AB221146

Anti-BATF antibody [EPR21911]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal BATF antibody. Suitable for ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

Basic leucine zipper transcriptional factor ATF-like, B-cell-activating transcription factor, SF-HT-activated gene 2 protein, B-ATF, SFA-2, BATF

3 Images

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911] (AB221146)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911] (AB221146)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized KARPAS-299 (human anaplastic large cell lymphoma, right panel) or HeLa (human epithelial cell line from cervix adenocarcinoma, left panel) cell line labeling BATF with ab221146 at 1/500 (red) compared with a Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) (ab150077), at 1/2,000 dilution was used as the secondary antibody.

Negative control : HeLa cell line (PMID : 8570175).

Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911] (AB221146)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911] (AB221146)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labeling BATF with ab221146 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1,000 dilution (green). Confocal image showing nuclear staining in KARPAS-299 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution.

PBS only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1,000 dilution.

Negative control : HeLa (human epithelial cell line from cervix adenocarcinoma) (PMID : 8570175).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911] (AB221146)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A20 (mouse reticulum sarcoma) cell line labeling BATF with ab221146 at 1/500 (red) compared with a Isotype control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2,000 dilution was used as the secondary antibody.

Carrier free

Anti-BATF antibody [EPR21911] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21911

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

AP-1 family transcription factor that controls the differentiation of lineage-specific cells in the immune system : specifically mediates the differentiation of T-helper 17 cells (Th17), follicular T-helper cells (TfH), CD8(+) dendritic cells and class-switch recombination (CSR) in B-cells. Acts via the formation of a heterodimer with JUNB that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3'. The BATF-JUNB heterodimer also forms a complex with IRF4 (or IRF8) in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 (or IRF8) and activation of genes. Controls differentiation of T-helper cells producing interleukin-17 (Th17 cells) by binding to Th17-associated gene promoters : regulates expression of the transcription factor RORC itself and RORC target genes such as IL17 (IL17A or IL17B). Also involved in differentiation of follicular T-helper cells (TfH) by directing expression of BCL6 and MAF. In B-cells, involved in class-switch recombination (CSR) by controlling the expression of both AICDA and of germline transcripts of the intervening heavy-chain region and constant heavy-chain region (I(H)-C(H)). Following infection, can participate in CD8(+) dendritic cell differentiation via interaction with IRF4 and IRF8 to mediate cooperative gene activation. Regulates effector CD8(+) T-cell differentiation by regulating expression of SIRT1. Following DNA damage, part of a differentiation checkpoint that limits self-renewal of hematopoietic stem cells (HSCs) : up-regulated by STAT3, leading to differentiation of HSCs, thereby restricting self-renewal of HSCs (By similarity).

See full target information BATF

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112800 PubMed40585371

2025

The transcription factor IRF4 regulates the homeostasis and function of intestinal ILC3s.

Applications

Unspecified application

Species

Unspecified reactive species

Xianzhi Gao,Xin Shen,Qianying Xu,Yan Zeng,Linjia Dong,Shenghui Hong,Huihui Jin,Qianqian Wang,Di Wang,Linrong Lu,Lie Wang

The Journal of comparative neurology 532:e70003 PubMed39656141

2024

Developmental and Adult Striatal Patterning of Nociceptin Ligand Marks Striosomal Population With Direct Dopamine Projections.

Applications

Unspecified application

Species

Unspecified reactive species

Emily Hueske,Carrie Stine,Tomoko Yoshida,Jill R Crittenden,Akshay Gupta,Joseph C Johnson,Ananya S Achanta,Smitha Bhagavatula,Johnny Loftus,Ara Mahar,Dan Hu,Jesus Azocar,Ryan J Gray,Michael R Bruchas,Ann M Graybiel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BATF antibody [EPR21911]

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911] (AB221146)

Immunocytochemistry/ Immunofluorescence - Anti-BATF antibody [EPR21911] (AB221146)

Flow Cytometry (Intracellular) - Anti-BATF antibody [EPR21911] (AB221146)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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