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AB248281

Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal BANF1/BAF antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

BAF, BCRG1, BANF1, Barrier-to-autointegration factor, Breakpoint cluster region protein 1

3 Images
Flow Cytometry (Intracellular) - Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (AB248281)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (AB248281)

This data was developed using ab248281, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling BANF1/BAF with Purified ab248281 at 1 : 20 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (AB248281)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (AB248281)

This data was developed using ab248281, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab248281 at 1 : 50 dilution (6.0 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (AB248281)
  • WB

Unknown

Western blot - Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (AB248281)

All lanes:

Western blot - Anti-BANF1/BAF antibody [EPR7669] (<a href='/en-us/products/primary-antibodies/banf1-baf-antibody-epr7669-ab129074'>ab129074</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

false

  • Unconjugated

    Anti-BANF1/BAF antibody [EPR7669]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-BANF1/BAF antibody [EPR7669]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-BANF1/BAF antibody [EPR7669]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-BANF1/BAF antibody [EPR7669]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-BANF1/BAF antibody [EPR7669]

  • 578 PE

    PE Anti-BANF1/BAF antibody [EPR7669]

  • 660 APC

    APC Anti-BANF1/BAF antibody [EPR7669]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7669

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248281 is the carrier-free version of ab129074.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-specific DNA-binding protein that plays key roles in mitotic nuclear reassembly, chromatin organization, DNA damage response, gene expression and intrinsic immunity against foreign DNA (PubMed : 10908652, PubMed : 11792822, PubMed : 12163470, PubMed : 18005698, PubMed : 25991860, PubMed : 28841419, PubMed : 31796734, PubMed : 32792394). Contains two non-specific double-stranded DNA (dsDNA)-binding sites which promote DNA cross-bridging (PubMed : 9465049). Plays a key role in nuclear membrane reformation at the end of mitosis by driving formation of a single nucleus in a spindle-independent manner (PubMed : 28841419). Transiently cross-bridges anaphase chromosomes via its ability to bridge distant DNA sites, leading to the formation of a dense chromatin network at the chromosome ensemble surface that limits membranes to the surface (PubMed : 28841419). Also acts as a negative regulator of innate immune activation by restricting CGAS activity toward self-DNA upon acute loss of nuclear membrane integrity (PubMed : 32792394). Outcompetes CGAS for DNA-binding, thereby preventing CGAS activation and subsequent damaging autoinflammatory responses (PubMed : 32792394). Also involved in DNA damage response : interacts with PARP1 in response to oxidative stress, thereby inhibiting the ADP-ribosyltransferase activity of PARP1 (PubMed : 31796734). Involved in the recognition of exogenous dsDNA in the cytosol : associates with exogenous dsDNA immediately after its appearance in the cytosol at endosome breakdown and is required to avoid autophagy (PubMed : 25991860). In case of poxvirus infection, has an antiviral activity by blocking viral DNA replication (PubMed : 18005698).. (Microbial infection) Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome (PubMed : 11005805, PubMed : 16680152, PubMed : 9465049). EMD and BAF are cooperative cofactors of HIV-1 infection (PubMed : 16680152). Association of EMD with the viral DNA requires the presence of BAF and viral integrase (PubMed : 16680152). The association of viral DNA with chromatin requires the presence of BAF and EMD (PubMed : 16680152).
See full target information BANF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of developmental biology 11: PubMed38132712

2023

Special Nuclear Structures in the Germinal Vesicle of the Common Frog with Emphasis on the So-Called Karyosphere Capsule.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitry S Bogolyubov,Sergey V Shabelnikov,Alexandra O Travina,Maksim I Sulatsky,Irina O Bogolyubova
View all publications
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