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AB317329

Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166]

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Human Recombinant Monoclonal Bat SHC014-CoV RBD antibody. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Bat SARS-like coronavirus RsSHC014, Transfected cell line - Bat SARS-like coronavirus RsSHC014 samples.
2 Images
Indirect ELISA - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (AB317329)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (AB317329)

Indirect ELISA analysis of ab317329 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : bat SARS-like coronavirus RsSHC014 Spike glycoprotein,SARS-CoV-2 (2019-nCoV) Spike glycoprotein,MERS-CoV Spike glycoprotein,HCoV-HKU1 Spike glycoprotein.

Antigen concentration : 1000 ng/ml

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (AB317329)
  • Dot

Supplier Data

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (AB317329)

Dot blot analysis of bat SARS-like coronavirus RsSHC014 Spike glycoprotein using ab317329 at 1 : 1000 followed by a Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (ab317329) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag whole cell lysate

Lane 2:

293T cells transfected with a Bat Spike glycoprotein expression vector containing a myc-his-tag whole cell lysate

Lane 3:

His-tagged bat SARS-like coronavirus RsSHC01 recombinant fragment protein

Secondary

All lanes:

Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

false

Exposure time: 180s

  • Carrier free

    Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] - BSA and Azide free

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

abd166

Isotype

IgG1

Carrier free

No

Reacts with

Bat SARS-like coronavirus RsSHC014

Applications

Dot, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with SARS-CoV-2 Spike glycoprotein, MERS-CoV Spike glycoprotein and HCoV-HKU1 Spike glycoprotein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bat SARS-like coronavirus RsSHC014": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Bat SARS-like coronavirus RsSHC014": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Bat SARS-like coronavirus RsSHC014": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Bat SHC014-CoV RBD
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com