 has related interactions with other proteins like CHIP (C-terminus of Hsc70-interacting protein) and ubiquitin-protein ligases which are important in the ubiquitin-proteasome system. These interactions position BAG5 as an important player in managing protein quality control alongside regulating stress responses.
- Associated diseases and disorders
BAG5 relates to neurodegenerative diseases such as Parkinson's disease (PD) and Alzheimer's disease (AD). The protein interacts with Parkinson’s disease-associated protein alpha-synuclein influencing its aggregation and toxicity. In Alzheimer's disease BAG5 modulates the function of tau protein which aggregates abnormally in affected neurons. Regulation of these proteins by BAG5 impacts disease progression highlighting BAG5 as a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Bag5 antibody (ab97660)
10% SDS-PAGE.
All lanes: Western blot - Anti-Bag5 antibody (ab97660) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: MOLT4 whole cell lysate at 30 µg
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Bag5 antibody (ab97660)
ab97660 at 1/200 dilution staining Bag5 in A549 cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
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