Anti-BAP29 antibody [EPR7781] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BAP29 antibody [EPR7781] - BSA and Azide free (AB248268)

This data was developed using ab129023, the same antibody clone in a different buffer formulation.ab129023, at 1/100, staining BAP29 in Hela cells by immunofluorescence.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP29 antibody [EPR7781] - BSA and Azide free (AB248268)

This data was developed using ab129023, the same antibody clone in a different buffer formulation.

ab129023, at 1/100 dilution staining BAP29 in formalin fixed paraffin embedded Human brain tissue by immunohistochemistry

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-BAP29 antibody [EPR7781] - BSA and Azide free (AB248268)

This data was developed using ab129023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BAP29 antibody [EPR7781] (<a href='/en-us/products/primary-antibodies/bap29-antibody-epr7781-ab129023'>ab129023</a>) at 1/10000 dilution

Lane 1:

Fetal brain tissue lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

Fetal heart tissue lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Lane 5:

SW480 cell lysate at 10 µg

Lane 6:

HeLa cell lysate at 10 µg

Predicted band size: 28 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-BAP29 antibody [EPR7781] - BSA and Azide free (AB248268)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.