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AB53154

Anti-Bax antibody

1

(1 Review)

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(351 Publications)

Rabbit Polyclonal BAX antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 351 publications. Immunogen corresponding to Synthetic Peptide within Human BAX.

View Alternative Names

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB53154)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB53154)

ab53154 staining human normal gallbladder tissue. Staining is localized to cell membrane and cytoplasm.
Left panel : ab53154 at 4 μg/ml. Right panel : Isotype control.
Sections were stained using an automated system at room temperature. Sections were rehydrated and antigen retrieved with the EDTA pH 9.0. Slides were blocked in 3% H2O2, methanol for 10 minutes. They were then blocked for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB53154)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody (AB53154)

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using ab53154 at 1/50 dilution. The picture on the right is treated with the synthesized peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (AB53154)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bax antibody (AB53154)

Immunofluorescence analysis of A549 (Human lung carcinoma cell line) cells, using ab53154 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

Western blot - Anti-Bax antibody (AB53154)
  • WB

Unknown

Western blot - Anti-Bax antibody (AB53154)

All lanes:

Western blot - Anti-Bax antibody (ab53154) at 1/500 dilution

Lane 1:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts with immunizing peptide

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human BAX. The exact immunogen used to generate this antibody is proprietary information.

Q07812

Specificity

Preliminary customer data suggests that the current lot may not work in rat species, therefore it will be removed as a validated species until new testing is available.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).
See full target information BAX
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Publications (351)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1623153 PubMed41019996

2025

Dehydrozaluzanin C inhibits colon cancer cell proliferation, apoptosis and cycle arrest through peroxisome proliferator-activated receptor γ (PPARγ) activation.

Applications

Unspecified application

Species

Unspecified reactive species

Shan-Shan Li,Zhao-Ting Li,Xiao-Qing Zhu,Xu Li,Xi-Ke Xu,Xian-Peng Zu,Xian Li,Yun-Heng Shen

Journal of cell communication and signaling 19:e70044 PubMed40917114

2025

Trans-Coumaryl acetate mediates GRK5/NF-κB/Nrf2 signaling axis to ameliorate septic acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Yugang Deng,Kunyang Lei,Yaqi Li,Siwei Ma

Oncology research 33:1473-1484 PubMed40486873

2025

Gallic acid suppresses esophageal squamous cell carcinoma progression and enhances cisplatin chemosensitivity through IL-6/STAT3/Notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nuran Bedolla,Hao Wu,Linyu Liu,Xueting Liu,Yanli Ren

BMC pharmacology & toxicology 26:83 PubMed40229902

2025

Sevoflurane reduces cardiomyocyte injury in a hypoxia/reoxygenation model of cardiomyocytes through the linc01278/miR-134-5pt regulatory axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Wang,Changhua Zhu,Yangge Shao,Rui Chen,Hui Liang

Journal of nanobiotechnology 23:180 PubMed40050959

2025

Efficient delivery of anlotinib and radioiodine by long circulating nano-capsules for active enhanced suppression of anaplastic thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhang,Chuanying Zhu,Shuo Huang,Miaomiao Xu,Chao Li,Hongliang Fu,Yafu Yin,Sheng Liang,Hui Wang,Zhilei Cui,Lei Huang

Chinese medicine 20:11 PubMed39815349

2025

Tangeretin alleviates sepsis-induced acute lung injury by inhibiting ferroptosis of macrophage via Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zhang,Yan Wang,Shenghua Wang,Xiaomei Xue,Kai Huang,Dunfeng Xu,Lai Jiang,Siyuan Li,Yunqian Zhang

International journal of molecular sciences 26: PubMed39795860

2025

Adropin/Tirzepatide Combination Mitigates Cardiac Metabolic Aberrations in a Rat Model of Polycystic Ovarian Syndrome, Implicating the Role of the AKT/GSK3β/NF-κB/NLRP3 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Islam Ibrahim Hegab,Hemat El-Sayed El-Horany,Rania Nagi Abd-Ellatif,Nahla Anas Nasef,Asmaa H Okasha,Marwa Nagy Emam,Shereen Hassan,Walaa S Elseady,Doaa A Radwan,Rasha Osama ElEsawy,Yasser Mostafa Hafez,Maha Elsayed Hassan,Nouran Mostafa Mansour,Gamaleldien Elsayed Abdelkader,Mohamed H Fouda,Amira M Abd El Maged,Hanan M Abdallah

Scientific reports 14:28864 PubMed39572680

2024

The effects of hesperidin on valproic acid-induced reduction in hippocampal neurogenesis through the antioxidant and apoptotic pathways in adult rats.

Applications

Unspecified application

Species

Unspecified reactive species

Soraya Kaewngam,Ram Prajit,Tanaporn Anosri,Kornrawee Suwannakot,Rasa Saenno,Nataya Sritawan,Anusara Aranarochana,Apiwat Sirichoat,Wanassanun Pannangrong,Peter Wigmore,Jariya Umka Welbat

Biomolecules & biomedicine 25:165-176 PubMed39388706

2024

miR-149-3p targeting regulates the sensitivity to cisplatin to inhibit the progression of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Qin,Dongfang Tang,Mingzhi Zhang

Iranian journal of immunology : IJI 21:186-200 PubMed39319693

2024

JAK/STAT Signaling Pathway Mediates Anti-Tumor Immunity of CD8+ T Cells in Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Shao,Gang Deng,Guojun Wen,Xi Xie
View all publications
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