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Anti-BAP1 antibody [EPR22826-65] ab255611 is a rabbit monoclonal antibody that is used in BAP1 western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Validated on the Leica BOND™ RX automated IHC staining platform for IHC
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (AB255611), expandable thumbnail
  • Western blot - Anti-BAP1 antibody [EPR22826-65] (AB255611), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Not recommended
Tested
Mouse
Not recommended
Expected
Tested
Not recommended
Expected
Rat
Not recommended
Expected
Tested
Not recommended
Expected

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/60
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1 (PubMed:12485996, PubMed:18757409, PubMed:20436459, PubMed:25451922, PubMed:35051358). Catalytic component of the polycomb repressive deubiquitinase (PR-DUB) complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-120' (H2AK119ub1) (PubMed:20436459, PubMed:25451922, PubMed:30664650, PubMed:35051358). Does not deubiquitinate monoubiquitinated histone H2B (PubMed:20436459, PubMed:30664650). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed:20805357, PubMed:30664650, PubMed:36180891). Antagonizes PRC1 mediated H2AK119ub1 monoubiquitination (PubMed:30664650). As part of the PR-DUB complex, associates with chromatin enriched in histone marks H3K4me1, H3K4me3, and H3K27Ac, but not in H3K27me3 (PubMed:36180891). Recruited to specific gene-regulatory regions by YY1 (PubMed:20805357). Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains (PubMed:19188440, PubMed:19815555). Deubiquitination of HCFC1 does not lead to increase stability of HCFC1 (PubMed:19188440, PubMed:19815555). Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination (PubMed:19117993). It however does not mediate deubiquitination of BRCA1 and BARD1 (PubMed:19117993). Able to mediate autodeubiquitination via intramolecular interactions to counteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed:34170818).

Alternative names

KIAA0272, hucep-6, BAP1, Ubiquitin carboxyl-terminal hydrolase BAP1, BRCA1-associated protein 1, Cerebral protein 6

Recommended products

Anti-BAP1 antibody [EPR22826-65] ab255611 is a rabbit monoclonal antibody that is used in BAP1 western blotting, IHC and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Validated on the Leica BOND™ RX automated IHC staining platform for IHC
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22826-65
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on rat cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on mouse cerebrum. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human lung carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue labelling BAP1 with ab255611 at 1/100 dilution. Heat mediated antigen retrieval was performed usingTris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins, LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Nuclear staining on human colon carcinoma. The section was incubated with ab255611 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611), expandable thumbnail

    Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611)

    The molecular weight observed is consistent with what has been described in the literature (PMID: 18757409).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-BAP1 antibody [EPR22826-65] (ab255611) at 1/1000 dilution

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: A375 (human malignant melanoma epithelial cell), whole cell lysate at 20 µg

    Lane 5: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 6: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 80 kDa

    Observed band size: 100 kDa

    Exposure time: 3min

  • Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BAP1 antibody [EPR22826-65] (ab255611)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized PC-3 (human prostate adenocarcinoma epithelial cell) cells labeling BAP1 with ab255611 at 1/60 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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