Anti-BATF3 antibody [EPR26558-38]

7 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling BATF3 with ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human tonsil (PMID: 28878352). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Immunohistochemical analysis of paraffin-embedded Human anaplastic lar tissue labeling BATF3 with ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human anaplastic large cell lymphoma (PMID: 34202976). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling BATF3 with ab302568 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or abxxxxxx.

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  Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Ramos (human Burkitt's lymphoma B lymphocyte, Left) / KARPAS-299 (human anaplastic large cell lymphoma, Right) cells labelling BATF3 with ab302568 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: Ramos (PMID: 34202976).

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  Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labelling BATF3 with ab302568 at 1/500 (0.942 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in KARPAS-299 cell line.Negative control: Ramos (PMID: 34202976) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Immunohistochemical analysis of paraffin-embedded Human Hodgkin lympho tissue labeling BATF3 with ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human Hodgkin lymphoma (PMID: 34202976, PMID:28878352). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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  Western blot - Anti-BATF3 antibody [EPR26558-38] (ab302568)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Negative control: Ramos (PMID:34202976).

  All lanes: Western blot - Anti-BATF3 antibody [EPR26558-38] (ab302568) at 1/1000 dilution

  Lane 1: KARPAS-299 (human T cell lymphoma cell) whole cell lysate 20 µg

  Lane 2: HDLM-2 (human hodgkin lymphoma) whole cell lysate 20 µg

  Lane 3: SR (human pleural effusion lymphoblast) whole cell lysate 20 µg

  Lane 4: Ramos (human burkitts lymphoma b lymphocyte) whole cell lysate 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Observed band size: 18 kDa

  Exposure time: 81s