Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human anaplastic lar tissue labeling BATF3 with ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human anaplastic large cell lymphoma (PMID : 34202976). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human Hodgkin lympho tissue labeling BATF3 with ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human Hodgkin lymphoma (PMID : 34202976, PMID : 28878352). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling BATF3 with ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human tonsil (PMID : 28878352). The section was incubated with ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling BATF3 with ab302568 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab302568.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labelling BATF3 with ab302568 at 1/500 (0.942 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in KARPAS-299 cell line.Negative control : Ramos (PMID : 34202976) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation. Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Ramos (human Burkitt's lymphoma B lymphocyte, Left) / KARPAS-299 (human anaplastic large cell lymphoma, Right) cells labelling BATF3 with ab302568 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : Ramos (PMID : 34202976).

• WB

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Western blot - Anti-BATF3 antibody [EPR26558-38] - BSA and Azide free (AB302569)

This data was developed using ab302568, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : Ramos (PMID : 34202976).

All lanes:

Western blot - Anti-BATF3 antibody [EPR26558-38] (<a href='/en-us/products/primary-antibodies/batf3-antibody-epr26558-38-ab302568'>ab302568</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate 20 µg

Lane 2:

HDLM-2 (human hodgkin lymphoma) whole cell lysate 20 µg

Lane 3:

SR (human pleural effusion lymphoblast) whole cell lysate 20 µg

Lane 4:

Ramos (human burkitts lymphoma b lymphocyte) whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Observed band size: 18 kDa

false

Exposure time: 81s