JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB181851

Anti-BarX1 antibody [EPR14120]

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Recombinant Monoclonal BARX1 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Homeobox protein BarH-like 1, BARX1

1 Images
Western blot - Anti-BarX1 antibody [EPR14120] (AB181851)
  • WB

Lab

Western blot - Anti-BarX1 antibody [EPR14120] (AB181851)

Diluting/Blocking buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-BarX1 antibody [EPR14120] (ab181851) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) nuclear fraction lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) without nuclear fraction lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14120

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BarX1 also known as BarH-like homeobox 1 is a transcription factor with a molecular mass of approximately 29 kDa. It is part of the Bar family of proteins which play essential roles in development. BarX1's primary expression occurs in the central nervous system and mesenchymal tissues during embryonic development. In adults BarX1 shows limited expression in specific tissues such as the stomach where it may serve various regulatory functions.
Biological function summary

BarX1 functions in the development of multiple organ systems particularly in teeth and stomach. It acts by influencing the differentiation and proliferation of cells during the formation of these structures. BarX1 is known to be part of a regulatory complex that controls the expression of genes critical for morphogenesis and tissue specification. Its interaction with other homeobox proteins further expands its impact on development.

Pathways

BarX1 interacts in key developmental signaling pathways such as Wnt and Hedgehog. These pathways play significant roles in development influencing cell fate and patterning. BarX1's interaction with proteins like beta-Catenin in the Wnt pathway helps modulate the activity of downstream genes necessary for development. By participating in these pathways BarX1 helps maintain the balance between cell proliferation and differentiation.

Dysregulation of BarX1 expression has been linked to conditions such as gastric cancer and tooth agenesis. In gastric cancer abnormal BarX1 levels can disrupt normal gene expression leading to uncontrolled cell growth and tumor development. Connections also exist between BarX1 and E-cadherin; alterations in this interaction can influence cancer progression. In tooth agenesis insufficient BarX1 activity can impair the formation of dental tissues resulting in missing teeth. Understanding these associations aids in developing targeted therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor, which is involved in craniofacial development, in odontogenesis and in stomach organogenesis. May have a role in the differentiation of molars from incisors. Plays a role in suppressing endodermal Wnt activity (By similarity). Binds to a regulatory module of the NCAM promoter.
See full target information Homeobox protein BarH-like 1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biochemical genetics 63:5394-5414 PubMed39656403

2024

Inflammatory Signaling Induces Mitochondrial Dysfunction and Neuronal Death in Traumatic Brain Injury via Downregulation of OXPHOS Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Dong,Hui Zhang,Lei Cai,Quanyi Ye,Heping Wang,Bo Liu,Wenhu Zhang,Junxin Li

Cell death & disease 14:527 PubMed37587140

2023

ZFP36 loss-mediated BARX1 stabilization promotes malignant phenotypes by transactivating master oncogenes in NSCLC.

Applications

Unspecified application

Species

Unspecified reactive species

Tongjia Zhang,Lizhen Qiu,Jiashun Cao,Qiu Li,Lifan Zhang,Guoshun An,Juhua Ni,Hongti Jia,Shuyan Li,Kailong Li

Cell proliferation 52:e12522 PubMed30443989

2018

AP2a enhanced the osteogenic differentiation of mesenchymal stem cells by inhibiting the formation of YAP/RUNX2 complex and BARX1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Lin,Haoqing Yang,Lijun Wang,Wenzhi Li,Shu Diao,Juan Du,Songlin Wang,Rui Dong,Jun Li,Zhipeng Fan

Cell death and differentiation 25:1996-2009 PubMed29523871

2018

GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Guo,Yuxin Zhang,Tingting Zhou,Dongyue Wang,Yajuan Weng,Qi Chen,Junqing Ma,Yi-Ping Li,Lin Wang

Cancer letters 421:103-111 PubMed29454095

2018

An esophageal adenocarcinoma susceptibility locus at 9q22 also confers risk to esophageal squamous cell carcinoma by regulating the function of BARX1.

Applications

WB

Species

Unspecified reactive species

Caiwang Yan,Yong Ji,Tongtong Huang,Fei Yu,Yong Gao,Yayun Gu,Qi Qi,Jiangbo Du,Juncheng Dai,Hongxia Ma,Guangfu Jin
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB220859

Anti-BarX1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-BarX1 antibody (AB220859)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB46154

Anti-VEGFA antibody

Lab Essentials

Western blot - Anti-VEGFA antibody (AB46154)

  • 5
  • 29
Primary Antibodies

AB305065

Anti-Plzf antibody [EPR24830-8] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Plzf antibody [EPR24830-8] - BSA and Azide free (AB305065)

  • 0
  • 0
Primary Antibodies

AB179810

Anti-LOXL2 antibody [EPR12733] - C-terminal

RabMAb|Recombinant|KO Validated

Western blot - Anti-LOXL2 antibody [EPR12733] - C-terminal (AB179810)

  • 4
  • 6
Primary Antibodies

AB70788

Anti-RCC2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RCC2 antibody (AB70788)

  • 5
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com