Mouse Polyclonal BAX antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human BAX aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
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- Chemistry & biodiversity 20:e2023012282023Bioevaluation of Spiro N-Propargylic β-Enaminones as Anti-Breast Cancer Agents: In Vitro and Molecular Docking Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHarika Atmaca,Suleyman Ilhan,Buse Aysen Dundar,Metin ZoraPubMed 37837366
- Cell death & disease 14:6012023Minnelide combined with anti-ANGPTL3-FLD monoclonal antibody completely protects mice with adriamycin nephropathy by promoting autophagy and inhibiting apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaowei Ji,Junchao Liu,Ye Yin,Hong Xu,Qian Shen,Jian YuPubMed 37689694
- Nutrients 15:2023Comparison of the Effects of Monounsaturated Fatty Acids and Polyunsaturated Fatty Acids on Liver Lipid Disorders in Obese Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Liu,Min Zhu,Meng Gong,Wen Zheng,Xin Zeng,Qing Zheng,Xiaoyu Li,Fudong Fu,Yingyi Chen,Jingqiu Cheng,Zhiyong Rao,Yanrong Lu,Younan ChenPubMed 37513618
- Acta biochimica Polonica 70:271-2762023Overexpression of long non-coding RNA GASL1 induces apoptosis and G0/G1 cell cycle arrest in human oral cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zhang,Wanjun Tao,Lei YuPubMed 37307139
- Journal of traditional and complementary medicine 13:500-5102023L. extracts ameliorate doxorubicin-induced acute kidney injury via modulation of oxidative stress, inflammation, and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSachinthi S Amarasiri,Anoja P Attanayake,Liyanage D A M Arawwawala,Lakmini K B Mudduwa,Kamani A P W JayatilakaPubMed 37693098
- Scientific reports 13:21112023Exogenous melatonin alleviates neuropathic pain-induced affective disorders by suppressing NF-κB/ NLRP3 pathway and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTahmineh Mokhtari,Lu-Peng Yue,Li HuPubMed 36747075
- Proteome science 21:12023Proteomic Analysis of Protective Effects of Dl-3-n-Butylphthalide against mpp + -Induced Toxicity via downregulating P53 pathway in N2A Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Zhao,Jian Zhang,Yidan Zhang,Shuyue Li,Ya Gao,Cui Chang,Xiang Liu,Lei Xu,Guofeng YangPubMed 36597095
- International journal of molecular sciences 23:2022Melatonin Type 2 Receptor Activation Regulates Blue Light Exposure-Induced Mouse Corneal Epithelial Damage by Modulating Impaired Autophagy and Apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRujun Jin,Ying Li,Hui Jin,Hee Su Yoon,Ji Suk Choi,Jonghwa Kim,Hyeon Jeong Yoon,Kyung Chul YoonPubMed 36232639
- Acta biochimica Polonica 69:417-4222022USP18 contributes to the proliferation and migration of ovarian cancer cells by regulating the AKT/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiqin Liu,Shile Gao,Xingjun Xu,Lijie Zhang,Bin Xu,Donghui LuPubMed 35696596
- Allergologia et immunopathologia 50:16-222022Dexmedetomidine represses TGF-β1-induced extracellular matrix production and proliferation of airway smooth muscle cells by inhibiting MAPK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRong Zhou,Xiaoyan ChenPubMed 35257541
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human BAX aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q07812
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:36361894, PubMed:8358790, PubMed:8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816).
Alternative names
BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
Recommended products
Mouse Polyclonal BAX antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human BAX aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human BAX aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q07812
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
- Biological function summary
The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.
- Pathways
Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.
- Associated diseases and disorders
The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (ab216494)
HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (ab216494) 1:100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei
Western blot - Anti-Bax antibody (ab216494)
All lanes: Western blot - Anti-Bax antibody (ab216494) at 1/300 dilution
All lanes: mouse cerebrum lysate
Predicted band size: 21 kDa
Observed band size: 21 kDa
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