Anti-BAP31 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (AB237485)

Paraffin-embedded human colon cancer tissue stained for BAP31 using ab237485 at 1/1200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (AB237485)

Paraffin-embedded human prostate cancer tissue stained for BAP31 using ab237485 at 1/1200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody (AB237485)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for BAP31 (green) using ab237485 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L).

• IP

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Immunoprecipitation - Anti-BAP31 antibody (AB237485)

BAP31 was immunoprecipitated from mouse liver tissue lysate using ab237485 at 1/200 dilution.

Lane 1 : Rabbit control IgG in mouse liver tissue lysate.

Lane 2 : ab237485 in mouse liver tissue lysate.

Lane 3 : Mouse liver tissue lysate (10 μg input).

For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.

All lanes:

Immunoprecipitation - Anti-BAP31 antibody (ab237485)

Predicted band size: 28 kDa

false

• WB

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Western blot - Anti-BAP31 antibody (AB237485)

All lanes:

Western blot - Anti-BAP31 antibody (ab237485) at 1/1000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Lane 5:

Rat liver tissue lysate

Lane 6:

Rat brain tissue lysate

Lane 7:

Mouse liver tissue lysate

Lane 8:

Mouse brain tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 28 kDa

false