Anti-BANK1 antibody [EPR20788] - BSA and Azide free

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left panel) / Daudi (human Burkitt's lymphoma lymphoblast, Right panel) cell lines labeling BANK1 with ab229190 at 1/400 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^� 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

The Jurkat cell line is a negative control for BANK1.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229190).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID : 24879116). Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229190).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492)

Immunohistochemical analysis of paraffin-embedded human Large B cell lymphoma tissue labeling BANK1 with ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human Large B Cell Lymphoma cells (PMID : 24879116) is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229190).