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AB270742

Anti-Bax antibody [2D2]

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(12 Publications)

Mouse Recombinant Monoclonal BAX antibody. Suitable for IP, WB and reacts with Human samples. Cited in 12 publications.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

2 Images
Immunoprecipitation - Anti-Bax antibody [2D2] (AB270742)
  • IP

Lab

Immunoprecipitation - Anti-Bax antibody [2D2] (AB270742)

Bax was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate (2μg in 0.35mg lysates) with ab270742 at 15.9μg/ml. Western blot was performed from the immunoprecipitate using ab270742 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.
Lane 1 : HeLa whole cell lysate 10μg (Input).
Lane 2 : ab270742 IP in HeLa whole cell lysate.
Lane 3 : Mouse monoclonal IgG instead of ab270742 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 secs.

All lanes:

Immunoprecipitation - Anti-Bax antibody [2D2] (ab270742)

Predicted band size: 21 kDa

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Western blot - Anti-Bax antibody [2D2] (AB270742)
  • WB

Lab

Western blot - Anti-Bax antibody [2D2] (AB270742)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Bax antibody [2D2] (ab270742) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 10 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

Predicted band size: 21 kDa

false

Exposure time: 37s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D2

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "15.9 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.477 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

International journal of chronic obstructive pulmonary disease 20:551-564 PubMed40078929

2025

PGAM5 Modulates Macrophage Polarization, Aggravating Inflammation in COPD via the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zheng,Yujie Wang,Jia Li,Shaomao Zheng,Lipeng Zhang,Qiaoyu Li,Fayu Ling,Qiuli Nie,Qiong Feng,Jing Wang,Chengji Jin

Journal of ovarian research 18:13 PubMed39856707

2025

Targeting UGT2B15 and NR1H4 interaction: a novel therapeutic strategy for polycystic ovary syndrome using naftopidil enantiomers.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufen Zheng,Zikai Chen,Miao Liang,Liting Zhou,Miaoru Wang,Silin Zhang,Shuyun Zhang,Lei Ma,Wei Yi,Xiawen Liu

Molecular therapy. Oncology 32:200898 PubMed39640863

2024

Newcastle disease virus harboring the PTEN gene inhibits pancreatic cancer growth by inhibiting PI3K/AKT/mTOR signaling and activating apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Seonhee Kim,Bo-Kyoung Jung,Jinju Kim,Joo Hee Jeon,Sung Hoon Jang,Minsoo Kim,Cuk-Seong Kim,Hyun Jang

Discover. Oncology 14:28 PubMed36853387

2023

Estrogen-dependent activation of NCOA3 couples with p300 and NF-κB to mediate antiapoptotic genes in ER-positive breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Zhiyong Zhou

Journal of biochemical and molecular toxicology 36:e23118 PubMed35707935

2022

Negative regulation of miR-1288-3p/KRT4 axis through a circular RNA in oral cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Wang,Xin Ma,Jie Yu,Ying Lou

International heart journal 63:578-590 PubMed35650158

2022

LncRNA SNHG15 Modulates Ischemia-Reperfusion Injury in Human AC16 Cardiomyocytes Depending on the Regulation of the miR-335-3p/TLR4/NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haibo Du,Lianqin Ding,Tian Zeng,Di Li,Li Liu

Frontiers in medicine 8:658926 PubMed34458282

2021

Isoflurane Suppresses Proliferation, Migration, and Invasion and Facilitates Apoptosis in Colorectal Cancer Cells Through Targeting miR-216.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Cai,Liangyuan Suo,Zeqing Huang

Evidence-based complementary and alternative medicine : eCAM 2021:5523726 PubMed34422071

2021

Effect of Warm Acupuncture Combined with Bone Marrow Mesenchymal Stem Cells Transplantation on Cartilage Tissue in Rabbit Knee Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Wei Liu,Yong-Li Wu,Wei Wei,Yan-Ling Zhang,Di Liu,Xiao-Xiu Ma,Chun Li,Yu-Yuan Ma

Cancer cell international 21:412 PubMed34353342

2021

Blocking circ-SCMH1 (hsa_circ_0011946) suppresses acquired DDP resistance of oral squamous cell carcinoma (OSCC) cells both in vitro and in vivo by sponging miR-338-3p and regulating LIN28B.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Qiu,Bin Qiao,Nan Zhang,Zheng Fang,Lu Feng,Shanfeng Zhang,Weiliu Qiu

Frontiers in cell and developmental biology 9:688070 PubMed34386493

2021

Surface PEGylated Cancer Cell Membrane-Coated Nanoparticles for Codelivery of Curcumin and Doxorubicin for the Treatment of Multidrug Resistant Esophageal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Gao,Yue Zhu,Xiaopeng Xu,Fangjun Wang,Weidong Shen,Xia Leng,Jiyi Zhao,Bingtuan Liu,Yangyun Wang,Pengfei Liu
View all publications
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