JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB252258

Anti-Bax antibody [5B7] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Recombinant Monoclonal BAX antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse samples.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

3 Images
Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

Intracellular Flow Cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at 1.043μg/ml (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)
  • IP

Supplier Data

Immunoprecipitation - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9μg/ml . Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
Lane 1 : RAW 264.7 whole cell lysate 10ug (Input).
Lane 2 : ab3191 IP in RAW 264.7 whole cell lysate.
Lane 3 : Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Bax antibody [5B7] (<a href='/en-us/products/primary-antibodies/bax-antibody-5b7-ab3191'>ab3191</a>)

Predicted band size: 21 kDa

false

Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)
  • WB

Supplier Data

Western blot - Anti-Bax antibody [5B7] - BSA and Azide free (AB252258)

This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab3191).

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Bax antibody [5B7] (<a href='/en-us/products/primary-antibodies/bax-antibody-5b7-ab3191'>ab3191</a>) at 0.417 mg/mL

Lane 2:

Western blot - Anti-Bax antibody [5B7] (<a href='/en-us/products/primary-antibodies/bax-antibody-5b7-ab3191'>ab3191</a>) at 0.417 µg/mL

Lane 1:

A20 (Mouse reticulum sarcoma B lymphocyte) lysate at 20 µg

Lane 2:

C2C12 (Mouse myoblasts) lysate at 20 µg

Predicted band size: 21 kDa

false

Exposure time: 26s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5B7

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Mouse

Applications

IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Antibody raised against a synthetic peptide that is not shared by rat or human Bax.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "13.9 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.417 µg/mL", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1.043 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab252258 is the carrier-free version of ab3191.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB262929

Anti-Bax antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB262929)

  • 0
  • 0
Primary Antibodies

AB81083

Anti-Bax antibody [SP47]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] (AB81083)

  • 0
  • 0
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB216985

Anti-Bax antibody [E63] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] - BSA and Azide free (AB216985)

  • 0
  • 0
Primary Antibodies

AB240930

Anti-Bax antibody [SP47] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [SP47] - BSA and Azide free (AB240930)

  • 0
  • 0
Primary Antibodies

AB182733

Anti-Bax antibody [EPR18283]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunoprecipitation - Anti-Bax antibody [EPR18283] (AB182733)

  • 4
  • 6

Complementary Products

Assay Kits

AB199080

Human Bax ELISA Kit

Recombinant|SimpleStep

ELISA - Human Bax ELISA Kit (AB199080)

  • 0
  • 0
Primary Antibodies

AB13847

Anti-Caspase-3 antibody

KO Validated

Western blot - Anti-Caspase-3 antibody (AB13847)

  • 4
  • 56
Primary Antibodies

AB182858

Anti-Bcl-2 antibody [EPR17509]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-2 antibody [EPR17509] (AB182858)

  • 5
  • 13
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com