AB231331

Anti-BANF1/BAF antibody

Name: Anti-BANF1/BAF antibody (ab231331) | Abcam
Brand: Abcam Limited
SKU: AB231331
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal BANF1/BAF antibody. Suitable for WB, IHC-P and reacts with Rat, Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BANF1.

View Alternative Names

BAF, BCRG1, BANF1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for BANF1/BAF using ab231331 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Formalin-fixed, paraffin-embedded human glioma tissue stained for BANF1/BAF using ab231331 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Formalin-fixed, paraffin-embedded human skin cancer tissue stained for BANF1/BAF using ab231331 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Formalin-fixed, paraffin-embedded human stomach tissue stained for BANF1/BAF using ab231331 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for BANF1/BAF using ab231331 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Formalin-fixed, paraffin-embedded human kidney tissue stained for BANF1/BAF using ab231331 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-BANF1/BAF antibody (AB231331)

All lanes:

Western blot - Anti-BANF1/BAF antibody (ab231331) at 2 µg/mL

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HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 10 kDa

false

Supplier Data

Western blot - Anti-BANF1/BAF antibody (AB231331)

All lanes:

Western blot - Anti-BANF1/BAF antibody (ab231331) at 1 µg/mL

All lanes:

Rat testis lysate

Predicted band size: 10 kDa

false

Supplier Data

Western blot - Anti-BANF1/BAF antibody (AB231331)

All lanes:

Western blot - Anti-BANF1/BAF antibody (ab231331) at 2 µg/mL

All lanes:

Recombinant human BANF1/BAF protein

Predicted band size: 10 kDa

false

Supplier Data

Western blot - Anti-BANF1/BAF antibody (AB231331)

All lanes:

Western blot - Anti-BANF1/BAF antibody (ab231331) at 2 µg/mL

All lanes:

Pig skeletal muscle lysate

Predicted band size: 10 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BANF1.

O75531

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA (PubMed : 10908652, PubMed : 11792822, PubMed : 12163470, PubMed : 18005698, PubMed : 25991860, PubMed : 28841419, PubMed : 31796734, PubMed : 32792394). Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging (PubMed : 9465049). Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner (PubMed : 28841419). Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface (PubMed : 28841419). Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity (PubMed : 32792394). Outcompetes CGAS for DNA-binding, thereby preventing CGAS activation and subsequent damaging autoinflammatory responses (PubMed : 32792394). Also involved in DNA damage response : interacts with PARP1 in response to oxidative stress, thereby inhibiting the ADP-ribosyltransferase activity of PARP1 (PubMed : 31796734). Involved in the recognition of exogenous dsDNA in the cytosol : associates with exogenous dsDNA immediately after its appearance in the cytosol at endosome breakdown and is required to avoid autophagy (PubMed : 25991860). In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication (PubMed : 18005698).. (Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome (PubMed : 11005805, PubMed : 16680152, PubMed : 9465049). EMD and BAF are cooperative cofactors of HIV-1 infection (PubMed : 16680152). Association of EMD with the viral DNA requires the presence of BAF and viral integrase (PubMed : 16680152). The association of viral DNA with chromatin requires the presence of BAF and EMD (PubMed : 16680152).

See full target information BANF1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of developmental biology 11: PubMed38132712

2023

Special Nuclear Structures in the Germinal Vesicle of the Common Frog with Emphasis on the So-Called Karyosphere Capsule.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitry S Bogolyubov,Sergey V Shabelnikov,Alexandra O Travina,Maksim I Sulatsky,Irina O Bogolyubova

Frontiers in oncology 12:875097 PubMed35586489

2022

ACYP1 Is a Pancancer Prognostic Indicator and Affects the Immune Microenvironment in LIHC.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyi Zhou,Zheng Fu,Shuai Wang,Jing Jia,Yumeng Cheng,Yunxiang Zheng,Ningning Zhang,Wei Lu,Zhi Yao

Oncology letters 20:1163-1170 PubMed32724356

2020

Downregulation of VRK1 reduces the expression of BANF1 and suppresses the proliferative and migratory activity of esophageal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Ren,Jie Geng,Chao Xiong,Xuebing Li,Yuqing Li,Jin Li,Hongchun Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BANF1/BAF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

Western blot - Anti-BANF1/BAF antibody (AB231331)

Western blot - Anti-BANF1/BAF antibody (AB231331)

Western blot - Anti-BANF1/BAF antibody (AB231331)

Western blot - Anti-BANF1/BAF antibody (AB231331)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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