JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB129074

Anti-BANF1/BAF antibody [EPR7669]

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal BANF1/BAF antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
3 Images
Flow Cytometry (Intracellular) - Anti-BANF1/BAF antibody [EPR7669] (AB129074)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-BANF1/BAF antibody [EPR7669] (AB129074)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling BANF1/BAF with Purified ab129074 at 1 : 20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7669] (AB129074)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7669] (AB129074)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab129074 at 1 : 50 dilution (6.0 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-BANF1/BAF antibody [EPR7669] (AB129074)
  • WB

Unknown

Western blot - Anti-BANF1/BAF antibody [EPR7669] (AB129074)

All lanes:

Western blot - Anti-BANF1/BAF antibody [EPR7669] (ab129074) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

false

  • Carrier free

    Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free

  • 660 APC

    APC Anti-BANF1/BAF antibody [EPR7669]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-BANF1/BAF antibody [EPR7669]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-BANF1/BAF antibody [EPR7669]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-BANF1/BAF antibody [EPR7669]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-BANF1/BAF antibody [EPR7669]

  • 578 PE

    PE Anti-BANF1/BAF antibody [EPR7669]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7669

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB231331

Anti-BANF1/BAF antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1/BAF antibody (AB231331)

  • 0
  • 0
Primary Antibodies

AB208532

Alexa Fluor® 594 Anti-BANF1/BAF antibody [EPR7668]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-BANF1/BAF antibody [EPR7668] (AB208532)

  • 0
  • 0
Primary Antibodies

AB129184

Anti-BANF1/BAF antibody [EPR7668]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] (AB129184)

  • 5
  • 6
Primary Antibodies

AB208621

Alexa Fluor® 647 Anti-BANF1/BAF antibody [EPR7668]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-BANF1/BAF antibody [EPR7668] (AB208621)

  • 0
  • 0
Primary Antibodies

AB240953

Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7668] - BSA and Azide free (AB240953)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB133741

Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (AB133741)

  • 4
  • 15
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA (PubMed : 10908652, PubMed : 11792822, PubMed : 12163470, PubMed : 18005698, PubMed : 25991860, PubMed : 28841419, PubMed : 31796734, PubMed : 32792394). Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging (PubMed : 9465049). Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner (PubMed : 28841419). Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface (PubMed : 28841419). Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity (PubMed : 32792394). Outcompetes CGAS for DNA-binding, thereby preventing CGAS activation and subsequent damaging autoinflammatory responses (PubMed : 32792394). Also involved in DNA damage response : interacts with PARP1 in response to oxidative stress, thereby inhibiting the ADP-ribosyltransferase activity of PARP1 (PubMed : 31796734). Involved in the recognition of exogenous dsDNA in the cytosol : associates with exogenous dsDNA immediately after its appearance in the cytosol at endosome breakdown and is required to avoid autophagy (PubMed : 25991860). In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication (PubMed : 18005698).. (Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome (PubMed : 11005805, PubMed : 16680152, PubMed : 9465049). EMD and BAF are cooperative cofactors of HIV-1 infection (PubMed : 16680152). Association of EMD with the viral DNA requires the presence of BAF and viral integrase (PubMed : 16680152). The association of viral DNA with chromatin requires the presence of BAF and EMD (PubMed : 16680152).
See full target information BANF1

Alternative Names

BAF, BCRG1, BANF1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full listand refine your search

Frontiers in oncology 12:975088 PubMed36185250

2022

Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cox,Daniel Nierenberg,Oscar Camargo,Eunkyung Lee,Amr S Khaled,Joseph Mazar,Rebecca J Boohaker,Tamarah J Westmoreland,Annette R Khaled
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com