Anti-BAP1 antibody [EPR22826-65]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling BAP1 with ab255611 at 1/60 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma labelling BAP1 with ab255611 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab255611 anti-BAP1 antibody [EPR22826-65] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human lung carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human colon carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on rat cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on mouse cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

Lab

Western blot - Anti-BAP1 antibody [EPR22826-65] (AB255611)

The molecular weight observed is consistent with what has been described in the literature (PMID : 18757409).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

A375 (human malignant melanoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 80 kDa

Observed band size: 100 kDa

false

Exposure time: 3min