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AB155577

Anti-Basophils antibody [2D7]

4

(6 Reviews)

|

(7 Publications)

Mouse Monoclonal Basophils antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Cell preparation containing Basophils protein.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D7

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB, IHC-Fr, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 99% PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Basophils known alternatively as 'basophil granulocytes' are a type of white blood cell with a mass around 0.6 picograms. You find these cells in the bloodstream where they are released from the bone marrow. They have a low abundance in peripheral blood typically accounting for about 0.1% of white blood cells. Basophils express surface markers such as CD123 and the high-affinity receptor for IgE (FcεRI). They are rich in granules that contain heparin histamine and various cytokines.
Biological function summary

Basophils play an important role in immune response regulation particularly in allergic reactions and parasitic infections. Basophils do not form part of a complex but interact dynamically with other immune cells. Released substances from their granules contribute to inflammation and help control the intensity of the immune response. Basophil markers including 2D7 help identify these cells and examine their function in immunological studies.

Pathways

Basophils are critical players in the Th2 immune response pathway and the allergic inflammation pathway. In the Th2 response basophils secrete cytokines that promote the activation and differentiation of T-helper 2 (Th2) cells. They relate to other proteins like IL-4 and IL-13 which are significant in the mediation of allergic responses. Basophils contribute to the recruitment and activation of other immune cells like eosinophils through the secretion of cytokines and chemokines.

Basophils are significant contributors to conditions such as asthma and systemic anaphylaxis. They have been connected to the pathophysiology of these diseases through their role in mediating excessive allergic reactions. Basophils interact closely with proteins such as histamine amplifying the symptoms during these hypersensitivity conditions. Understanding basophil markers has improved diagnostic and therapeutic strategies in managing allergic diseases.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The Journal of dermatology 50:927-930 PubMed36651089

2023

IL-31 and IL-31 receptor alpha in pemphigus: Contributors to more than just itch?

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Okuno,Takashi Hashimoto,Yuki Yamazaki,Manami Okuzawa,Takahiro Satoh

Journal of the American Academy of Dermatology 83:53-62 PubMed31351883

2019

Pathophysiologic mechanisms of itch in bullous pemphigoid.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Hashimoto,Christina Dorothy Kursewicz,Rachel Alison Fayne,Sonali Nanda,Serena Maya Shah,Leigh Nattkemper,Hiroo Yokozeki,Gil Yosipovitch

Journal of gastroenterology 53:449-460 PubMed28921377

2017

Basophils activated via TLR signaling may contribute to pathophysiology of type 1 autoimmune pancreatitis.

Applications

IHC-P

Species

Human

Masato Yanagawa,Kazushige Uchida,Yugo Ando,Takashi Tomiyama,Takashi Yamaguchi,Tsukasa Ikeura,Toshiro Fukui,Akiyoshi Nishio,Yoshiko Uemura,Takayuki Miyara,Hiroyuki Okamoto,Souhei Satoi,Kazuichi Okazaki

PloS one 9:e111352 PubMed25361058

2014

MBP-positive and CD11c-positive cells are associated with different phenotypes of Korean patients with non-asthmatic chronic rhinosinusitis.

Applications

IHC

Species

Human

Dong-Kyu Kim,Min-Hyun Park,Dong-Yeop Chang,Kyung Mi Eun,Hyun-Woo Shin,Ji-Hun Mo,Eui-Cheol Shin,Hong Ryul Jin,Sue Shin,Eun Youn Roh,Doo Hee Han,Dae Woo Kim

Journal of clinical pathology 59:396-402 PubMed16461568

2006

Enumeration and immunohistochemical characterisation of bone marrow basophils in myeloproliferative disorders using the basophil specific monoclonal antibody 2D7.

Applications

Unspecified application

Species

Unspecified reactive species

H Agis,M-T Krauth,I Mosberger,L Müllauer,I Simonitsch-Klupp,L B Schwartz,D Printz,A Böhm,G Fritsch,H-P Horny,P Valent

The Journal of allergy and clinical immunology 117:626-34 PubMed16522463

2006

Identification of basophils by a mAb directed against pro-major basic protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas A Plager,Ellen A Weiss,Gail M Kephart,Robert M Mocharla,Ryoji Matsumoto,James L Checkel,Lawrence B Schwartz,Gerald J Gleich,Kristin M Leiferman

The Journal of allergy and clinical immunology 101:354-62 PubMed9525452

1998

Immunohistochemical detection of human basophils in late-phase skin reactions.

Applications

Unspecified application

Species

Unspecified reactive species

A M Irani,C Huang,H Z Xia,C Kepley,A Nafie,E D Fouda,S Craig,B Zweiman,L B Schwartz
View all publications

Product promise

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