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AB50984

Anti-BARD1 antibody [2059C4a]

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(5 Publications)

Mouse Monoclonal BARD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BARD1.

View Alternative Names

BRCA1-associated RING domain protein 1, BARD-1, RING-type E3 ubiquitin transferase BARD1, BARD1

2 Images
Western blot - Anti-BARD1 antibody [2059C4a] (AB50984)
  • WB

Unknown

Western blot - Anti-BARD1 antibody [2059C4a] (AB50984)

All lanes:

Western blot - Anti-BARD1 antibody [2059C4a] (ab50984) at 1/50 dilution

All lanes:

HEK293 whole cell lysate at 50 µg

Secondary

All lanes:

Anti mouse IgG antibody at 1/2500 dilution

Predicted band size: 87 kDa

Observed band size: 100 kDa

false

Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (AB50984)
  • IP

Unknown

Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (AB50984)

ab50984 at 1/50 dilution precipitating RIPA lysate of HeLa cells at 500μg; visualised with anti mouse IgG antibody at 1/2500 diltuion.

All lanes:

Immunoprecipitation - Anti-BARD1 antibody [2059C4a] (ab50984)

Predicted band size: 87 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2059C4a

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human BARD1. The exact immunogen used to generate this antibody is proprietary information.

Q99728

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Protein G purified from culture of supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (~95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
See full target information BARD1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 83:3679-3691.e8 PubMed37797621

2023

Crucial roles of the BRCA1-BARD1 E3 ubiquitin ligase activity in homology-directed DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Meiling Wang,Wenjing Li,Nozomi Tomimatsu,Corey H Yu,Jae-Hoon Ji,Salvador Alejo,Samuel R Witus,Dauren Alimbetov,O'Taveon Fitzgerald,Bo Wu,Qijing Wang,Yuxin Huang,Yaqi Gan,Felix Dong,Youngho Kwon,Gangadhara R Sareddy,Tyler J Curiel,Amyn A Habib,Robert Hromas,Carolina Dos Santos Passos,Tingting Yao,Dmitri N Ivanov,Peter S Brzovic,Sandeep Burma,Rachel E Klevit,Weixing Zhao

The EMBO journal 42:e113565 PubMed37305927

2023

BRCA1/BARD1 intrinsically disordered regions facilitate chromatin recruitment and ubiquitylation.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel R Witus,Lisa M Tuttle,Wenjing Li,Alex Zelter,Meiling Wang,Klaiten E Kermoade,Damien B Wilburn,Trisha N Davis,Peter S Brzovic,Weixing Zhao,Rachel E Klevit

Cancer research communications 2:220-232 PubMed36187937

2022

BRCA1-associated RING domain-1 (BARD1) loss and GBP1 expression enhance sensitivity to DNA damage in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa M Maurer,Jessica D Daley,Elina Mukherjee,Rosemarie E Venier,Claire M Julian,Nathanael G Bailey,Michelle F Jacobs,Chandan Kumar-Sinha,Haley Raphael,Nivitha Periyapatna,Kurt Weiss,Katherine A Janeway,Rajen Mody,Peter C Lucas,Linda M McAllister-Lucas,Kelly M Bailey

Cell biology international 46:922-932 PubMed35143098

2022

USP25 inhibits DNA damage by stabilizing BARD1 protein in a house dust mite-induced asthmatic model in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Gan,Ye Wang,Qian Zhao,Mi Kong,Jinnan Chen,Wanying Zhang,Lingxiao Tan,Man Tian

Proceedings of the National Academy of Sciences of 109:13650-5 PubMed22869732

2012

BRCA1 and HSP90 cooperate in homologous and non-homologous DNA double-strand-break repair and G2/M checkpoint activation.

Applications

WB

Species

Unspecified reactive species

Shane R Stecklein,Easwari Kumaraswamy,Fariba Behbod,Wenjia Wang,Vamsee Chaguturu,Lisa M Harlan-Williams,Roy A Jensen
View all publications
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