Rabbit Recombinant Monoclonal BANK1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492), expandable thumbnail](https://content.abcam.com/products/images/bank1-antibody-epr20788-bsa-and-azide-free-ab230492--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73378.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492), expandable thumbnail](https://content.abcam.com/products/images/bank1-antibody-epr20788-bsa-and-azide-free-ab230492--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50039.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492)")
![Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492), expandable thumbnail](https://content.abcam.com/products/images/bank1-antibody-epr20788-bsa-and-azide-free-ab230492--flow-cytometry-intracellular-img12838.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (AB230492)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Involved in B-cell receptor (BCR)-induced Ca(2+) mobilization from intracellular stores. Promotes Lyn-mediated phosphorylation of IP3 receptors 1 and 2.
Alternative names
B-cell scaffold protein with ankyrin repeats, BANK1
Recommended products
Rabbit Recombinant Monoclonal BANK1 antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20788
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab230492 is the carrier-free version of Anti-BANK1 antibody [EPR20788] ab229190.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492), expandable thumbnail](https://content.abcam.com/products/images/bank1-antibody-epr20788-bsa-and-azide-free-ab230492--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img73378.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling BANK1 with Anti-BANK1 antibody [EPR20788] ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining is weak in the germinal center but strong in the mantle zones of human tonsil (PMID: 24879116). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BANK1 antibody [EPR20788] ab229190).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492), expandable thumbnail](https://content.abcam.com/products/images/bank1-antibody-epr20788-bsa-and-azide-free-ab230492--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50039.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492)
Immunohistochemical analysis of paraffin-embedded human Large B cell lymphoma tissue labeling BANK1 with Anti-BANK1 antibody [EPR20788] ab229190 at 1/4000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human Large B Cell Lymphoma cells (PMID: 24879116) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BANK1 antibody [EPR20788] ab229190).
![Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492), expandable thumbnail](https://content.abcam.com/products/images/bank1-antibody-epr20788-bsa-and-azide-free-ab230492--flow-cytometry-intracellular-img12838.jpg "Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492)")
Flow Cytometry (Intracellular) - Anti-BANK1 antibody [EPR20788] - BSA and Azide free (ab230492)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left panel) / Daudi (human Burkitt's lymphoma lymphoblast, Right panel) cell lines labeling BANK1 with Anti-BANK1 antibody [EPR20788] ab229190 at 1/400 (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
The Jurkat cell line is a negative control for BANK1.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BANK1 antibody [EPR20788] ab229190).
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