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Rabbit Recombinant Monoclonal BATF3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (AB302569), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (AB302569), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (AB302569), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (AB302569), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (AB302569), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PFlow Cyt (Intra)IPICC/IF
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

AP-1 family transcription factor that controls the differentiation of CD8(+) thymic conventional dendritic cells in the immune system. Required for development of CD8-alpha(+) classical dendritic cells (cDCs) and related CD103(+) dendritic cells that cross-present antigens to CD8 T-cells and produce interleukin-12 (IL12) in response to pathogens (By similarity). Acts via the formation of a heterodimer with JUN family proteins that recognizes and binds DNA sequence 5'-TGA[CG]TCA-3' and regulates expression of target genes.

Alternative names

Basic leucine zipper transcriptional factor ATF-like 3, B-ATF-3, 21 kDa small nuclear factor isolated from T-cells, Jun dimerization protein p21SNFT, BATF3, SNFT

Recommended products

Rabbit Recombinant Monoclonal BATF3 antibody. Carrier free. Suitable for WB, IHC-P, Flow Cyt (Intra), IP, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR26558-38

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling BATF3 with Anti-BATF3 antibody [EPR26558-38] ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human tonsil (PMID: 28878352). The section was incubated with Anti-BATF3 antibody [EPR26558-38] ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human anaplastic lar tissue labeling BATF3 with Anti-BATF3 antibody [EPR26558-38] ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human anaplastic large cell lymphoma (PMID: 34202976). The section was incubated with Anti-BATF3 antibody [EPR26558-38] ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling BATF3 with Anti-BATF3 antibody [EPR26558-38] ab302568 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD11c conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or abxxxxxx.

  • Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Ramos (human Burkitt's lymphoma B lymphocyte, Left) / KARPAS-299 (human anaplastic large cell lymphoma, Right) cells labelling BATF3 with Anti-BATF3 antibody [EPR26558-38] ab302568 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: Ramos (PMID: 34202976).

  • Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 (human anaplastic large cell lymphoma) cells labelling BATF3 with Anti-BATF3 antibody [EPR26558-38] ab302568 at 1/500 (0.942 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear staining in KARPAS-299 cell line.Negative control: Ramos (PMID: 34202976) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human Hodgkin lympho tissue labeling BATF3 with Anti-BATF3 antibody [EPR26558-38] ab302568 at 1/500 (0.942 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in human Hodgkin lymphoma (PMID: 34202976, PMID:28878352). The section was incubated with Anti-BATF3 antibody [EPR26558-38] ab302568 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569), expandable thumbnail

    Western blot - Anti-BATF3 antibody [EPR26558-38] (BSA and Azide free) (ab302569)

    This data was developed using Anti-BATF3 antibody [EPR26558-38] ab302568, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: Ramos (PMID:34202976).

    All lanes: Western blot - Anti-BATF3 antibody [EPR26558-38] (Anti-BATF3 antibody [EPR26558-38] ab302568) at 1/1000 dilution

    Lane 1: KARPAS-299 (human T cell lymphoma cell) whole cell lysate 20 µg

    Lane 2: HDLM-2 (human hodgkin lymphoma) whole cell lysate 20 µg

    Lane 3: SR (human pleural effusion lymphoblast) whole cell lysate 20 µg

    Lane 4: Ramos (human burkitts lymphoma b lymphocyte) whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Observed band size: 18 kDa

    Exposure time: 81s

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: Ramos (PMID:34202976).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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