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AB104156

Anti-Bax antibody

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(22 Publications)

Rabbit Polyclonal BAX antibody. Suitable for WB and reacts with Human samples. Cited in 22 publications.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

3 Images
Western blot - Anti-Bax antibody (AB104156)
  • WB

Unknown

Western blot - Anti-Bax antibody (AB104156)

All lanes:

Western blot - Anti-Bax antibody (ab104156) at 1 µg/mL

All lanes:

Human lymph node tissue lysate - total protein (<a href='/en-us/products/unavailable/human-lymph-node-tissue-lysate-total-protein-ab29871'>ab29871</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Exposure time: 4min

Western blot - Anti-Bax antibody (AB104156)
  • WB

Lab

Western blot - Anti-Bax antibody (AB104156)

Lanes 1, 3 and 5 : Wild-type HAP1 cell lysate (20 μg)
Lanes 2, 4 and 6 : Bax knockout HAP1 cell lysate (20 μg)
Lanes 1 and 2 : Green signal from target – ab104156 observed at 20 kDa
Lanes 3 and 4 : Red signal from loading control – ab8226 observed at 42 kDa
Lanes 5 and 6 : Merged (red and green) signal

ab104156 was shown to react with Bax when Bax knockout samples were used, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab104156 and ab8226 (loading control to beta actin) were diluted 1 μg/mL and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Bax antibody (ab104156)

Predicted band size: 21 kDa

false

Western blot - Anti-Bax antibody (AB104156)
  • WB

Lab

Western blot - Anti-Bax antibody (AB104156)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Bax knockout HAP1 cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab104156 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa or ab18058, observed at 130 kDa.

This western blot image is a comparison between ab104156 and a competitor's top cited rabbit polyclonal antibody.

All lanes:

Western blot - Anti-Bax antibody (ab104156)

Predicted band size: 18 kDa,21 kDa,319 kDa,37 kDa,39 kDa,41 kDa,46 kDa,55 kDa,60 kDa,65 kDa,68 kDa,78 kDa,95 kDa

Observed band size: 66 kDa,78 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 37:e23410 PubMed37393518

2023

Circ_0005615 promotes cervical cancer cell growth and metastasis by modulating the miR-138-5p/KDM2A axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoning Luo,Jiewen Liu,Xiangcai Wang,Yili Wang,Jun Yuan,Yu Zhang

Bioengineering (Basel, Switzerland) 9: PubMed36354566

2022

Characteristics and Resistance to Cisplatin of Human Neuroblastoma Cells Co-Cultivated with Immune and Stromal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina V Kitaeva,Daria S Chulpanova,Margarita N Zhuravleva,Ivan Yu Filin,Ruslan M Deviatiiarov,Alyssa C Ballard-Reisch,Albert A Rizvanov,Valeriya V Solovyeva

Environmental toxicology 37:2855-2864 PubMed36052886

2022

Downregulation of circ-ZNF644 alleviates LPS-induced HK2 cell injury via miR-335-5p/HIPK1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Junzuo Gong,Shiqiao Zhao,Shu Luo,Songlin Yin,Xiaofeng Li,Yao Feng

Chinese medicine 17:102 PubMed36042482

2022

Traditional Chinese Medicine Fufang-Zhenzhu-Tiaozhi capsule prevents renal injury in diabetic minipigs with coronary heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Song,Ke Wang,Jianying Yin,Yiqi Yang,Bo Li,Dongxing Zhang,Hong Wang,Weixuan Wang,Wenjing Zhan,Caijuan Guo,Zhanhui Gu,Lexun Wang,Zhihuan Zeng,Weijian Bei,Xianglu Rong,Jiao Guo

The European journal of neuroscience 56:4304-4316 PubMed35766911

2022

Circ_TLK1 knockdown alleviates oxygen-glucose deprivation/reoxygenation-induced PC12 cell injury by regulating microRNA-136-5p/follistatin like-1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenduo Zhang,Jinbo He,Baoliang Wang

Acta biochimica et biophysica Sinica 54:524-536 PubMed35607959

2022

RETREG1-mediated ER-phagy activation induced by glucose deprivation alleviates nucleus pulposus cell damage via ER stress pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjin Luo,Huaizhen Liang,Weifeng Zhang,Gaocai Li,Kangcheng Zhao,Wenbin Hua,Yu Song,Cao Yang

Thoracic cancer 13:934-946 PubMed35307984

2022

CircATIC inhibits esophageal carcinoma progression and promotes radiosensitivity by elevating RHCG through sponging miR-10-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Ruitai Fan,Deyao Zhao,Peipei Liu,Zheng Yang,Junqi Liu,Song Zhang,Shilei Rao,Yang Wang,Lixin Wan

Frontiers in cardiovascular medicine 8:731890 PubMed34912856

2021

Hsa_circ_0003204 Knockdown Weakens Ox-LDL-Induced Cell Injury by Regulating miR-188-3p/TRPC6 Axis in Human Carotid Artery Endothelial Cells and THP-1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjia Peng,Shuai Li,Shiyue Chen,Jiacheng Yang,Ze Sun

Histology and histopathology 37:137-149 PubMed34825699

2021

Circ-SFMBT2 facilitates the malignant growth of acute myeloid leukemia cells by modulating miR-582-3p/ZBTB20 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Chang,Zhen Shang,Xi Ming,Jiaying Wu,Yi Xiao

Investigative ophthalmology & visual science 62:25 PubMed34269814

2021

Salidroside Prevents Hypoxia-Induced Human Retinal Microvascular Endothelial Cell Damage Via miR-138/ROBO4 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Shi,Nuo Dong,Qi Qiu,Shanhua Li,Jiaxing Zhang
View all publications
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