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AB129023

Anti-BAP29 antibody [EPR7781]

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(1 Publication)

Rabbit Recombinant Monoclonal BAP29 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BAP29, BCAP29, B-cell receptor-associated protein 29, BCR-associated protein 29, Bap29

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-BAP29 antibody [EPR7781] (AB129023)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BAP29 antibody [EPR7781] (AB129023)

ab129023, at 1/100, staining BAP29 in Hela cells by immunofluorescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP29 antibody [EPR7781] (AB129023)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP29 antibody [EPR7781] (AB129023)

ab129023 at 1/100 dilution staining BAP29 in formalin fixed paraffin embedded Human brain tissue by immunohistochemistry

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-BAP29 antibody [EPR7781] (AB129023)
  • WB

Unknown

Western blot - Anti-BAP29 antibody [EPR7781] (AB129023)

All lanes:

Western blot - Anti-BAP29 antibody [EPR7781] (ab129023) at 1/10000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

Fetal heart tissue lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

SW480 cell lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Predicted band size: 28 kDa

false

OI-RD Scanning - Anti-BAP29 antibody [EPR7781] (AB129023)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-BAP29 antibody [EPR7781] (AB129023)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7781

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis (By similarity).
See full target information BCAP29
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70236 PubMed39652089

2024

Corneal strain influences keratocyte proliferation and migration through upregulation of ALDH3A1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Xin Zhou,Wei Zhang,Xiaolei Wang,Shengqian Dou,Leilei Zhao,Roine El-Habta,Qingjun Zhou,Ludvig J Backman,Patrik Danielson
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com