Anti-Bax antibody [E63] is a rabbit recombinant monoclonal antibody that is used to detect Bax in IHC-P, IP, Western blot, sELISA. Suitable for Human, Mouse, Rat samples.
- Cited in over 2475 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Validated on the Leica BOND™ RX automated IHC staining platform
- Specificity confirmed with BAX knockout cell line validation
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
IHC-P | ICC/IF | IP | Flow Cyt | WB | sELISA | |
---|---|---|---|---|---|---|
Human | Tested | Not recommended | Tested | Not recommended | Tested | Expected |
Mouse | Expected | Not recommended | Expected | Not recommended | Tested | Expected |
Rat | Tested | Not recommended | Expected | Not recommended | Tested | Expected |
Cow | Predicted | Not recommended | Predicted | Not recommended | Predicted | Predicted |
Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
Recombinant full length protein - Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Human, Cow, Recombinant full length protein - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info - | Notes - |
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
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Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:36361894, PubMed:8358790, PubMed:8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816).
BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4
Anti-Bax antibody [E63] is a rabbit recombinant monoclonal antibody that is used to detect Bax in IHC-P, IP, Western blot, sELISA. Suitable for Human, Mouse, Rat samples.
- Cited in over 2475 publications
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Validated on the Leica BOND™ RX automated IHC staining platform
- Specificity confirmed with BAX knockout cell line validation
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.
Anti-Bax antibody [E63] (ab32503) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, IP, WB, sELISA in human, mouse, rat samples.
Anti-Bax antibody [E63] (ab32503) has been cited over 2479 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-Bax antibody [E63] (ab32503) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-Bax antibody [E63] (ab32503) has been confirmed by testing in knockout samples.
Anti-Bax antibody [E63] (ab32503) has 12 independent reviews from customers.
Anti-Bax antibody [E63] (ab32503) specifically detects Bax (UniProt ID: Q07812; Molecular weight: 21kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone E63 - Anti-Bax antibody [E63] - BSA and Azide free ab216985.
Antibody clone E63 is also available pre-conjugated to a variety of labels for your convenience - HRP (HRP Anti-Bax antibody [E63] ab205822).
The BAX gene (BCL2 Associated X) is a key player in the regulation of apoptosis, or programmed cell death, which is crucial for maintaining cellular homeostasis and preventing cancer. BAX is part of the BCL-2 protein family and promotes apoptosis by increasing mitochondrial membrane permeability, leading to the release of cytochrome c and activation of caspases. In oncology, BAX expression is regulated by the tumor suppressor protein p53, which is often mutated in cancer. Mutations or dysregulation of BAX can contribute to cancer development and progression by allowing cancer cells to survive.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.
The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.
Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.
The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
Purified ab32503 staining Bax in Human lung carcinoma tissue section by immmunohistochemistry (IHC-P- Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraffin and heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0). Samples were incubated with primary antibody at 1:500 dilution. A goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody at 1:500 dilution. Cytoplasmic staining on human lung carcinoma.
Purified ab32503 immunoprecipitating Bax in HeLa lysates. For western blotting, the primary antibody used was purified ab32503 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection at 1/1000 dilution. Capture antibody was used at a 1/20 dilution. Blocking and diluting buffer used was 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Bax antibody [E63] (ab32503)
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1s
This western blot image is a comparison between ab32503 and a competitor's top cited rabbit polyclonal antibody.
All lanes: Western blot - Anti-Bax antibody [E63] (ab32503)
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: Bax knockout HAP1 cell lysate at 20 µg
Predicted band size: 21 kDa
All lanes: Western blot - Anti-Bax antibody [E63] (ab32503) at 1/1000 dilution
Lane 1: Wild-type HAP1 cell lysate at 20 µg
Lane 2: Bax knockout HAP1 cell lysate at 20 µg
Predicted band size: 21 kDa
ab32503 was shown to react with Bax in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BAX knockout HeLa cell line ab255363 (knockout cell lysate Human BAX knockout HeLa cell lysate ab263841) was used. Wild-type HeLa and BAX knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32503 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Bax antibody [E63] (ab32503) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: BAX knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human BAX knockout HeLa cell line (Human BAX knockout HeLa cell line ab255363)
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Different batches of ab32503 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.1 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 18 kDa.
All lanes: Western blot - Anti-Bax antibody [E63] (ab32503)
Predicted band size: 21 kDa
Lane 1 = Bax protein (Tagged) (ab85157), 10 ng. Lane 2 = Extract of HeLa cells, 40 ug. Lane 3 = Extract of HepG2 cells, 40 ug. Lane 4 = Bax protein (Tagged) (ab85157), 10 ng. Lane 5 = Extract of HeLa cells, 40 ug. Lane 6 = Extract of HepG2 cells, 40 ug. SDS PAGE performed under reducing conditions (100mM DTT Sample heated at 50oC). Primary : Lanes 1-3: Anti Bax antibody (ab77566) at 1 ug/mL. Lanes 4-6: Anti Bax antibody (ab32503) at 1:2000 dilution. Secondary : Lanes 1-3: Goat anti mouse IgG(H&L)-HRP at 1:10000. Lanes 4-6: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL with 2 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : Bax 21kDa and Bax (Tagged) 49 kDa. Observed band size : Bax 21kDa and Bax (Tagged) 49 kDa.
All lanes: Western blot - Anti-Bax antibody [E63] (ab32503)
Predicted band size: 21 kDa
IHC image of ab32503 staining Bax in rat kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32503, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Blocking and Diluting buffers: 5% NFDM/TBST
Exposure time 1~9 lanes 32 s; 10~11 lanes 3 min
Jurkat is negative reported by PMID: 15528359. Brain is low expressed reported by PMID: 27069530.
All lanes: Western blot - Anti-Bax antibody [E63] (ab32503) at 1/2000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell). Whole cell lysates at 20 µg
Lane 2: Hep G2 (Human hepatocellular carcinoma epithelial cell). Whole cell lysates at 20 µg
Lane 3: Jurkat (Human T cell leukemia T lymphocyte) Whole cell lysates at 20 µg
Lane 4: A549 (Human lung carcinoma epithelial cell) Whole cell lysates at 20 µg
Lane 5: C2C12 (Mouse myoblasts myoblast) Whole cell lysates at 20 µg
Lane 6: C6 (Rat glial tumor glial cell) Whole cell lysates at 20 µg
Lane 7: Mouse brain. Whole tissue lysate at 20 µg
Lanes 8 and 10: Rat brain. Whole tissue lysate at 20 µg
Lanes 9 and 11: Rat Spleen. Whole tissue lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 18 kDa
Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Bax Rabbit Monoclonal Antibody (ab32503) at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Standard Curve for Bax (Analyte: Recombinant human Bax protein (tagged) ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml . Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.
Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling BAX with ab32503 at a concentration of 0.05 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab32503 Anti-Bax antibody [E63] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)
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