AB110426

Anti-Bassoon/BSN antibody

Name: Anti-Bassoon/BSN antibody (ab110426) | Abcam
Brand: Abcam Limited
SKU: AB110426

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(2 Publications)

Rabbit Polyclonal Bassoon/BSN antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Rat, Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Protein within Human BSN aa 750-1050.

View Alternative Names

KIAA0434, ZNF231, BSN, Protein bassoon, Zinc finger protein 231

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody (AB110426)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody (AB110426)

Immunofluorescence of Neuromuscular junction whole muscle samples from adult mouse, stained with ab110426 at 1/400 dilution. Secondary (Alexa 488 goat antimouse IgG, IgM (H+L). Note : bright staining of the NMJ very selectively and in some terminals, very brightly.

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Western blot - Anti-Bassoon/BSN antibody (AB110426)

All lanes:

Western blot - Anti-Bassoon/BSN antibody (ab110426) at 1/1000 dilution

Lane 1:

Rat brain lysate at 10 µg

Lane 2:

Mouse brain lysate at 10 µg

Predicted band size: 416 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IHC-Fr, WB

applications

Immunogen

Recombinant Protein within Human BSN aa 750-1050. The exact immunogen used to generate this antibody is proprietary information.

Q9UPA5

Specificity

No cross-reactivity to Piccolo. Multiple isoforms can be detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For large isoforms reduce dilution.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bassoon also known as BSN or Bassoon protein functions as a major component in the active zone of synapses participating in the organization and regulation of neurotransmitter release. The protein has a reported molecular mass of approximately 420 kDa. Expression of the Bassoon protein takes place prominently in neuronal tissues especially within the central nervous system. Its structure and size contribute to its role in maintaining the integrity of synaptic architecture.

Biological function summary

Bassoon plays an important role in the presynaptic active zone by facilitating efficient synaptic vesicle docking and priming. It acts as a scaffold protein within the presynaptic terminal forming part of a larger molecular complex that includes Piccolo and other cytomatrix proteins. These scaffolding arrangements help ensure precise neurotransmitter release affecting synaptic strength and plasticity. Its integration into these complexes highlights its significance in neuronal communication and functionality.

Pathways

Bassoon actively participates in neurotransmission pathways especially those related to synaptic vesicle cycle and neurotransmitter release. It collaborates with proteins like RIM and Munc13 in these processes stabilizing the apparatus required for synaptic vesicle fusion and exocytosis. Through these interactions Bassoon contributes to the regulation of neurotransmitter release and is essential for proper synaptic function.

Bassoon links to various neurological conditions prominently neurodegenerative disorders like Alzheimer's disease and bipolar disorder. Changes in Bassoon expression or function could disrupt synaptic integrity thereby contributing to disease pathology. Research has associated alterations in calcium channel complexes which involve Bassoon with these disorders. Understanding Bassoon's molecular interactions may provide insights into the development and progression of such neurological diseases highlighting its significance in biomedical research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed : 12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed : 19380881). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity). Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity). Inhibits the activity of the proportion of DAO enzyme that localizes to the presynaptic active zone, which may modulate synaptic transmission (By similarity).

See full target information BSN

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:8420 PubMed38110419

2023

Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD.

Applications

Unspecified application

Species

Unspecified reactive species

Zhefan Stephen Chen,Mingxi Ou,Stephanie Taylor,Ruxandra Dafinca,Shaohong Isaac Peng,Kevin Talbot,Ho Yin Edwin Chan

Nature medicine 24:438-449 PubMed29529016

2018

Dentate granule cell recruitment of feedforward inhibition governs engram maintenance and remote memory generalization.

Applications

Unspecified application

Species

Unspecified reactive species

Nannan Guo,Marta E Soden,Charlotte Herber,Michael TaeWoo Kim,Antoine Besnard,Paoyan Lin,Xiang Ma,Constance L Cepko,Larry S Zweifel,Amar Sahay

View all publications

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Product promise

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Anti-Bassoon/BSN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody (AB110426)

Western blot - Anti-Bassoon/BSN antibody (AB110426)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise