AB125070

Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Band 3/AE 1 phospho Y21 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD233, AE1, DI, EPB3, SLC4A1, Band 3 anion transport protein, Anion exchange protein 1, Solute carrier family 4 member 1, AE 1, Anion exchanger 1

2 Images

Lab

Western blot - Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (AB125070)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution

Lane 1:

Untreated RBC at 10 µg

Lane 2:

RBC treated with pervanadate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 101 kDa

Observed band size: 101 kDa

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Unknown

Western blot - Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (AB125070)

All lanes:

Western blot - Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (ab125070) at 1/50000 dilution

Lane 1:

Human red blood cell lysate, untreated at 10 µg

Lane 2:

Human red blood cell lysate, Pervanadate treated at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 101 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1424

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Band 3 also known as AE1 or Anion Exchanger 1 is an important protein found in red blood cells with a mass of approximately 100 kDa. It primarily facilitates the exchange of chloride (Cl-) and bicarbonate (HCO3-) ions across the cell membrane. This protein plays a significant role in maintaining the acid-base balance in the blood and in carbon dioxide transport. Besides red blood cells Band 3 is also expressed in the kidney specifically in the basolateral membrane of alpha-intercalated cells.

Biological function summary

Band 3 acts as a component of the larger protein complex responsible for the anion exchange process. It interacts with other proteins such as ankyrin and spectrin forming a structural link between the plasma membrane and the cytoskeleton. This interaction is essential in maintaining the red blood cell shape and flexibility. Band 3 also serves as a docking site for various glycolytic enzymes contributing to metabolic regulation within the cell.

Pathways

Band 3 plays a part in the bicarbonate transport pathway critical for regulating blood pH and carbon dioxide removal from tissues to the lungs. It closely interacts with carbonic anhydrase a protein that catalyzes the conversion between carbon dioxide and bicarbonate. Another pathway where Band 3 is involved is the erythrocyte cytoskeleton organization pathway in which it works with proteins like spectrin to stabilize the red blood cell membrane.

Band 3 mutations or deficiencies can lead to conditions like hereditary spherocytosis and distal renal tubular acidosis. Hereditary spherocytosis marked by hemolytic anemia involves the improper formation of red blood cells due to defective Band 3 interactions with ankyrin. Meanwhile a malfunction in Band 3's ion exchange capacity can result in distal renal tubular acidosis affecting the kidneys' ability to maintain acid-base homeostasis. Scientists study these conditions to understand better how Band 3 functions across different cell types and its interactions with related proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein (PubMed : 10926824, PubMed : 14734552, PubMed : 1538405, PubMed : 16227998, PubMed : 20151848, PubMed : 24121512, PubMed : 28387307, PubMed : 35835865). Component of the ankyrin-1 complex of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin (PubMed : 1538405, PubMed : 20151848, PubMed : 35835865). Functions as a transporter that mediates the 1 : 1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (PubMed : 10926824, PubMed : 14734552, PubMed : 16227998, PubMed : 24121512, PubMed : 28387307).. (Microbial infection) Acts as a receptor for P.falciparum (isolate 3D7) MSP9 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 14630931). Acts as a receptor for P.falciparum (isolate 3D7) MSP1 and thus, facilitates merozoite invasion of erythrocytes (PubMed : 12692305).

See full target information SLC4A1 phospho Y21

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Research (Washington, D.C.) 8:0809 PubMed40740652

2025

Combinational Analysis of Metabolomic and O-GlcNAcylation Omics Reveals the HBP Metabolic Regulation of Chemoresistance via GFPT1/NR3C1 O-GlcNAcylation/GPX4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhou,Chunlin Zhang,Li Li,Zhenwei Feng,Xuesong Bai,Yuhua Mei,Weiyang He,Xin Gou,Xinyuan Li

Scientific reports 14:31441 PubMed39733159

2024

Biopreservation and reversal of oxidative injury during blood storage by a novel curcumin-based gel formulation.

Applications

Unspecified application

Species

Unspecified reactive species

Wayne Hicks,Sirsendu Jana,Tigist Kassa,Richard Prince,Pedro Cabrales,Joel Friedman,Abdu I Alayash

Journal of translational medicine 21:676 PubMed37770909

2023

Tyrosine phosphorylation of band 3 impairs the storage quality of suspended red blood cells in the Tibetan high-altitude polycythemia population.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Wu,Zhijuan Liu,Doudou Hao,Qin Zhao,Wanjing Li,Maodi Xie,Xia Feng,Xia Liao,Siyuan Chen,Siyu Wang,Chaohua Zhou,Wenchun Long,Yajun Zhong,Shen Li,Ye Cao,Hong Wang,Aiping Wang,Yuehong Xu,Min Huang,Jiaxin Liu,Rui Zhong,Yunhong Wu,Zeng He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424]

Western blot - Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (AB125070)

Western blot - Anti-Band 3/AE 1 (phospho Y21) antibody [EPR1424] (AB125070)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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