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Anti-Bax antibody [E63] (ab32503) is a rabbit monoclonal antibody detecting Bax in Western Blot, IP, IHC-P, ELISA. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 2470 publications

- Trusted since 2006


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503), expandable thumbnail
  • Immunoprecipitation - Anti-Bax antibody [E63] (AB32503), expandable thumbnail
  • Western blot - Anti-Bax antibody [E63] (AB32503), expandable thumbnail
  • Western blot - Anti-Bax antibody [E63] (AB32503), expandable thumbnail
  • Western blot - Anti-Bax antibody [E63] (AB32503), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWBsELISA
Human
Tested
Not recommended
Tested
Not recommended
Tested
Expected
Mouse
Expected
Not recommended
Expected
Not recommended
Tested
Expected
Rat
Tested
Not recommended
Expected
Not recommended
Tested
Expected
Cow
Predicted
Not recommended
Predicted
Not recommended
Predicted
Predicted
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human, Cow, Recombinant full length protein - Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Rat
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Species
Cow
Dilution info
-
Notes

-

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:36361894, PubMed:8358790, PubMed:8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816).

Alternative names

Recommended products

Anti-Bax antibody [E63] (ab32503) is a rabbit monoclonal antibody detecting Bax in Western Blot, IP, IHC-P, ELISA. Suitable for Human, Mouse, Rat.



- KO validated for confirmed specificity

- Biophysical QC for unrivalled batch-batch consistency

- Over 2470 publications

- Trusted since 2006

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
E63
Purification technique
Affinity purification Protein A
Specificity

Expression levels of BAX protein vary with sample type. Induction may be required if endogenous expression is low.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-Bax antibody [E63] (ab32503) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in IHC-P, IP, WB, sELISA in human, mouse, rat samples.

Anti-Bax antibody [E63] (ab32503) has been cited over 2479 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Bax antibody [E63] (ab32503) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

The specificity of Anti-Bax antibody [E63] (ab32503) has been confirmed by testing in knockout samples.

Anti-Bax antibody [E63] (ab32503) has 12 independent reviews from customers.

Anti-Bax antibody [E63] (ab32503) specifically detects Bax (UniProt ID: Q07812; Molecular weight: 21kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone E63 - Anti-Bax antibody [E63] - BSA and Azide free ab216985.

Antibody clone E63 is also available pre-conjugated to a variety of labels for your convenience - HRP (HRP Anti-Bax antibody [E63] ab205822).

The BAX gene (BCL2 Associated X) is a key player in the regulation of apoptosis, or programmed cell death, which is crucial for maintaining cellular homeostasis and preventing cancer. BAX is part of the BCL-2 protein family and promotes apoptosis by increasing mitochondrial membrane permeability, leading to the release of cytochrome c and activation of caspases. In oncology, BAX expression is regulated by the tumor suppressor protein p53, which is often mutated in cancer. Mutations or dysregulation of BAX can contribute to cancer development and progression by allowing cancer cells to survive.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.

Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

Associated diseases and disorders

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

    Purified ab32503 staining Bax in Human lung carcinoma tissue section by immmunohistochemistry (IHC-P- Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraffin and heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0). Samples were incubated with primary antibody at 1:500 dilution. A goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as a secondary antibody at 1:500 dilution. Cytoplasmic staining on human lung carcinoma.

  • Immunoprecipitation - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Immunoprecipitation - Anti-Bax antibody [E63] (ab32503)

    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, 10μg
    Lane 2 (+): HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab32503 in HeLa whole cell lysate

    Purified ab32503 immunoprecipitating Bax in HeLa lysates. For western blotting, the primary antibody used was purified ab32503 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection at 1/1000 dilution. Capture antibody was used at a 1/20 dilution. Blocking and diluting buffer used was 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-Bax antibody [E63] (ab32503)

    Predicted band size: 21 kDa

    Exposure time: 1s

  • Western blot - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Western blot - Anti-Bax antibody [E63] (ab32503)

    Bax Western blot staining using rabbit Anti-Bax antibody

    Lanes 1 - 2: Merged signal (red and green). Green - ab32503. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa or ab18058, observed at 130 kDa.

    This western blot image is a comparison between ab32503 and a competitor's top cited rabbit polyclonal antibody.

    All lanes: Western blot - Anti-Bax antibody [E63] (ab32503)

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: Bax knockout HAP1 cell lysate at 20 µg

    Predicted band size: 21 kDa

  • Western blot - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Western blot - Anti-Bax antibody [E63] (ab32503)

    Bax Western blot staining using rabbit Anti-Bax antibody

    Lanes 1 - 2: Merged signal (red and green). Green - ab32503. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
    ab32503 was shown to recognize Bax in wild-type HAP1 cells, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab32503 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Bax antibody [E63] (ab32503) at 1/1000 dilution

    Lane 1: Wild-type HAP1 cell lysate at 20 µg

    Lane 2: Bax knockout HAP1 cell lysate at 20 µg

    Predicted band size: 21 kDa

  • Western blot - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Western blot - Anti-Bax antibody [E63] (ab32503)

    Bax Western blot staining using rabbit Anti-Bax antibody

    Lanes 1- 2: Merged signal (red and green). Green - ab32503. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab32503 was shown to react with Bax in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BAX knockout HeLa cell line ab255363 (knockout cell lysate Human BAX knockout HeLa cell lysate ab263841) was used. Wild-type HeLa and BAX knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32503 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-Bax antibody [E63] (ab32503) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human BAX knockout HeLa cell lysate (Human BAX knockout HeLa cell lysate ab263841) at 20 µg

    Lane 2: Western blot - Human BAX knockout HeLa cell line (Human BAX knockout HeLa cell line ab255363)

    Performed under reducing conditions.

    Predicted band size: 21 kDa

  • Western blot - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Western blot - Anti-Bax antibody [E63] (ab32503)

    Bax Western blot staining using rabbit Anti-Bax antibody

    Different batches of ab32503 were tested on HeLa (Human cervix adenocarcinoma epithelial cell) lysate at 0.1 µg/ml. 15 µg of lysate was loaded in each lane.

    All lanes: Western blot - Anti-Bax antibody [E63] (ab32503)

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

    IHC image of ab32503 staining Bax in rat kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32503, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Western blot - Anti-Bax antibody [E63] (ab32503)

    Bax Western blot staining using rabbit Anti-Bax antibody

    Blocking and Diluting buffers: 5% NFDM/TBST

    Exposure time 1~9 lanes 32 s; 10~11 lanes 3 min

    Jurkat is negative reported by PMID: 15528359. Brain is low expressed reported by PMID: 27069530.

    All lanes: Western blot - Anti-Bax antibody [E63] (ab32503) at 1/2000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: Hep G2 (Human hepatocellular carcinoma epithelial cell). Whole cell lysates at 20 µg

    Lane 3: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

    Lane 4: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 5: C2C12 (Mouse myoblasts myoblast) whole cell lysates at 20 µg

    Lane 6: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

    Lane 7: Mouse brain whole tissue lysate at 20 µg

    Lanes 8 and 10: Rat brain whole tissue lysate at 20 µg

    Lanes 9 and 11: Rat spleen whole tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

    Immunohistochemical analysis of paraffin-embedded human lymph node using anti-Bax Rabbit Monoclonal Antibody (ab32503) at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Sandwich ELISA - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Sandwich ELISA - Anti-Bax antibody [E63] (ab32503)

    Standard Curve for Bax (Analyte: Recombinant human Bax protein (tagged) ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml . Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (ab32503)

    Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling BAX with ab32503 at a concentration of 0.05 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

    ab32503 Anti-Bax antibody [E63] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

    Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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