AB78067

Anti-Band 3/AE 1 antibody

Name: Anti-Band 3/AE 1 antibody (ab78067) | Abcam
Brand: Abcam Limited
SKU: AB78067
Price: 630 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Band 3/AE 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD233, AE1, DI, EPB3, SLC4A1, Band 3 anion transport protein, Anion exchange protein 1, Solute carrier family 4 member 1, AE 1, Anion exchanger 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody (AB78067)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody (AB78067)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Band 3/AE 1 with ab78067 at 10 μg/ml.

Unknown

Western blot - Anti-Band 3/AE 1 antibody (AB78067)

All lanes:

Western blot - Anti-Band 3/AE 1 antibody (ab78067) at 1 µg/mL

Lane 1:

Liver (Human) Tissue Lysate - fetal normal tissue (<a href='/en-us/products/unavailable/liver-human-tissue-lysate-fetal-normal-tissue-ab29890'>ab29890</a>) at 10 µg

Lane 2:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 101 kDa

Observed band size: 60 kDa,90 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Band 3 also known as AE1 or Anion Exchanger 1 is an important protein found in red blood cells with a mass of approximately 100 kDa. It primarily facilitates the exchange of chloride (Cl-) and bicarbonate (HCO3-) ions across the cell membrane. This protein plays a significant role in maintaining the acid-base balance in the blood and in carbon dioxide transport. Besides red blood cells Band 3 is also expressed in the kidney specifically in the basolateral membrane of alpha-intercalated cells.

Biological function summary

Band 3 acts as a component of the larger protein complex responsible for the anion exchange process. It interacts with other proteins such as ankyrin and spectrin forming a structural link between the plasma membrane and the cytoskeleton. This interaction is essential in maintaining the red blood cell shape and flexibility. Band 3 also serves as a docking site for various glycolytic enzymes contributing to metabolic regulation within the cell.

Pathways

Band 3 plays a part in the bicarbonate transport pathway critical for regulating blood pH and carbon dioxide removal from tissues to the lungs. It closely interacts with carbonic anhydrase a protein that catalyzes the conversion between carbon dioxide and bicarbonate. Another pathway where Band 3 is involved is the erythrocyte cytoskeleton organization pathway in which it works with proteins like spectrin to stabilize the red blood cell membrane.

Band 3 mutations or deficiencies can lead to conditions like hereditary spherocytosis and distal renal tubular acidosis. Hereditary spherocytosis marked by hemolytic anemia involves the improper formation of red blood cells due to defective Band 3 interactions with ankyrin. Meanwhile a malfunction in Band 3's ion exchange capacity can result in distal renal tubular acidosis affecting the kidneys' ability to maintain acid-base homeostasis. Scientists study these conditions to understand better how Band 3 functions across different cell types and its interactions with related proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed : 10926824, PubMed : 14734552, PubMed : 1538405, PubMed : 16227998, PubMed : 20151848, PubMed : 24121512, PubMed : 28387307, PubMed : 35835865). Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin (PubMed : 1538405, PubMed : 20151848, PubMed : 35835865). Functions as a transporter that mediates the 1 : 1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (PubMed : 10926824, PubMed : 14734552, PubMed : 16227998, PubMed : 24121512, PubMed : 28387307).. (Microbial infection) Acts as a receptor for P.falciparum (isolate 3D7) MSP9 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 14630931). Acts as a receptor for P.falciparum (isolate 3D7) MSP1 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 12692305).

See full target information SLC4A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Structure (London, England : 1993) 25:132-145 PubMed27989623

2016

Full-Length Anion Exchanger 1 Structure and Interactions with Ankyrin-1 Determined by Zero Length Crosslinking of Erythrocyte Membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Roland Rivera-Santiago,Sandra L Harper,Sira Sriswasdi,Peter Hembach,David W Speicher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Band 3/AE 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody (AB78067)

Western blot - Anti-Band 3/AE 1 antibody (AB78067)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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