AB3191

Anti-Bax antibody [5B7]

Recombinant
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(39 Publications)

Mouse Recombinant Monoclonal BAX antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 39 publications.

View Alternative Names

Apoptosis regulator BAX, Bax

3 Images

Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (AB3191)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (AB3191)

Intracellular flow cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at a 1.043μg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

Supplier Data

Immunoprecipitation - Anti-Bax antibody [5B7] (AB3191)

Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9μg/ml. Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
Lane 1 : RAW 264.7 whole cell lysate 10ug (Input).
Lane 2 : ab3191 IP in RAW 264.7 whole cell lysate.
Lane 3 : Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)

Predicted band size: 21 kDa

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Supplier Data

Western blot - Anti-Bax antibody [5B7] (AB3191)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Bax antibody [5B7] (ab3191) at 0.417 mg/mL

Lane 2:

Western blot - Anti-Bax antibody [5B7] (ab3191) at 0.417 µg/mL

Lane 1:

A20 (Mouse reticulum sarcoma B lymphocyte) lysate at 20 µg

Lane 2:

C2C12 (Mouse myoblasts) lysate at 20 µg

Predicted band size: 21 kDa

false

Exposure time: 26s

Carrier free

Anti-Bax antibody [5B7] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B7

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Antibody raised against a synthetic peptide that is not shared by rat or human Bax.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "13.9 µg/mL", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.417 µg/mL", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1.043 µg/mL", "FlowCytIntra-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.

Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. BAX deficiency leads to lymphoid hyperplasia and male sterility, because of the cessation of sperm production.

See full target information Bax

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40862741

2025

Chromatin Remodeler RSF1 as an Oncogenic Driver and Therapeutic Target in Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Du,Zhili Jia,Yao Lin,Xudong Zhao,Gengsheng Cao,Hengbin Wang

Theranostics 15:7653-7676 PubMed40756350

2025

Multi-dimensional data-driven computational drug repurposing strategy for screening novel neuroprotective agents in ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Qingqi Meng,Qing Liu,Yan Mi,Libin Xu,Feng Wang,Danyang Mu,Yueyang Liu,Yuxin Yang,Yongye Huang,Dakuo He,Yue Hou

Histology and histopathology :18958 PubMed40654025

2025

SLMO2 inhibits apoptosis in ovarian cancer cells by modulating mitochondrial function via TRIAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Wang,Yuesong Wang,Zixuan Li,Tianmei Zhang

NPJ Parkinson's disease 11:55 PubMed40122927

2025

Voluntary exercise alleviates neural functional deficits in Parkinson's disease mice by inhibiting microglial ferroptosis via SLC7A11/ALOX12 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinghui Xu,Xiaofei He,Lili Li,Liying Zhang,Mingyue Li,Yating Mu,Xiaofeng Yang,Shiyin Li,Yifeng Feng,Zejie Zuo,Yunqi Xu,Xiquan Hu,Haiqing Zheng

Veterinary sciences 12: PubMed39852882

2025

Anti-Stemness and Anti-Proliferative Effects of Cadmium on Bovine Mammary Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Penggang Liu,Xueli Chen,Yuqing Zhao,Waseem Ali,Tianle Xu,Jing Sun,Zongping Liu

iScience 27:111487 PubMed39759007

2025

miR-450b-5p promotes development of endometriosis by inhibiting the GABPA/HOXD10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Huang,Yidan Wang,Ruiyun Li,Yongmei Liu,Yuan Yang

Nature communications 15:7499 PubMed39209877

2024

A singular plasmonic-thermoelectric hollow nanostructure inducing apoptosis and cuproptosis for catalytic cancer therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yang,Zhiyu Zhao,Boshi Tian,Meiqi Yang,Yushan Dong,Bingchen Zhou,Shili Gai,Ying Xie,Jun Lin

Experimental and therapeutic medicine 27:213 PubMed38590560

2024

TRIM21 silencing inhibits the apoptosis and expedites the osteogenic differentiation of dexamethasone‑induced MC3T3‑E1 cells by activating the Keap1/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Shi,Li Chen,Xu Wang,Xin Ma

Cellular signalling 117:111087 PubMed38316266

2024

FATP2 activates PI3K/Akt/mTOR pathway by inhibiting ATF3 and promotes the occurrence and development of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Jiang,Ru Chen,Bing Hu,Situ Xiong,Sheng Li,Bin Fu,Xiaoqiang Liu

Journal of cellular and molecular medicine 27:3911-3927 PubMed37749949

2023

Apocynin exerts cytoprotective effects on dexamethasone-induced osteoblasts by inhibiting oxidative stress through the Nrf2 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglong Zhang,Ran Pang,Kai Zhang,Qian Xu,Chunlei Xu,Wei Shi,Xinyu Liang,Dong Li,Wenhao Cui,Shucai Bai,Zhijun Li,Hui Li,Huafeng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Bax antibody [5B7]

Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (AB3191)

Immunoprecipitation - Anti-Bax antibody [5B7] (AB3191)

Western blot - Anti-Bax antibody [5B7] (AB3191)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (39)

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Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise