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AB216494

Anti-Bax antibody

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(36 Publications)

Mouse Polyclonal BAX antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human BAX aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (AB216494)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (AB216494)

HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) polyclonal Antibody, Unconjugated (ab216494) 1 : 100, 90 minutes at 37°C; followed by a CY3 conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei

Western blot - Anti-Bax antibody (AB216494)
  • WB

Supplier Data

Western blot - Anti-Bax antibody (AB216494)

All lanes:

Western blot - Anti-Bax antibody (ab216494) at 1/300 dilution

All lanes:

mouse cerebrum lysate

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human BAX aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q07812

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
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Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:330 PubMed40886189

2025

Melatonin attenuates intervertebral disc degeneration by restoring mitochondrial homeostasis through PGC-1α signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Lan,Wenhan Yang,Bin Yan,Weizhuang Guo,Yuantao Zhang

Investigative ophthalmology & visual science 66:39 PubMed40105819

2025

Targeted siRNA Delivery Against RUNX1 Via tFNA: Inhibiting Retinal Neovascularization and Restoring Vessels Through Dll4/Notch1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodi Zhou,Xiaoxiao Xu,Qiong Wang,Yanting Lai,Linyan Zhang,Yunfeng Lin,Xiaoyan Ding,Limei Sun

Molecular medicine (Cambridge, Mass.) 30:272 PubMed39719558

2024

Doublecortin regulates the mitochondrial-dependent apoptosis in glioma via Rho-A/Net-1/p38-MAPK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Iqra Nadeem,Zhou Han,Hong Xiaoliang,Seyram Yao Adzraku,Piniel Alphayo Kambey,Kouminin Kanwore,Mu Peipei,Adebayo Oluwafemi Adekunle,Joseph Adu-Amankwaah,Abiola Abdulrahman Ayanlaja,Yi Zheng,Gao Dianshuai,Xiaomei Liu,Yuanjian Song

Cancer science 116:544-558 PubMed39618050

2024

LncRNA MIR600HG inhibits laryngeal cancer development by mediating the miR-424-5p/BTG2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowen Zhu,Min Zhong,Qingdong Wang,MeiJia Zhang

American journal of translational research 16:5516-5524 PubMed39544778

2024

Protection against cigarette smoke-induced chronic obstructive pulmonary disease via activation of the SIRT1/FoxO1 axis by targeting microRNA-132.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Shen,Jing Chen,Suzhen Yang,Hui Zhang,Hui Yu,Sha Wang,Jianmin Li

Cancer biology & therapy 25:2427374 PubMed39540618

2024

The tubulin polymerization inhibitor gambogenic acid induces myelodysplastic syndrome cell apoptosis through upregulation of Fas expression mediated by the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Zhong,Shijun Wang,Lei Xia,Xiaoman Yang,Liguang Fang,Xianyi Zhang,Mengyue Wang,Haijun Zhao,Guanghui Wang,Jinglong Wu,Ruijian Guo,Ming Zhong,Eiichi Gohda

Aging 16:12769-12780 PubMed39383058

2024

Bezafibrate mitigates cardiac injury against coronary microembolization by preventing activation of p38 MAPK/NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Liu,Wenfang Wang,Wenfeng Li

Pharmaceuticals (Basel, Switzerland) 17: PubMed39338354

2024

The Effect of Chronic Immunosuppressive Regimen Treatment on Apoptosis in the Heart of Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Surówka,Michał Żołnierczuk,Piotr Prowans,Marta Grabowska,Patrycja Kupnicka,Marta Markowska,Zbigniew Szlosser,Karolina Kędzierska-Kapuza

ACS omega 9:23713-23723 PubMed38854531

2024

Evaluation of Novel Spiro-pyrrolopyridazine Derivatives as Anticancer Compounds: In Vitro Selective Cytotoxicity, Induction of Apoptosis, EGFR Inhibitory Activity, and Molecular Docking Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Harika Atmaca,Suleyman Ilhan,Çisil Çamli Pulat,Buse Aysen Dundar,Metin Zora

Discover oncology 15:88 PubMed38536591

2024

Circ_0020460 drives tumorigenesis in cervical cancer through miR-485-3p sponging.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Yan,Chunyan Hu,Yali Cheng,Lingzhi Zheng,Baojin Zeng,Sujun Zhao,Chen Liu
View all publications
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