AB69404

Anti-Bak antibody

(1 Review)

(6 Publications)

Rabbit Polyclonal BAK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BAK1 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BAK, BCL2L7, CDN1, BAK1, Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (AB69404)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (AB69404)

Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.

Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (AB69404)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (AB69404)

Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5.

Unknown

Western blot - Anti-Bak antibody (AB69404)

All lanes:

Western blot - Anti-Bak antibody (ab69404) at 1/1000 dilution

All lanes:

A431 cell lysate

Predicted band size: 23 kDa

Observed band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human BAK1 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q16611

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bak protein also known as Bcl-2 homologous antagonist killer plays a mechanical role in the mitochondrial apoptosis pathway. It often functions as a pro-apoptotic member of the Bcl-2 protein family. Bak is expressed widely in the body being present in tissues such as the brain and heart. The molecular weight of Bak is approximately 23 kilodaltons. Bak interacts with other mitochondrial proteins to trigger cell death processes.

Biological function summary

Bak engages in promoting apoptosis by disrupting the mitochondrial outer membrane potential. It is part of the Bcl-2 family complex which balances cell survival and cell death. Bak collaborates with Bax protein to form pores in the mitochondrial membrane releasing cytochrome c and other apoptotic factors. This process initiates the cascade of caspases leading to programmed cell death.

Pathways

Bak is a critical component of the intrinsic apoptotic pathway. It directly interacts with pro-apoptotic proteins like Bax and anti-apoptotic proteins such as Bcl-2 and Bcl-xL. Bak's action in the apoptotic pathway involves a delicate balance between survival and death signals affecting cellular homeostasis. In addition Bak's interaction with the p53 pathway emphasizes its role in response to DNA damage ensuring damaged cells do not proliferate uncontrollably.

Malfunction or dysregulation of Bak can lead to cancer and neurodegenerative disorders. In cancer reduced Bak activity may allow cells to evade apoptosis promoting tumor survival and growth. Conversely in diseases like Parkinson's excessive Bak activity may result in enhanced neuronal apoptosis. Bak's interaction with proteins such as Bcl-2 also makes it a potential target for therapies aimed at restoring apoptotic balance in affected cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.

See full target information BAK1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Blood cancer journal 11:111 PubMed34099621

2021

The combination of CUDC-907 and gilteritinib shows promising in vitro and in vivo antileukemic activity against FLT3-ITD AML.

Applications

Unspecified application

Species

Unspecified reactive species

Xinan Qiao,Jun Ma,Tristan Knight,Yongwei Su,Holly Edwards,Lisa Polin,Jing Li,Juiwanna Kushner,Sijana H Dzinic,Kathryn White,Jian Wang,Hai Lin,Yue Wang,Liping Wang,Guan Wang,Jeffrey W Taub,Yubin Ge

Frontiers in veterinary science 8:570982 PubMed33748203

2021

Canine Papillomavirus 2 E6 Does Not Interfere With UVB-Induced Upregulation of p53 and p53-Regulated Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Quinlan,Susan May,Ryan Weeks,Hang Yuan,Jennifer Luff

BMC cancer 20:665 PubMed32677906

2020

Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qingyuan Feng,Xuzhao Bian,Xuan Liu,Ying Wang,Huiting Zhou,Xiaojing Ma,Chunju Quan,Yi Yao,Zhongliang Zheng

Experimental and therapeutic medicine 15:235-241 PubMed29375685

2018

BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Yuan,Luqiao Pu,Zhiliang He,Jian Wang

Oncology reports 38:2705-2716 PubMed29048629

2017

Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiheng Li,Xiaolu Li,Lixiao Xu,Yanfang Tao,Chun Yang,Xiaolan Chen,Fang Fang,Yi Wu,Xin Ding,He Zhao,Mei Li,Guanghui Qian,Yunyun Xu,Junli Ren,Weiwei Du,Jian Wang,Jun Lu,Shaoyan Hu,Jian Pan

Oncogene 36:6863-6872 PubMed28869605

2017

Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

G Chen,B Zhang,H Xu,Y Sun,Y Shi,Y Luo,H Jia,F Wang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Bak antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (AB69404)

Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (AB69404)

Western blot - Anti-Bak antibody (AB69404)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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