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AB250523

Anti-BarX1 antibody [EPR14120] - BSA and Azide free

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Rabbit Recombinant Monoclonal BARX1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Homeobox protein BarH-like 1, BARX1

1 Images
Western blot - Anti-BarX1 antibody [EPR14120] - BSA and Azide free (AB250523)
  • WB

Lab

Western blot - Anti-BarX1 antibody [EPR14120] - BSA and Azide free (AB250523)

This data was developed using ab181851, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BarX1 antibody [EPR14120] - BSA and Azide free (ab250523) at 1/1000 dilution

Lane 1:

HepG2 (Human hepatocellular carcinoma epithelial cell) nuclear fraction lysate at 20 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) without nuclear fraction lysate at 20 µg

Lane 3:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 28 kDa

Observed band size: 32 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14120

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250523 is the carrier-free version of ab181851.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BarX1 also known as BarH-like homeobox 1 is a transcription factor with a molecular mass of approximately 29 kDa. It is part of the Bar family of proteins which play essential roles in development. BarX1's primary expression occurs in the central nervous system and mesenchymal tissues during embryonic development. In adults BarX1 shows limited expression in specific tissues such as the stomach where it may serve various regulatory functions.
Biological function summary

BarX1 functions in the development of multiple organ systems particularly in teeth and stomach. It acts by influencing the differentiation and proliferation of cells during the formation of these structures. BarX1 is known to be part of a regulatory complex that controls the expression of genes critical for morphogenesis and tissue specification. Its interaction with other homeobox proteins further expands its impact on development.

Pathways

BarX1 interacts in key developmental signaling pathways such as Wnt and Hedgehog. These pathways play significant roles in development influencing cell fate and patterning. BarX1's interaction with proteins like beta-Catenin in the Wnt pathway helps modulate the activity of downstream genes necessary for development. By participating in these pathways BarX1 helps maintain the balance between cell proliferation and differentiation.

Dysregulation of BarX1 expression has been linked to conditions such as gastric cancer and tooth agenesis. In gastric cancer abnormal BarX1 levels can disrupt normal gene expression leading to uncontrolled cell growth and tumor development. Connections also exist between BarX1 and E-cadherin; alterations in this interaction can influence cancer progression. In tooth agenesis insufficient BarX1 activity can impair the formation of dental tissues resulting in missing teeth. Understanding these associations aids in developing targeted therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor, which is involved in craniofacial development, in odontogenesis and in stomach organogenesis. May have a role in the differentiation of molars from incisors. Plays a role in suppressing endodermal Wnt activity (By similarity). Binds to a regulatory module of the NCAM promoter.
See full target information Homeobox protein BarH-like 1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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