AB108414

Anti-Band 3/AE 1 antibody [EPR1426]

Name: Anti-Band 3/AE 1 antibody [EPR1426] (ab108414) | Abcam
Brand: Abcam Limited
SKU: AB108414
Price: 390 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(13 Publications)

Rabbit Recombinant Monoclonal Band 3/AE 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 13 publications.

View Alternative Names

CD233, AE1, DI, EPB3, SLC4A1, Band 3 anion transport protein, Anion exchange protein 1, Solute carrier family 4 member 1, AE 1, Anion exchanger 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human lung tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

All lanes:

Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414) at 1/1000 dilution

All lanes:

Red blood cell lysate at 10 µg

Predicted band size: 101 kDa

false

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Band 3/AE 1 antibody [EPR1426]
Carrier free

Anti-Band 3/AE 1 antibody [EPR1426] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Band 3/AE 1 antibody [EPR1426]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1426

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Band 3 also known as AE1 or Anion Exchanger 1 is an important protein found in red blood cells with a mass of approximately 100 kDa. It primarily facilitates the exchange of chloride (Cl-) and bicarbonate (HCO3-) ions across the cell membrane. This protein plays a significant role in maintaining the acid-base balance in the blood and in carbon dioxide transport. Besides red blood cells Band 3 is also expressed in the kidney specifically in the basolateral membrane of alpha-intercalated cells.

Biological function summary

Band 3 acts as a component of the larger protein complex responsible for the anion exchange process. It interacts with other proteins such as ankyrin and spectrin forming a structural link between the plasma membrane and the cytoskeleton. This interaction is essential in maintaining the red blood cell shape and flexibility. Band 3 also serves as a docking site for various glycolytic enzymes contributing to metabolic regulation within the cell.

Pathways

Band 3 plays a part in the bicarbonate transport pathway critical for regulating blood pH and carbon dioxide removal from tissues to the lungs. It closely interacts with carbonic anhydrase a protein that catalyzes the conversion between carbon dioxide and bicarbonate. Another pathway where Band 3 is involved is the erythrocyte cytoskeleton organization pathway in which it works with proteins like spectrin to stabilize the red blood cell membrane.

Band 3 mutations or deficiencies can lead to conditions like hereditary spherocytosis and distal renal tubular acidosis. Hereditary spherocytosis marked by hemolytic anemia involves the improper formation of red blood cells due to defective Band 3 interactions with ankyrin. Meanwhile a malfunction in Band 3's ion exchange capacity can result in distal renal tubular acidosis affecting the kidneys' ability to maintain acid-base homeostasis. Scientists study these conditions to understand better how Band 3 functions across different cell types and its interactions with related proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed : 10926824, PubMed : 14734552, PubMed : 1538405, PubMed : 16227998, PubMed : 20151848, PubMed : 24121512, PubMed : 28387307, PubMed : 35835865). Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin (PubMed : 1538405, PubMed : 20151848, PubMed : 35835865). Functions as a transporter that mediates the 1 : 1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (PubMed : 10926824, PubMed : 14734552, PubMed : 16227998, PubMed : 24121512, PubMed : 28387307).. (Microbial infection) Acts as a receptor for P.falciparum (isolate 3D7) MSP9 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 14630931). Acts as a receptor for P.falciparum (isolate 3D7) MSP1 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 12692305).

See full target information SLC4A1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15789 PubMed38982195

2024

Human red blood cells express the RNA sensor TLR7.

Applications

Unspecified application

Species

Unspecified reactive species

L K Metthew Lam,Emily Oatman,Kaitlyn A Eckart,Nathan J Klingensmith,Emily Flowers,Layal Sayegh,Julia Yuen,Rebecca L Clements,Nuala J Meyer,Kellie A Jurado,Andrew E Vaughan,Stephanie C Eisenbarth,Nilam S Mangalmurti

Frontiers in physiology 15:1399154 PubMed38706947

2024

Mechanism of hypoxia-induced damage to the mechanical property in human erythrocytes-band 3 phosphorylation and sulfhydryl oxidation of membrane proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Qinqin Yang,Dong Chen,Chungong Li,Runjing Liu,Xiang Wang

Frontiers in physiology 14:1278763 PubMed37916221

2023

Changes in hemoglobin oxidation and band 3 during blood storage impact oxygen sensing and mitochondrial bioenergetic pathways in the human pulmonary arterial endothelial cell model.

Applications

Unspecified application

Species

Unspecified reactive species

Sirsendu Jana,Tigist Kassa,Francine Wood,Wayne Hicks,Abdu I Alayash

Proceedings of the National Academy of Sciences of the United States of America 119:e2210819119 PubMed36454755

2022

Fatigue of red blood cells under periodic squeezes in ECMO.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfan Pan,Yan Li,Yongjian Li,Jiang Li,Haosheng Chen

Journal of clinical medicine 11: PubMed35743501

2022

Comparison of the Hemocompatibility of an Axial and a Centrifugal Left Ventricular Assist Device in an In Vitro Test Circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Borchers,Patrick Winnersbach,Sandra Kraemer,Christian Beckers,Eva Miriam Buhl,Steffen Leonhardt,Rolf Rossaint,Marian Walter,Thomas Breuer,Christian Bleilevens

Experimental and therapeutic medicine 24:480 PubMed35761813

2022

Vitamin E-coated dialyzer alleviates erythrocyte deformability dysfunction in patients with end-stage renal disease undergoing hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhang,Wei Gao,Xia Li

Oxidative medicine and cellular longevity 2020:7834252 PubMed32963701

2020

Downregulated Recycling Process but Not De Novo Synthesis of Glutathione Limits Antioxidant Capacity of Erythrocytes in Hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Yueming Wang,Nannan Zhao,Yanlei Xiong,Jiashen Zhang,Dongmei Zhao,Yancun Yin,Lele Song,Yipeng Yin,Jing Wang,Xiying Luan,Yanlian Xiong

Journal of cellular and molecular medicine 23:4454-4463 PubMed31016877

2019

Two novel ANK1 loss-of-function mutations in Chinese families with hereditary spherocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Hao,Shanshan Li,Duan Ma,Shiyu Chen,Bowen Zhang,Deyong Xiao,Jin Zhang,Nan Jiang,Shayi Jiang,Jing Ma

Nature communications 9:4045 PubMed30279493

2018

Correcting glucose-6-phosphate dehydrogenase deficiency with a small-molecule activator.

Applications

Unspecified application

Species

Unspecified reactive species

Sunhee Hwang,Karen Mruk,Simin Rahighi,Andrew G Raub,Che-Hong Chen,Lisa E Dorn,Naoki Horikoshi,Soichi Wakatsuki,James K Chen,Daria Mochly-Rosen

Scientific reports 8:5052 PubMed29567995

2018

Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Tougan,Jyotheeswara R Edula,Eizo Takashima,Masayuki Morita,Miki Shinohara,Akira Shinohara,Takafumi Tsuboi,Toshihiro Horii

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Band 3/AE 1 antibody [EPR1426]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise