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Mouse Monoclonal BAK antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAK1 aa 1-200.


Images

Flow Cytometry - Anti-Bak antibody [AT38E2] (AB104124), expandable thumbnail
  • Western blot - Anti-Bak antibody [AT38E2] (AB104124), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (AB104124), expandable thumbnail
  • Western blot - Anti-Bak antibody [AT38E2] (AB104124), expandable thumbnail
  • Western blot - Anti-Bak antibody [AT38E2] (AB104124), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human BAK1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q16611

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.

Alternative names

BAK, BCL2L7, CDN1, BAK1, Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7

Recommended products

Mouse Monoclonal BAK antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BAK1 aa 1-200.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human BAK1 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q16611
Clone number
AT38E2
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Bak protein also known as Bcl-2 homologous antagonist killer plays a mechanical role in the mitochondrial apoptosis pathway. It often functions as a pro-apoptotic member of the Bcl-2 protein family. Bak is expressed widely in the body being present in tissues such as the brain and heart. The molecular weight of Bak is approximately 23 kilodaltons. Bak interacts with other mitochondrial proteins to trigger cell death processes.

Biological function summary

Bak engages in promoting apoptosis by disrupting the mitochondrial outer membrane potential. It is part of the Bcl-2 family complex which balances cell survival and cell death. Bak collaborates with Bax protein to form pores in the mitochondrial membrane releasing cytochrome c and other apoptotic factors. This process initiates the cascade of caspases leading to programmed cell death.

Pathways

Bak is a critical component of the intrinsic apoptotic pathway. It directly interacts with pro-apoptotic proteins like Bax and anti-apoptotic proteins such as Bcl-2 and Bcl-xL. Bak's action in the apoptotic pathway involves a delicate balance between survival and death signals affecting cellular homeostasis. In addition Bak's interaction with the p53 pathway emphasizes its role in response to DNA damage ensuring damaged cells do not proliferate uncontrollably.

Associated diseases and disorders

Malfunction or dysregulation of Bak can lead to cancer and neurodegenerative disorders. In cancer reduced Bak activity may allow cells to evade apoptosis promoting tumor survival and growth. Conversely in diseases like Parkinson's excessive Bak activity may result in enhanced neuronal apoptosis. Bak's interaction with proteins such as Bcl-2 also makes it a potential target for therapies aimed at restoring apoptotic balance in affected cells.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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6 product images

  • Flow Cytometry - Anti-Bak antibody [AT38E2] (ab104124), expandable thumbnail

    Flow Cytometry - Anti-Bak antibody [AT38E2] (ab104124)

    Flow cytometry analysis of BAK in HeLa cells. The cell was stained with ab104124 at 2-5μg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • Western blot - Anti-Bak antibody [AT38E2] (ab104124), expandable thumbnail

    Western blot - Anti-Bak antibody [AT38E2] (ab104124)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: BAK knockout HAP1 whole cell lysate (20 μg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab104124 observed at 0 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab104124 was found to be non-specific when BAK knockout samples were used. Wild-type and BAK knockout samples were subjected to SDS-PAGE. ab104124 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/250 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This image was produced using the ascites version of this antibody.

    All lanes: Western blot - Anti-Bak antibody [AT38E2] (ab104124)

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)

    Immunocytochemistry/ Immunofluorescence analysis of BAK in HeLa cells. The cell was stained with ab104124 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

  • Western blot - Anti-Bak antibody [AT38E2] (ab104124), expandable thumbnail

    Western blot - Anti-Bak antibody [AT38E2] (ab104124)

    All lanes: Western blot - Anti-Bak antibody [AT38E2] (ab104124) at 1/1000 dilution

    Lane 1: 293T cell lysate at 40 µg

    Lane 2: HeLa cell lysate at 40 µg

    Lane 3: A431 cell lysate at 40 µg

    Lane 4: A549 cell lysate at 40 µg

    Lane 5: Jurkat cell lysate at 40 µg

    Lane 6: MCF7 cell lysate at 40 µg

    Lane 7: PC3 cell lysate at 40 µg

    Secondary

    All lanes: goat anti-mouse secondary antibody conjugated to HRP

    Predicted band size: 23 kDa

  • Western blot - Anti-Bak antibody [AT38E2] (ab104124), expandable thumbnail

    Western blot - Anti-Bak antibody [AT38E2] (ab104124)

    This image was produced using the ascites version of this antibody.

    All lanes: Western blot - Anti-Bak antibody [AT38E2] (ab104124) at 1/500 dilution

    All lanes: Mouse lung lyasate at 35 µg

    Secondary

    All lanes: Goat anti-mouse HRP conjugate

    Developed using the ECL technique.

    Predicted band size: 23 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody [AT38E2] (ab104124)

    Immunofluorescence staining of Bak in HeLa cells using Hoechst 3342 (Blue) for nucleus staining and ab104124 antibody (1/500) with Texas Red (Red).

    This image was produced using the ascites version of this antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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