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AB306594

Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free

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Mouse Recombinant Monoclonal Bassoon/BSN antibody. Carrier free. Suitable for IHC-P, IHC-Fr, ICC/IF and reacts with Mouse, Rat samples.

View Alternative Names

Kiaa0434, Bsn, Protein bassoon

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a BSN expression vector containing a his tag. (B) HEK-293T cells transfected with empty vector containing a his tag stained for Bassoon/BSN using ab306593 at a 1/20000 dilution, followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T cells transfected with a BSM expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab306593 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Bassoon/BSN with ab306593 at 1/5000 dilution (0.205 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on mouse liver. The section was incubated with ab306593 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Bassoon/BSN with ab306593 at 1/5000 dilution (0.205 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on rat cerebrum. The section was incubated with ab306593 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Bassoon/BSN with ab306593 at 1/5000 dilution (0.205 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab306593 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Frozen sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus (fresh) tissue labeling Bassoon/BSN with ab306593 at 1/50 dilution (20.46 µg/mL) followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on rat hippocampus. The nuclear counterstain was DAPI (Blue). The section was incubated with ab306593 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

Immunohistochemistry (Frozen sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Bassoon/BSN with ab306593 at 1/50 dilution (20.46 µg/mL) followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control : no staining on rat liver (PMID : 9679147). The nuclear counterstain was DAPI (Blue). The section was incubated with ab306593 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Bassoon/BSN with ab306593 at 1/5000 dilution (0.205 µg/ml) followed by ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Negative control : no staining on rat liver. The section was incubated with ab306593 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunocytochemistry/ Immunofluorescence - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bassoon/BSN antibody [L124-59] - BSA and Azide Free (AB306594)

This data was developed using ab306593, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling Bassoon/BSN with ab306593 at 1/50 dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/ml) (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (1 µg/ml) followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150088) at a 1/500 dilution (2 µg/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab306593 at a 1/50 dilution followed by ab150088 at a 1/500 dilution. -ve control 2 : ab183830 at a 1/1000 dilution followed by ab150113 at a 1/1000 dilution.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

L124-59

Isotype

IgG2b

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-Fr, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab306594 is a carrier free version of ab306593.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bassoon also known as BSN or Bassoon protein functions as a major component in the active zone of synapses participating in the organization and regulation of neurotransmitter release. The protein has a reported molecular mass of approximately 420 kDa. Expression of the Bassoon protein takes place prominently in neuronal tissues especially within the central nervous system. Its structure and size contribute to its role in maintaining the integrity of synaptic architecture.
Biological function summary

Bassoon plays an important role in the presynaptic active zone by facilitating efficient synaptic vesicle docking and priming. It acts as a scaffold protein within the presynaptic terminal forming part of a larger molecular complex that includes Piccolo and other cytomatrix proteins. These scaffolding arrangements help ensure precise neurotransmitter release affecting synaptic strength and plasticity. Its integration into these complexes highlights its significance in neuronal communication and functionality.

Pathways

Bassoon actively participates in neurotransmission pathways especially those related to synaptic vesicle cycle and neurotransmitter release. It collaborates with proteins like RIM and Munc13 in these processes stabilizing the apparatus required for synaptic vesicle fusion and exocytosis. Through these interactions Bassoon contributes to the regulation of neurotransmitter release and is essential for proper synaptic function.

Bassoon links to various neurological conditions prominently neurodegenerative disorders like Alzheimer's disease and bipolar disorder. Changes in Bassoon expression or function could disrupt synaptic integrity thereby contributing to disease pathology. Research has associated alterations in calcium channel complexes which involve Bassoon with these disorders. Understanding Bassoon's molecular interactions may provide insights into the development and progression of such neurological diseases highlighting its significance in biomedical research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed : 12628168, PubMed : 12628169, PubMed : 19812333). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (By similarity). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity) (PubMed : 28231469). Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity). Inhibits the activity of the proportion of DAO enzyme that localizes to the presynaptic active zone, which may modulate synaptic transmission (By similarity).
See full target information Bsn
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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