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AB245391

Anti-BAP1 antibody

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(3 Publications)

Rabbit Polyclonal BAP1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BAP1 aa 650 to C-terminus.

View Alternative Names

KIAA0272, hucep-6, BAP1, Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody (AB245391)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody (AB245391)

Immunohistochemical analysis of formalin-fixed, parafifin-embedded human prostate carcinoma tissue labelling BAP1 with ab245391, followed by HRP-conjugated Goat Anti-Rabbit IgG. Substrate : DAB.

Immunoprecipitation - Anti-BAP1 antibody (AB245391)
  • IP

Supplier Data

Immunoprecipitation - Anti-BAP1 antibody (AB245391)

BAP1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).

ab245391 used for IP at 10 μg/mg lysate. For WB 1 μg/ml.

Lane 1 : ab245391 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-BAP1 antibody (ab245391)

Predicted band size: 80 kDa

false

Western blot - Anti-BAP1 antibody (AB245391)
  • WB

Supplier Data

Western blot - Anti-BAP1 antibody (AB245391)

All lanes:

Western blot - Anti-BAP1 antibody (ab245391) at 0.4 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 80 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human BAP1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q92560

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p>Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245391 was affinity purified using an epitope specific to BAP1 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed : 12485996, PubMed : 18757409, PubMed : 20436459, PubMed : 25451922, PubMed : 35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed : 20436459, PubMed : 25451922, PubMed : 30664650, PubMed : 35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed : 20436459, PubMed : 30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed : 20805357, PubMed : 30664650, PubMed : 36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed : 30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed : 36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed : 20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed : 19188440, PubMed : 19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed : 19188440, PubMed : 19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed : 19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed : 19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed : 24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed : 34170818).
See full target information BAP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:564-575 PubMed38449391

2024

The deubiquitinase BRCC3 increases the stability of ZEB1 and promotes the proliferation and metastasis of triple-negative breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qidi Huang,Shurong Zheng,Huayan Gu,Zhi Yang,Yiqiao Lu,Xia Yu,Guilong Guo

Oncogene 42:1843-1856 PubMed37081042

2023

De-ubiquitination of SAMHD1 by USP7 promotes DNA damage repair to overcome oncogenic stress and affect chemotherapy sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Liu,Tingting Zhou,Xiang Dong,Qiqiang Guo,Lixia Zheng,Xiaoxun Wang,Naijin Zhang,Danni Li,Ling Ren,Fei Yi,Ying Zhang,Ziwei Li,Xiwen Wang,Chengsi Deng,Chunlu Li,Hongde Xu,Yi Guan,Xiaoman Li,Yang Yu,Wendong Guo,Zhuo Wang,Bo Jiang,Xuan Wu,Ning Bai,Yanling Feng,Mengtao Ma,Qingquan Kong,Jiayi Wei,Zhenshuang Wang,Hao Li,Songming Lu,Liangzi Cao,Yutong Xiao,Xiaoyu Song,Zhenning Wang,Chengzhong Xing,Liu Cao

FEBS open bio : PubMed33529461

2021

BAP1 promotes viability and migration of ECA109 cells through KLF5/CyclinD1/FGF-BP1.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyun Wang,Ming Luo,Honglan Qu,Yufeng Cheng
View all publications
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