AB109304

Anti-BAP31 antibody [EPR3878(2)]

Name: Anti-BAP31 antibody [EPR3878(2)] (ab109304) | Abcam
Brand: Abcam Limited
SKU: AB109304
Price: 340 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal BAP31 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications.

View Alternative Names

BAP31, DXS1357E, BCAP31, B-cell receptor-associated protein 31, BCR-associated protein 31, Bap31, 6C6-AG tumor-associated antigen, Protein CDM, p28

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

ab109304 at 1/250 dilution staining Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

ab109304 at 1/250 dilution staining Human brain tissue by immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

ab109304, at 1/100 dilution, staining HeLa cells by immunofluorescence.

Unknown

Western blot - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

All lanes:

Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Human placenta tissue lysate at 10 µg

Lane 3:

NCCIT cell lysate at 10 µg

Predicted band size: 28 kDa

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Lab

Western blot - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

Lanes 1-4 : Merged signal (red and green). Green - ab109304 observed at 28 kDa. Red - loading control ab7291 observed at 50 kDa.

ab109304 Anti-BAP31 antibody [EPR3878(2)] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab109304 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

BCAP31 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human BCAP31 (BAP31) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-bcap31-bap31-knockout-hek-293t-cell-line-ab266634'>ab266634</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

NCCIT cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 28 kDa

false

Carrier free

Anti-BAP31 antibody [EPR3878(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3878(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a chaperone protein (PubMed : 18287538, PubMed : 9396746). Is one of the most abundant endoplasmic reticulum (ER) proteins (PubMed : 18287538, PubMed : 9396746). Plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD) (PubMed : 18287538, PubMed : 9396746). Also serves as a cargo receptor for the export of transmembrane proteins (By similarity). Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by stimulating the translocation of NDUFS4 and NDUFB11 from the cytosol to the mitochondria via interaction with TOMM40 (PubMed : 31206022). In response to ER stress, delocalizes from the ER-mitochondria contact sites and binds BCL2 (PubMed : 31206022). May be involved in CASP8-mediated apoptosis (PubMed : 10958671).

See full target information BCAP31

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e29291 PubMed38644851

2024

Melatonin inhibits tongue squamous cell carcinoma: Interplay of ER stress-induced apoptosis and autophagy with cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Liu,Ye Zheng,Shaoning Kan,Ming Hao,Huan Jiang,Shuangji Li,Rong Li,Yinyu Wang,Dongxu Wang,Weiwei Liu

Cancer letters 571:216333 PubMed37543278

2023

A novel GRK3-HDAC2 regulatory pathway is a key direct link between neuroendocrine differentiation and angiogenesis in prostate cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Naderinezhad,Guoliang Zhang,Zheng Wang,Dayong Zheng,Mohit Hulsurkar,Michael Bakhoum,Ning Su,Han Yang,Tao Shen,Wenliang Li

Proceedings of the National Academy of Sciences of the United States of America 120:e2219790120 PubMed37307450

2023

Dynamic interactome of the MHC I peptide loading complex in human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Barends,Nicole Koller,Christian Schölz,Verónica Durán,Berislav Bošnjak,Jennifer Becker,Marius Döring,Hanna Blees,Reinhold Förster,Ulrich Kalinke,Robert Tampé

Cellular and molecular gastroenterology and hepatology 15:197-211 PubMed36122677

2022

Shc Is Implicated in Calreticulin-Mediated Sterile Inflammation in Alcoholic Hepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Li,Joy X Jiang,Weiguo Fan,Sarah R Fish,Suvarthi Das,Parul Gupta,Gergely Mozes,Lorand Vancza,Sutapa Sarkar,Koshi Kunimoto,Dongning Chen,Hyesuk Park,Dahn Clemens,Alexey Tomilov,Gino Cortopassi,Natalie J Török

The EMBO journal 41:e108272 PubMed35211994

2022

Reduced ER-mitochondria connectivity promotes neuroblastoma multidrug resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Jorida Çoku,David M Booth,Jan Skoda,Madison C Pedrotty,Jennifer Vogel,Kangning Liu,Annette Vu,Erica L Carpenter,Jamie C Ye,Michelle A Chen,Peter Dunbar,Elizabeth Scadden,Taekyung D Yun,Eiko Nakamaru-Ogiso,Estela Area-Gomez,Yimei Li,Kelly C Goldsmith,C Patrick Reynolds,Gyorgy Hajnoczky,Michael D Hogarty

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BAP31 antibody [EPR3878(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

Immunocytochemistry/ Immunofluorescence - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

Western blot - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

Western blot - Anti-BAP31 antibody [EPR3878(2)] (AB109304)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (5)

Product promise