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Rabbit Monoclonal Band 3/AE 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.


Images

Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (AB108414), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed:10926824, PubMed:14734552, PubMed:1538405, PubMed:16227998, PubMed:20151848, PubMed:24121512, PubMed:28387307, PubMed:35835865). Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin (PubMed:1538405, PubMed:20151848, PubMed:35835865). Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (PubMed:10926824, PubMed:14734552, PubMed:16227998, PubMed:24121512, PubMed:28387307).(Microbial infection) Acts as a receptor for P.falciparum (isolate 3D7) MSP9 and thus, facilitates merozoite invasion of erythrocytes (PubMed:14630931). Acts as a receptor for P.falciparum (isolate 3D7) MSP1 and thus, facilitates merozoite invasion of erythrocytes (PubMed:12692305).

Alternative names

Recommended products

Rabbit Monoclonal Band 3/AE 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 10 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR1426

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Band 3 also known as AE1 or Anion Exchanger 1 is an important protein found in red blood cells with a mass of approximately 100 kDa. It primarily facilitates the exchange of chloride (Cl-) and bicarbonate (HCO3-) ions across the cell membrane. This protein plays a significant role in maintaining the acid-base balance in the blood and in carbon dioxide transport. Besides red blood cells Band 3 is also expressed in the kidney specifically in the basolateral membrane of alpha-intercalated cells.

Biological function summary

Band 3 acts as a component of the larger protein complex responsible for the anion exchange process. It interacts with other proteins such as ankyrin and spectrin forming a structural link between the plasma membrane and the cytoskeleton. This interaction is essential in maintaining the red blood cell shape and flexibility. Band 3 also serves as a docking site for various glycolytic enzymes contributing to metabolic regulation within the cell.

Pathways

Band 3 plays a part in the bicarbonate transport pathway critical for regulating blood pH and carbon dioxide removal from tissues to the lungs. It closely interacts with carbonic anhydrase a protein that catalyzes the conversion between carbon dioxide and bicarbonate. Another pathway where Band 3 is involved is the erythrocyte cytoskeleton organization pathway in which it works with proteins like spectrin to stabilize the red blood cell membrane.

Associated diseases and disorders

Band 3 mutations or deficiencies can lead to conditions like hereditary spherocytosis and distal renal tubular acidosis. Hereditary spherocytosis marked by hemolytic anemia involves the improper formation of red blood cells due to defective Band 3 interactions with ankyrin. Meanwhile a malfunction in Band 3's ion exchange capacity can result in distal renal tubular acidosis affecting the kidneys' ability to maintain acid-base homeostasis. Scientists study these conditions to understand better how Band 3 functions across different cell types and its interactions with related proteins.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414), expandable thumbnail

    Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

    All lanes: Western blot - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414) at 1/1000 dilution

    All lanes: Red blood cell lysate at 10 µg

    Predicted band size: 101 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

    ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)

    ab108414, at 1/100 dilution, staining Band 3/AE 1 in paraffin-embedded Human lung tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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