Rabbit Polyclonal BARD1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BARD1 aa 250-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BARD1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q99728
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Alternative names
BRCA1-associated RING domain protein 1, BARD-1, RING-type E3 ubiquitin transferase BARD1, BARD1
Recommended products
Rabbit Polyclonal BARD1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human BARD1 aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BARD1 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q99728
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab245434 was affinity purified using an epitope specific to BARD1 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-BARD1 antibody (ab245434)
All lanes: Western blot - Anti-BARD1 antibody (ab245434) at 1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Predicted band size: 87 kDa
Exposure time: 30s
Immunoprecipitation - Anti-BARD1 antibody (ab245434)
BARD1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245434 used for IP at 6 μg/mg lysate. For WB 1 μg/ml.
Lane 1: ab245434 (Lot 2) IP in HeLa whole cell lysate.
Lane 2: ab245434 (Lot 3) IP in HeLa whole cell lysate.
Lane 3: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 3 minutes.All lanes: Immunoprecipitation - Anti-BARD1 antibody (ab245434)
Predicted band size: 87 kDa
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