JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249866

Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Band 3/AE 1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CD233, AE1, DI, EPB3, SLC4A1, Band 3 anion transport protein, Anion exchange protein 1, Solute carrier family 4 member 1, AE 1, Anion exchanger 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (AB249866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (AB249866)

This data was developed using ab175214, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Band 3/AE 1 wtih ab175214 at 1/250 dilution. Note erythrocyte staining. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (AB249866)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (AB249866)

This data was developed using ab175214, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Band 3/AE 1 at 1/250 dilution. Note erythrocyte staining. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (AB249866)
  • WB

Supplier Data

Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] - BSA and Azide free (AB249866)

This data was developed using ab175214, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] (<a href='/en-us/products/primary-antibodies/band-3-ae-1-antibody-epr14252-ab175214'>ab175214</a>) at 1/100000 dilution

All lanes:

Human red blood cell lysate at 10 µg

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1425(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB129024

Anti-Glycophorin A antibody [EPR8200]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycophorin A antibody [EPR8200] (AB129024)

  • 5
  • 1
Primary Antibodies

AB92492

Anti-Hemoglobin subunit alpha antibody [EPR3608]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hemoglobin subunit alpha antibody [EPR3608] (AB92492)

  • 4
  • 2
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab249866 is the carrier-free version of ab175214.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Band 3 also known as AE1 or Anion Exchanger 1 is an important protein found in red blood cells with a mass of approximately 100 kDa. It primarily facilitates the exchange of chloride (Cl-) and bicarbonate (HCO3-) ions across the cell membrane. This protein plays a significant role in maintaining the acid-base balance in the blood and in carbon dioxide transport. Besides red blood cells Band 3 is also expressed in the kidney specifically in the basolateral membrane of alpha-intercalated cells.
Biological function summary

Band 3 acts as a component of the larger protein complex responsible for the anion exchange process. It interacts with other proteins such as ankyrin and spectrin forming a structural link between the plasma membrane and the cytoskeleton. This interaction is essential in maintaining the red blood cell shape and flexibility. Band 3 also serves as a docking site for various glycolytic enzymes contributing to metabolic regulation within the cell.

Pathways

Band 3 plays a part in the bicarbonate transport pathway critical for regulating blood pH and carbon dioxide removal from tissues to the lungs. It closely interacts with carbonic anhydrase a protein that catalyzes the conversion between carbon dioxide and bicarbonate. Another pathway where Band 3 is involved is the erythrocyte cytoskeleton organization pathway in which it works with proteins like spectrin to stabilize the red blood cell membrane.

Band 3 mutations or deficiencies can lead to conditions like hereditary spherocytosis and distal renal tubular acidosis. Hereditary spherocytosis marked by hemolytic anemia involves the improper formation of red blood cells due to defective Band 3 interactions with ankyrin. Meanwhile a malfunction in Band 3's ion exchange capacity can result in distal renal tubular acidosis affecting the kidneys' ability to maintain acid-base homeostasis. Scientists study these conditions to understand better how Band 3 functions across different cell types and its interactions with related proteins.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed : 10926824, PubMed : 14734552, PubMed : 1538405, PubMed : 16227998, PubMed : 20151848, PubMed : 24121512, PubMed : 28387307, PubMed : 35835865). Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin (PubMed : 1538405, PubMed : 20151848, PubMed : 35835865). Functions as a transporter that mediates the 1 : 1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (PubMed : 10926824, PubMed : 14734552, PubMed : 16227998, PubMed : 24121512, PubMed : 28387307).. (Microbial infection) Acts as a receptor for P.falciparum (isolate 3D7) MSP9 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 14630931). Acts as a receptor for P.falciparum (isolate 3D7) MSP1 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 12692305).
See full target information SLC4A1
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com