AB236876

Anti-BATF antibody [EPR21911-55]

RabMAb
Recombinant
20ul selling size
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(6 Publications)

Rabbit Recombinant Monoclonal BATF antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 6 publications.

View Alternative Names

Basic leucine zipper transcriptional factor ATF-like, B-cell-activating transcription factor, SF-HT-activated gene 2 protein, B-ATF, SFA-2, BATF

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911-55] (AB236876)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911-55] (AB236876)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labeling BATF with ab236876 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear staining in KARPAS-299 cell line is observed.

Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at a 1/200 dilution (red). The nuclear counter stain is DAPI (blue). The control is KARPAS-299 cell line stained incubated with secondary antibody only (ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary).

Negative control : HeLa cell line（PMID : 8570175).

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911-55] (AB236876)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911-55] (AB236876)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line (left) and KARPAS-299 (human anaplastic large cell lymphoma) cell line (right)labeling BATF with ab236876 at 1/500 dilution (red) compared with a Rabbit monoclonal IgG - Isotype control (ab172730) (black) and an unlabeled control (cellsincubated withsecondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Negative control : HeLa (PMID : 8570175).

Unknown

Immunoprecipitation - Anti-BATF antibody [EPR21911-55] (AB236876)

BATF was immunoprecipitated from 0.35 mg KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab236876 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236876 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 μg (Input).
Lane 2 : ab236876 IP in KARPAS-299 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab236876 in KARPAS-299 whole cell lysate (-).

Blocking/Dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

We used a high sensitivity ECL substrate to develop this blot.

All lanes:

Immunoprecipitation - Anti-BATF antibody [EPR21911-55] (ab236876)

Predicted band size: 14 kDa

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Western blot - Anti-BATF antibody [EPR21911-55] (AB236876)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa (PMID : 8570175).

All lanes:

Western blot - Anti-BATF antibody [EPR21911-55] (ab236876) at 1/1000 dilution

Lane 1:

KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

A20 (Mouse reticulum sarcoma B lymphocyte cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 14 kDa

false

Exposure time: 3min

Carrier free

Anti-BATF antibody [EPR21911-55] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21911-55

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system : specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters : regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs) : up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs (By similarity).

See full target information BATF

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:826 PubMed40392374

2025

Visfatin promotes multiple myeloma cell proliferation and inhibits apoptosis by inducing IL-6 production via NF-κB pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Tie,Tao Ma,Jia Liu,Zhigang Yi,Hao Xiong,Jun Bai,Yanhong Li,Lijuan Li,Liansheng Zhang

Journal of translational medicine 22:558 PubMed38862971

2024

BATF promotes tumor progression and association with FDG PET-derived parameters in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Lu,Jun Liu,Lijuan Feng,Yan Huang,Yanfeng Xu,Cuicui Li,Wei Wang,Yin Kan,Jigang Yang,Mingyu Zhang

Immunity 56:1468-1484.e7 PubMed37285842

2023

Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ana C F Ferreira,Aydan C H Szeto,Paula A Clark,Alastair Crisp,Patrycja Kozik,Helen E Jolin,Andrew N J McKenzie

Aging 13:16009-16023 PubMed34096887

2021

Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxia Zhang,Min Lin,Junli Wang,Fenglian Yang,Peikui Yang,Yaqun Liu,Zikai Chen,Yuzhong Zheng

Annals of the New York Academy of Sciences 1474:61-72 PubMed32483833

2020

LncRNA-ES3 inhibition by Bhlhe40 is involved in high glucose-induced calcification/senescence of vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Yu Zhong,Xing-Jun Cui,Jun-Kun Zhan,Yan-Jiao Wang,Shuang Li,Xiao Lin,Qun-Yan Xiang,Yu-Qing Ni,Le Liu,You-Shuo Liu

Oncology letters 19:205-210 PubMed31897131

2020

BATF acts as an oncogene in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Feng,Liangbin Pan,Biao Zhang,Haitao Huang,Haitao Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BATF antibody [EPR21911-55]

Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911-55] (AB236876)

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911-55] (AB236876)

Immunoprecipitation - Anti-BATF antibody [EPR21911-55] (AB236876)

Western blot - Anti-BATF antibody [EPR21911-55] (AB236876)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Product promise